LOCUS       sequence-300502-        8628 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast expression plasmid for SARS-CoV-2 S (surface glycoprotein).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8628)
  TITLE     SARS-CoV-2 protein Yeast expression constructs, tagged, for protein 
            expression and/or purification
REFERENCE   2  (bases 1 to 8628)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4046409
FEATURES             Location/Qualifiers
     source          1..8628
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(984..1787)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     primer_bind     2138..2156
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     CDS             complement(2201..2224)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             complement(2225..2242)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     promoter        complement(6073..6514)
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     primer_bind     complement(6079..6102)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    6397..6514
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     terminator      6581..6612
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        6613..6715
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             join(6716..6784,6785..7576)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESLRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLATIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGSLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6934..6953)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             7577..7768
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     terminator      7792..7819
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(7831..8418)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      complement(8440..8469)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     rep_origin      join(8547..8628,1..901)
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
ORIGIN
        1 ggactgtaac gagctactaa aatattgcga ataccgcttc cacaaacatt gctcaaaagt
       61 atctctttgc tatatatctc tgtgctatat ccctatataa cctacccatc cacctttcgc
      121 tccttgaact tgcatctaaa ctcgacctct acatttttta tgtttatctc tagtattact
      181 ctttagacaa aaaaattgta gtaagaacta ttcatagagt gaatcgaaaa caatacgaaa
      241 atgtaaacat ttcctatacg tagtatatag agacaaaata gaagaaaccg ttcataattt
      301 tctgaccaat gaagaatcat caacgctatc actttctgtt cacaaagtat gcgcaatcca
      361 catcggtata gaatataatc ggggatgcct ttatcttgaa aaaatgcacc cgcagcttcg
      421 ctagtaatca gtaaacgcgg gaagtggagt caggcttttt ttatggaaga gaaaatagac
      481 accaaagtag ccttcttcta accttaacgg acctacagtg caaaaagtta tcaagagact
      541 gcattataga gcgcacaaag gagaaaaaaa gtaatctaag atgctttgtt agaaaaatag
      601 cgctctcggg atgcattttt gtagaacaaa aaagaagtat agattctttg ttggtaaaat
      661 agcgctctcg cgttgcattt ctgttctgta aaaatgcagc tcagattctt tgtttgaaaa
      721 attagcgctc tcgcgttgca tttttgtttt acaaaaatga agcacagatt cttcgttggt
      781 aaaatagcgc tttcgcgttg catttctgtt ctgtaaaaat gcagctcaga ttctttgttt
      841 gaaaaattag cgctctcgcg ttgcattttt gttctacaaa atgaagcaca gatgcttcgt
      901 tactcgggta ataactgata taattaaatt gaagctctaa tttgtgagtt tagtatacat
      961 gcatttactt ataatacagt tttttagttt tgctggccgc atcttctcaa atatgcttcc
     1021 cagcctgctt ttctgtaacg ttcaccctct accttagcat cccttccctt tgcaaatagt
     1081 cctcttccaa caataataat gtcagatcct gtagaaacca catcatccac ggttctatac
     1141 tgttgaccca atgcatctcc cttgtcatct aaacccacac cgggtgtcat aatcaaccaa
     1201 tcgtaacctt catctcttcc acccatgtct ctttgagcaa taaagccgat aacaaaatct
     1261 ttgtcgctct tcgcaatgtc aacagtaccc ttagtatatt ctccagtaga tagggagccc
     1321 ttgcatgaca attctgctaa catcaaaagg cctctaggtt cctttgttac ttcttctgcc
     1381 gcctgcttca aaccgctaac aatacctggg cccaccacac cgtgtgcatt cgtaatgtct
     1441 gcccattctg ctattctgta tacacccgca gagtactgca atttgactgt attaccaatg
     1501 tcagcaaatt ttctgtcctc gaagagtaaa aaattgtact tggcggataa tgcctttagc
     1561 ggcttaactg tgccctccat ggaaaaatca gtcaagatat ccacatgtgt ttttagtaaa
     1621 caaattttgg gacctaatgc ttcaactaac tccagtaatt ccttggtggt acgaacatcc
     1681 aatgaagcac acaagtttgt ttgcttttcg tgcatgatat taaatagctt ggcagcaaca
     1741 ggactaggat gagtagcagc acgttcctta tatgtagctt tcgacatgat ttatcttcgt
     1801 ttcctgcacg tttttgttct gtgcagttgg gttaagaata ctgggcaatt tcatgtttct
     1861 tcaacactac atatgcgtat atataccaat ctaagtctgt gctccttcct tcgttcttcc
     1921 ttctgttcgg agattaccga atcaaaaaaa tttcaaggaa accgtgtact gcagtgcact
     1981 agtggttcgg cggccgcttt gtacagaaaa aaaagaaaaa tttgaaatat aaataacgtt
     2041 cttaatacta acataactat taaaaaaaat aaatagggac ctagacttca ggttgtctaa
     2101 ctccttcctt ttcggttaga gcggatgtgg gaggagggcg tgaatgtaag cgtgacataa
     2161 ctaattacat gatatcgaca aaggaaaagg ggcctgttta tttttcaaac tgcggatggc
     2221 tccaagaacc tctcggcacg agtgtgtaat gtaatttgac tcctttgagc actggctcag
     2281 agtcgtcttc atcaaatttg cagcaggatc cacaagaaca acagcccttg agacaactac
     2341 agcaactggt catacagcaa agcataattg tcaccattac tatggcaatc aagccagcta
     2401 taaaacctag ccaaatgtac catggccatt ttatatactg ctcatacttt ccaagttctt
     2461 ggagatcgat gagagattca tttaaattct tggcaacctc attgaggcgg tcaatttctt
     2521 tttgaatgtt tacaactgaa gcattaatgc cagagatgtc acctaaatca acatctggtg
     2581 atgtatgatt cttaaaatat ttatctaact cctccttgaa tgagtctaat tcaggttgca
     2641 aaggatcata aactgtgttg ttgacaattc ctattacaac atcacagtta ccagacacaa
     2701 atgtgttgtc tgtagtaatg atttgtggtt cataaaaatt cctttgtgtt acaaaccagt
     2761 gtgtgccatt tgaaacaaag acaccttcac gaggaaagtg tgcttttcca tcatgacaaa
     2821 tggcaggagc agttgtgaag ttcttttctt gtgcagggac ataagtcaca tgcaagaaga
     2881 ctacaccatg aggtgctgac tgagggaagg acataagatg atagcccttt ccacaaaaat
     2941 caactctttt tgattgtcca agtacacact ctgacatttt agtagcagca agattagcag
     3001 aagctctgat ttctgcagct ctaattaatt gttgagtcac atatgtctgc aaactttgaa
     3061 gtctgcctgt gatcaaccta tcaatttgca cttcagcctc aactttgtca agacgtgaaa
     3121 ggatatcatt taaaacactt gaaattgcac caaaattgga gctaagttgt ttaacaagcg
     3181 tgtttaaagc ttgtgcattt tggttgacca catcttgaag ttttccaagt gcacttgctg
     3241 tggaagaaag tgagtcttga attttgccaa tagcactatt aaattggttg gcaatcaatt
     3301 tttggttctc atagagaaca ttctgtgtaa ctccaatacc attaaaccta taagccattt
     3361 gcatagcaaa tggtatttgt aatgcagcac ctgcaccaaa ggtccaacca gaagtgattg
     3421 tacccgctaa cagtgcagaa gtgtattgag caatcatttc atctgtgagc aaaggtggca
     3481 aaacagtaag gccgttaaac ttttgtgcac aaatgaggtc tctagcagca atatcaccaa
     3541 ggcaatcacc atattgtttg atgaagccag catctgcaag tgtcactttg ttgaaaagta
     3601 gatcttcaat aaatgacctc ttgcttggtt ttgatggatc tggtaatatt tgtgaaaaat
     3661 taaaaccacc aaaatcttta attggtggtg ttttgtaaat ttgtttgact tgtgcaaaaa
     3721 cttcttgggt gtttttgtct tgttcaacag ctattccagt taaagcacgg tttaattgtg
     3781 tacaaaaact gccatattgc aacaaaagat tgctgcattc agttgaatca ccacaaatgt
     3841 acattgtaca atctactgat gtcttggtca tagacactgg tagaatttct gtggtaacac
     3901 taatagtaaa atttgtgggt atggcaatag agttattaga gtaagcaact gaattttctg
     3961 caccaagtga catagtgtag gcaatgatgg attgactagc tacactacgt gcccgccgag
     4021 gagaattagt ctgagtctga taactagcgc atatacctgc accaatgggt atgtcacact
     4081 catatgagtt gttgacatgt tcagccccta ttaaacagcc tgcacgtgtt tgaaaaacat
     4141 tagaacctgt agaataaaca cgccaagtag gagtaagttg atctgcatga atagcaacag
     4201 ggacttctgt gcagttaaca tcctgataaa gaacagcaac ctggttagaa gtatttgttc
     4261 ctggtgttat aacactgaca ccaccaaaag aacatggtgt aatgtcaaga atctcaagtg
     4321 tctgtggatc acggacagca tcagtagtgt cagcaatgtc tctgccaaat tgttggaaag
     4381 gcagaaactt tttgttagac tcagtaagaa cacctgtgcc tgttaaacca ttgaagttga
     4441 aattgacaca tttgttttta accaaattag tagacttttt aggtccacaa acagttgctg
     4501 gtgcatgtag aagttcaaaa gaaagtacta ctactctgta tggttggtaa ccaacaccat
     4561 tagtgggttg gaaaccatat gattgtaaag gaaagtaaca attaaaacct tcaacaccat
     4621 tacaaggtgt gctaccggcc tgatagattt cagttgaaat atctctctca aaaggtttga
     4681 gattagactt cctaaacaat ctatacaggt aattataatt accaccaacc ttagaatcaa
     4741 gattgttaga attccaagct ataacgcagc ctgtaaaatc atctggtaat ttataattat
     4801 aatcagcaat ctttccagtt tgccctggag cgatttgtct gacttcatca cctctaatta
     4861 caaatgaatc tgcatagaca ttagtaaagc agagatcatt taatttagta ggagacactc
     4921 cataacactt aaaagtggaa aatgatgcgg aattatatag gacagaataa tcagcaacac
     4981 agttgctgat tctcttcctg ttccaagcat aaacagatgc aaatctggtg gcgttaaaaa
     5041 cttcaccaaa agggcacaag tttgtaatat taggaaatct aacaatagat tctgttggtt
     5101 ggactctaaa gttagaagtt tgatagattc ctttttctac agtgaaggat ttcaacgtac
     5161 actttgtttc tgagagaggg tcaagtgcac agtctacagc atctgtaatg gttccatttt
     5221 cattatattt taatagaaaa gtcctaggtt gaagataacc cacataataa gctgcagcac
     5281 cagctgtcca acctgaagaa gaatcaccag gagtcaaata acttctatgt aaagcaagta
     5341 aagtttgaaa cctagtgatg ttaataccta ttggcaaatc taccaatggt tctaaagccg
     5401 aaaaaccctg agggagatca cgcactaaat taataggcgt gtgcttagaa tatattttaa
     5461 aataaccatc aatattctta aacacaaatt ccctaagatt tttgaaatta ccctgttttc
     5521 cttcaaggtc cataagaaaa ggctgagaga catattcaaa agtgcaatta ttcgcactag
     5581 aataaactct gaactcactt tccatccaac ttttgttgtt tttgtggtaa taaacaccca
     5641 aaaatggatc attacaaaat tgaaattcac agactttaat aacaacatta gtagcgttat
     5701 taacaataag tagggactgg gtcttcgaat ctaaagtagt accaaaaatc cagcctctta
     5761 ttatgttaga cttctcagtg gaagcaaaat aaacaccatc attaaatggt aggacagggt
     5821 tatcaaacct cttagtacca ttggtcccag agacatgtat agcatggaac caagtaacat
     5881 tggaaaagaa aggtaagaac aagtcctgag ttgaatgtaa aactgaggat ctgaaaactt
     5941 tgtcagggta ataaacacca cgtgtgaaag aattagtgta tgcagggggt aattgagttc
     6001 tggttgtaag attaacacac tgactagaga ctagtggcaa taaaacaaga aaaacaaaca
     6061 ttatagtttt ttctccttga cgttaaagta tagaggtata ttaacaattt tttgttgata
     6121 cttttatgac atttgaataa gaagtaatac aaactgaaaa tgttgaaagt attagttaaa
     6181 gtggttatgc agcttttcca tttatatatc tgttaataga tcaaaaatca tcgcttcgct
     6241 gattaattac cccagaaata aggctaaaaa actaatcgca ttatcatcct atggttgtta
     6301 atttgattcg ttaatttgaa ggtttgtggg gccaggttac tgccaatttt tcctcttcat
     6361 aaccataaaa gctagtattg tagaatcttt attgttcgga gcagtgcggc gcgaggcaca
     6421 tctgcgtttc aggaacgcga ccggtgaaga cgaggacgca cggaggagag tcttccgtcg
     6481 gagggctgtc gcccgctcgg cggcttctaa tccgtacttc aatatagcaa gcggccgctc
     6541 taactgacca tttaaatcat acctgacctc catagcagaa agtcaaaagc ctccgaccgg
     6601 aggcttttga cttgatcggc acgtaagagg ttccaacttt caccataatg aaataagatc
     6661 actaccgggc gtattttttg agttatcgag attttcagga gctaaggaag ctaaaatgag
     6721 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     6781 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     6841 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagtttac gccccgaaga
     6901 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     6961 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     7021 gtactcacca gtcacagaaa agcatctcac ggatggcatg acagtaagag aattatgcag
     7081 tgctgccata accatgagtg ataacactgc ggccaactta cttctggcaa cgatcggagg
     7141 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     7201 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     7261 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     7321 gcaacaatta atagactgga tggaggcgga taaagttgca ggatcacttc tgcgctcggc
     7381 cctcccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     7441 tatcattgca gcactggggc cagatggtaa gccctcccgc atcgtagtta tctacacgac
     7501 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     7561 gattaagcat tggtaagtga ccaaacagga aaaaaccgcc cttaacatgg cccgctttat
     7621 cagaagccag acattaacgc ttctggagaa actcaacgag ctggacgcgg atgaacaggc
     7681 agacatctgt gaatcgcttc acgaccacgc tgatgagctt taccgcagct gcctcgcgcg
     7741 tttcggtgat gacggtgaaa acctctgatg agggcccaaa tgtaatcacc tggctcacct
     7801 tcgggtgggc ctttctgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     7861 tcacaaaaat cgatgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     7921 ggcgtttacc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     7981 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     8041 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     8101 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     8161 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     8221 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     8281 tggtatctgc gctctgctga agccagttac ctcggaaaaa gagttggtag ctcttgatcc
     8341 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     8401 agaaaaaaag gatctcaaga agatcctttg attttctacc gaagaaaggc ccacccgtga
     8461 aggtgagcca gtgagttgat tgcagtccag ttacgctgga gtcaagcttg taacacgcgg
     8521 ccgctcggga agttcctatt ctctagctag aaagtatagg aacttcagag cgcttttgaa
     8581 aaccaaaagc gctctgatga cgcactttca aaaaaccaaa aacgcacc
//
