LOCUS       sequence-300510-       10991 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Modified Tobacco rattle virus RNA2 vector that could be used for 
            expression of sgRNAs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10991)
  AUTHORS   Ellison EE, Nagalakshmi U, Gamo ME, Huang PJ, Dinesh-Kumar S, Voytas
            DF
  TITLE     Multiplexed heritable gene editing using RNA viruses and mobile 
            single guide RNAs.
  JOURNAL   Nat Plants. 2020 Jun 1. pii: 10.1038/s41477-020-0670-y. doi: 
            10.1038/s41477-020-0670-y.
  PUBMED    32483329
REFERENCE   2  (bases 1 to 10991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Plants.
            2020 Jun 1. pii: 10.1038/s41477-020-0670-y. doi: 
            10.1038/s41477-020-0670-y."
COMMENT     Sequence Label: SPDK3888(TRV-RNA2-pPEBV-MCS-AtIleu-tRNA)
FEATURES             Location/Qualifiers
     source          1..10991
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        427..772
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     715..735
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             2921..2938
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      2973..3225
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    3247..3271
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4572..5201
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5630..6703
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      6769..6963
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(7199..7221)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7307..7447
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7362..7381
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7462..7479)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7633..8221)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7713..7732)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9646..10440)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    10865..10889
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 aagcttgcat gcctgcaggt caacatggtg gagcacgaca ctctcgtcta ctccaagaat
       61 atcaaagata cagtctcaga agaccagagg gctattgaga cttttcaaca aagggtaata
      121 tcgggaaacc tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta
      181 gaaaaggaag atggcttcta caaatgccat cattgcgata aaggaaaggc tatcgttcaa
      241 gatgcctcta ccgacagtgg tcccaaagat ggacccccac ccacgaggaa catcgtggaa
      301 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgatgg tcaacatggt
      361 ggagcacgac actctcgtct actccaagaa tatcaaagat acagtctcag aagaccagag
      421 ggctattgag acttttcaac aaagggtaat atcgggaaac ctcctcggat tccattgccc
      481 agctatctgt cacttcatcg aaaggacagt agaaaaggaa gatggcttct acaaatgcca
      541 tcattgcgat aaaggaaagg ctatcgttca agatgcctct accgacagtg gtcccaaaga
      601 tggaccccca cccacgagga acatcgtgga aaaagaagac gttccaacca cgtcttcaaa
      661 gcaagtggat tgatgtgata tctccactga cgtaagggat gacgcacaat cccactatcc
      721 ttcgcaagac ccttcctcta tataaggaag ttcatttcat ttggagagga taaaacattg
      781 cacctatggt gttgccctgg ctggggtatg tcagtgatcg cagtagaatg tactaattga
      841 caagttggag aatacggtag aacgtcctta tccaacacag cctttatccc tctccctgac
      901 gaggtttttg tcagtgtaat atttcttttt gaactatcca gcttagtacc gtacgggaaa
      961 gtgactggtg tgcttatctt tgaaatgtta ctttgggttt cggttcttta ggttagtaag
     1021 aaagcacttg tcttctcata caaaggaaaa cctgagacgt atcgcttacg aaagtagcaa
     1081 tgaaagaaag gtggtggttt taatcgctac cgcaaaaacg atggggtcgt tttaattaac
     1141 ttctcctacg caagcgtcta aacggacgtt ggggttttgc tagtttcttt agagaaaact
     1201 agctaagtct ttaatgttat cattagagat ggcataaata taatacttgt gtctgctgat
     1261 aagatcattt taatttggac gattagactt gttgaactac aggttactga atcacttgcg
     1321 ctaatcaaca tgggagatat gtacgatgaa tcatttgaca agtcgggcgg tcctgctgac
     1381 ttgatggacg attcttgggt ggaatcagtt tcgtggaaag atctgttgaa gaagttacac
     1441 agcataaaat ttgcactaca gtctggtaga gatgagatca ctgggttact agcggcactg
     1501 aatagacagt gtccttattc accatatgag cagtttccag ataagaaggt gtatttcctt
     1561 ttagactcac gggctaacag tgctcttggt gtgattcaga acgcttcagc gttcaagaga
     1621 cgagctgatg agaagaatgc agtggcgggt gttacaaata ttcctgcgaa tccaaacaca
     1681 acggttacga cgaaccaagg gagtactact actaccaagg cgaacactgg ctcgactttg
     1741 gaagaagact tgtacactta ttacaaattc gatgatgcct ctacagcttt ccacaaatct
     1801 ctaacttcgt tagagaacat ggagttgaag agttattacc gaaggaactt tgagaaagta
     1861 ttcgggatta agtttggtgg agcagctgct agttcatctg caccgcctcc agcgagtgga
     1921 ggtccgatac gtcctaatcc ctagggattt aaggacgtga actctgttga gatctctgtg
     1981 aaattcagag ggtgggtgat accatattca ctgatgccat tagcgacatc taaatagggc
     2041 taattgtgac taatttgagg gaatttcctt taccattgac gtcagtgtcg ttggtagcat
     2101 ttgagtttcg gcacacaagg ttaaaaacgc tgtagtaata catgcgcaag aacaggctga
     2161 gcatcttgtt ctggggtttc acactatctt tagagaaagt gttaagttaa ttaagttatc
     2221 ttaattaaga gcataattat actgatttgt ctctcgttga tagagtctat cattctgtta
     2281 ttaaaaattt gacaactcgg tttgctgacc tactggtcac tgtatcactt acccgagtta
     2341 acgagtctag aaggcctcca tggggatccg gtaccgagct ctgagctccc gtagctcagt
     2401 tggttagagc gttggtctta tgagccgaag gtcgcgggtt cgagccccgc cggaagcacc
     2461 cgggcatgtc ccgaagacat taaactacgg ttctttaagt agatccgtgt ctgaagtttt
     2521 aggttcaatt taaacctacg agattgacat tctcgactga tcttgattga tcggtaagtc
     2581 ttttgtaatt taattttctt tttgatttta ttttaaattg ttatctgttt ctgtgtatag
     2641 actgtttgag atcggcgttt ggccgactca ttgtcttacc ataggggaac ggactttgtt
     2701 tgtgttgtta ttttatttgt attttattaa aattctcaac gatctgaaaa agcctcgcgg
     2761 ctaagagatt gttggggggt gagtaagtac ttttaaagtg atgatggtta caaaggcaaa
     2821 aggggtaaaa cccctcgcct acgtaagcgt tattacgccc gtctgtactt atatcagtac
     2881 actgacgagt ccctaaagga cgaaacggga gaacgctagc caccaccacc accaccacgt
     2941 gtgaattaca ggtgaccagc tcgaatttcc ccgatcgttc aaacatttgg caataaagtt
     3001 tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt ctgttgaatt
     3061 acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga tgggttttta
     3121 tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata tagcgcgcaa
     3181 actaggataa attatcgcgc gcggtgtcat ctatgttact agatcgggaa ttaaactatc
     3241 agtgtttgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt attagaataa
     3301 tcggatattt aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat gtgcatgcca
     3361 accacagggt tcccctcggg atcaaagtac tttgatccaa cccctccgct gctatagtgc
     3421 agtcggcttc tgacgttcag tgcagccgtc ttctgaaaac gacatgtcgc acaagtccta
     3481 agttacgcga caggctgccg ccctgccctt ttcctggcgt tttcttgtcg cgtgttttag
     3541 tcgcataaag tagaatactt gcgactagaa ccggagacat tacgccatga acaagagcgc
     3601 cgccgctggc ctgctgggct atgcccgcgt cagcaccgac gaccaggact tgaccaacca
     3661 acgggccgaa ctgcacgcgg ccggctgcac caagctgttt tccgagaaga tcaccggcac
     3721 caggcgcgac cgcccggagc tggccaggat gcttgaccac ctacgccctg gcgacgttgt
     3781 gacagtgacc aggctagacc gcctggcccg cagcacccgc gacctactgg acattgccga
     3841 gcgcatccag gaggccggcg cgggcctgcg tagcctggca gagccgtggg ccgacaccac
     3901 cacgccggcc ggccgcatgg tgttgaccgt gttcgccggc attgccgagt tcgagcgttc
     3961 cctaatcatc gaccgcaccc ggagcgggcg cgaggccgcc aaggcccgag gcgtgaagtt
     4021 tggcccccgc cctaccctca ccccggcaca gatcgcgcac gcccgcgagc tgatcgacca
     4081 ggaaggccgc accgtgaaag aggcggctgc actgcttggc gtgcatcgct cgaccctgta
     4141 ccgcgcactt gagcgcagcg aggaagtgac gcccaccgag gccaggcggc gcggtgcctt
     4201 ccgtgaggac gcattgaccg aggccgacgc cctggcggcc gccgagaatg aacgccaaga
     4261 ggaacaagca tgaaaccgca ccaggacggc caggacgaac cgtttttcat taccgaagag
     4321 atcgaggcgg agatgatcgc ggccgggtac gtgttcgagc cgcccgcgca cgtctcaacc
     4381 gtgcggctgc atgaaatcct ggccggtttg tctgatgcca agctggcggc ctggccggcc
     4441 agcttggccg ctgaagaaac cgagcgccgc cgtctaaaaa ggtgatgtgt atttgagtaa
     4501 aacagcttgc gtcatgcggt cgctgcgtat atgatgcgat gagtaaataa acaaatacgc
     4561 aaggggaacg catgaaggtt atcgctgtac ttaaccagaa aggcgggtca ggcaagacga
     4621 ccatcgcaac ccatctagcc cgcgccctgc aactcgccgg ggccgatgtt ctgttagtcg
     4681 attccgatcc ccagggcagt gcccgcgatt gggcggccgt gcgggaagat caaccgctaa
     4741 ccgttgtcgg catcgaccgc ccgacgattg accgcgacgt gaaggccatc ggccggcgcg
     4801 acttcgtagt gatcgacgga gcgccccagg cggcggactt ggctgtgtcc gcgatcaagg
     4861 cagccgactt cgtgctgatt ccggtgcagc caagccctta cgacatatgg gccaccgccg
     4921 acctggtgga gctggttaag cagcgcattg aggtcacgga tggaaggcta caagcggcct
     4981 ttgtcgtgtc gcgggcgatc aaaggcacgc gcatcggcgg tgaggttgcc gaggcgctgg
     5041 ccgggtacga gctgcccatt cttgagtccc gtatcacgca gcgcgtgagc tacccaggca
     5101 ctgccgccgc cggcacaacc gttcttgaat cagaacccga gggcgacgct gcccgcgagg
     5161 tccaggcgct ggccgctgaa attaaatcaa aactcatttg agttaatgag gtaaagagaa
     5221 aatgagcaaa agcacaaaca cgctaagtgc cggccgtccg agcgcacgca gcagcaaggc
     5281 tgcaacgttg gccagcctgg cagacacgcc agccatgaag cgggtcaact ttcagttgcc
     5341 ggcggaggat cacaccaagc tgaagatgta cgcggtacgc caaggcaaga ccattaccga
     5401 gctgctatct gaatacatcg cgcagctacc agagtaaatg agcaaatgaa taaatgagta
     5461 gatgaatttt agcggctaaa ggaggcggca tggaaaatca agaacaacca ggcaccgacg
     5521 ccgtggaatg ccccatgtgt ggaggaacgg gcggttggcc aggcgtaagc ggctgggttg
     5581 cctgccggcc ctgcaatggc actggaaccc ccaagcccga ggaatcggcg tgagcggtcg
     5641 caaaccatcc ggcccggtac aaatcggcgc ggcgctgggt gatgacctgg tggagaagtt
     5701 gaaggccgcg caggccgccc agcggcaacg catcgaggca gaagcacgcc ccggtgaatc
     5761 gtggcaagcg gccgctgatc gaatccgcaa agaatcccgg caaccgccgg cagccggtgc
     5821 gccgtcgatt aggaagccgc ccaagggcga cgagcaacca gattttttcg ttccgatgct
     5881 ctatgacgtg ggcacccgcg atagtcgcag catcatggac gtggccgttt tccgtctgtc
     5941 gaagcgtgac cgacgagctg gcgaggtgat ccgctacgag cttccagacg ggcacgtaga
     6001 ggtttccgca gggccggccg gcatggccag tgtgtgggat tacgacctgg tactgatggc
     6061 ggtttcccat ctaaccgaat ccatgaaccg ataccgggaa gggaagggag acaagcccgg
     6121 ccgcgtgttc cgtccacacg ttgcggacgt actcaagttc tgccggcgag ccgatggcgg
     6181 aaagcagaaa gacgacctgg tagaaacctg cattcggtta aacaccacgc acgttgccat
     6241 gcagcgtacg aagaaggcca agaacggccg cctggtgacg gtatccgagg gtgaagcctt
     6301 gattagccgc tacaagatcg taaagagcga aaccgggcgg ccggagtaca tcgagatcga
     6361 gctagctgat tggatgtacc gcgagatcac agaaggcaag aacccggacg tgctgacggt
     6421 tcaccccgat tactttttga tcgatcccgg catcggccgt tttctctacc gcctggcacg
     6481 ccgcgccgca ggcaaggcag aagccagatg gttgttcaag acgatctacg aacgcagtgg
     6541 cagcgccgga gagttcaaga agttctgttt caccgtgcgc aagctgatcg ggtcaaatga
     6601 cctgccggag tacgatttga aggaggaggc ggggcaggct ggcccgatcc tagtcatgcg
     6661 ctaccgcaac ctgatcgagg gcgaagcatc cgccggttcc taatgtacgg agcagatgct
     6721 agggcaaatt gccctagcag gggaaaaagg tcgaaaaggt ctctttcctg tggatagcac
     6781 gtacattggg aacccaaagc cgtacattgg gaaccggaac ccgtacattg ggaacccaaa
     6841 gccgtacatt gggaaccggt cacacatgta agtgactgat ataaaagaga aaaaaggcga
     6901 tttttccgcc taaaactctt taaaacttat taaaactctt aaaacccgcc tggcctgtgc
     6961 ataactgtct ggccagcgca cagccgaaga gctgcaaaaa gcgcctaccc ttcggtcgct
     7021 gcgctcccta cgccccgccg cttcgcgtcg gcctatcgcg gccgctggcc gctcaaaaat
     7081 ggctggccta cggccaggca atctaccagg gcgcggacaa gccgcgccgt cgccactcga
     7141 ccgccggcgc ccacatcaag gcaccctgcc tcgcgcgttt cggtgatgac ggtgaaaacc
     7201 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
     7261 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc
     7321 agtcacgtag cgatagcgga gtgtatactg gcttaactat gcggcatcag agcagattgt
     7381 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
     7441 catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     7501 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     7561 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     7621 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     7681 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     7741 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     7801 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     7861 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     7921 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     7981 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     8041 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     8101 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     8161 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     8221 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     8281 agggctgatg aatcccctaa tgatttttat caaaatcatt aagttaaggt agatacacat
     8341 cttgtcatat gatcaaatgg tttcgccaaa aatcaataat cagacaacaa aatgtgcgaa
     8401 ctcgatattt tacacgactc tctttaccaa ttctgccccg aattacactt aaaacgactc
     8461 aacagcttaa cgttggcttg ccacgcctta cttgactgta aaactctcac tcttaccgaa
     8521 cttggccgta acctgccaac caaagcgaga acaaaacata acatcaaacg aatcgaccga
     8581 ttgttaggta atcgtcacct ccacaaagag cgactcgctg tataccgttg gcatgctagc
     8641 tttatctgtt cgggcaatac gatgcccatt gtacttgttg actggtctga tatccgtgag
     8701 caaaaacggc ttatggtatt gcgagcttca gtcgcactac acggtcgttc tgttactctt
     8761 tatgagaaag cgttcccgct ttcagagcaa tgttcaaaga aagctcatga ccaatttcta
     8821 gccgaccttg cgagcattct accgagtaac accacaccgc tcattgtcag tgatgctggc
     8881 tttaaagtgc catggtataa atccgttgag aagctgggtt ggtactggtt aagtcgagta
     8941 agaggaaaag tacaatatgc agacctagga gcggaaaact ggaaacctat cagcaactta
     9001 catgatatgt catctagtca ctcaaagact ttaggctata agaggctgac taaaagcaat
     9061 ccaatctcat gccaaattct attgtataaa tctcgctcta aaggccgaaa aaatcagcgc
     9121 tcgacacgga ctcattgtca ccacccgtca cctaaaatct actcagcgtc ggcaaaggag
     9181 ccatggattc tagcaactaa cttacctgtt gaaattcgaa cacccaaaca acttgttaat
     9241 atctattcga agcgaatgca gattgaagaa accttccgag acttgaaaag tcctgcctac
     9301 ggactaggcc tacgccatag ccgaacgagc agctcagagc gttttgatat catgctgcta
     9361 atcgccctga tgcttcaact aacatgttgg cttgcgggcg ttcatgctca gaaacaaggt
     9421 tgggacaagc acttccaggc taacacagtc agaaatcgaa acgtactctc aacagttcgc
     9481 ttaggcatgg aagttttgcg gcattctggc tacacaataa caagggaaga ctcactcgtg
     9541 gctgcaaccc tgcttactca aaatctattc acacatggtt acgttttggg gaaattatga
     9601 ggggatctct cagcgttaag ggattttggt catgcattct aggtactaaa acaattcatc
     9661 cagtaaaata taatatttta ttttctccca atcaggcttg atccccagta agtcaaaaaa
     9721 tagctcgaca tactgttctt ccccgatatc ctccctgatc gaccggacgc agaaggcaat
     9781 gtcataccac ttgtccgccc tgccgcttct cccaagatca ataaagccac ttactttgcc
     9841 atctttcaca aagatgttgc tgtctcccag gtcgccgtgg gaaaagacaa gttcctcttc
     9901 gggcttttcc gtctttaaaa aatcatacag ctcgcgcgga tctttaaatg gagtgtcttc
     9961 ttcccagttt tcgcaatcca catcggccag atcgttattc agtaagtaat ccaattcggc
    10021 taagcggctg tctaagctat tcgtataggg acaatccgat atgtcgatgg agtgaaagag
    10081 cctgatgcac tccgcataca gctcgataat cttttcaggg ctttgttcat cttcatactc
    10141 ttccgagcaa aggacgccat cggcctcact catgagcaga ttgctccagc catcatgccg
    10201 ttcaaagtgc aggacctttg gaacaggcag ctttccttcc agccatagca tcatgtcctt
    10261 ttcccgttcc acatcatagg tggtcccttt ataccggctg tccgtcattt ttaaatatag
    10321 gttttcattt tctcccacca gcttatatac cttagcagga gacattcctt ccgtatcttt
    10381 tacgcagcgg tatttttcga tcagtttttt caattccggt gatattctca ttttagccat
    10441 ttattatttc cttcctcttt tctacagtat ttaaagatac cccaagaagc taattataac
    10501 aagacgaact ccaattcact gttccttgca ttctaaaacc ttaaatacca gaaaacagct
    10561 ttttcaaagt tgttttcaaa gttggcgtat aacatagtat cgacggagcc gattttgaaa
    10621 ccgcggtgat cacaggcagc aacgctctgt catcgttaca atcaacatgc taccctccgc
    10681 gagatcatcc gtgtttcaaa cccggcagct tagttgccgt tcttccgaat agcatcggta
    10741 acatgagcaa agtctgccgc cttacaacgg ctctcccgct gacgccgtcc cggactgatg
    10801 ggctgcctgt atcgagtggt gattttgtgc cgagctgccg gtcggggagc tgttggctgg
    10861 ctggtggcag gatatattgt ggtgtaaaca aattgacgct tagacaactt aataacacat
    10921 tgcggacgtt tttaatgtac tgaattaacg ccgaattaat tcctaggcca ccatgttggg
    10981 cccggcgcgc c
//
