LOCUS       sequence-300512-       12515 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Same as pLz37 but with CsoS2 truncated to remove the entire NTD 
            (i.e. N1, N2, N3, and N4 are removed).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12515)
  AUTHORS   Oltrogge LM, Chaijarasphong T, Chen AW, Bolin ER, Marqusee S, Savage
            DF
  TITLE     Multivalent interactions between CsoS2 and Rubisco mediate 
            alpha-carboxysome formation.
  JOURNAL   Nat Struct Mol Biol. 2020 Mar;27(3):281-287. doi: 
            10.1038/s41594-020-0387-7. Epub 2020 Mar 2.
  PUBMED    32123388
REFERENCE   2  (bases 1 to 12515)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41594-020-0387-7"; journalName: "Nat Struct Mol Biol"; 
            date: "2020-03"; volume: "27"; issue: "3"; pages: "281-287"
COMMENT     Sequence Label: pLz78_CB_operon_CsoS2_delNTD
FEATURES             Location/Qualifiers
     source          1..12515
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      114..200
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      292..319
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     protein_bind    341..360
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     promoter        join(375..380,381..398,399..405)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    413..429
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             8221..8880
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(8287..8306)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        8945..9022
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             9023..10105
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(9042..9061)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     rep_origin      complement(11087..11675)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11167..11186)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggctgagacg atgcgaacct tctccagctt cagccgtggc tcccagcgat cgagcgcttc
       61 agctgtggcc gccaccaggt caacgatgag ggactggttg atcggctagt catcaaataa
      121 aacgaaaggc tcagtcggaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
      181 ctctcctgag taggacaaat ccgccgggag cggatttgaa cgttgcgaag caacggcccg
      241 gagggtggcg ggcaggacgc ccgccataaa ctgccaggca tcaaattaag cagaaggcca
      301 tcctgacgga tggccttttt gcgtttctac tctagtttcg aattgtgagc gctcacaatt
      361 tcgaaacccc aggcttgaca ctttatgctt ccggctcgta taatgtgtgg aattgtgagc
      421 ggataacaat ttcagctagg tgcgcgaatc ccccatcctt tcaggaggaa ctcatggcag
      481 ttaaaaagta tagtgctggt gtaaaagaat accggcagac ctattggatg ccggaataca
      541 caccgttgga ttccgacatc cttgcatgct tcaaaatcac cccacaaccg ggtgttgatc
      601 gcgaagaagc cgcagccgcg gttgcagcag aatcttcaac cggcacatgg accaccgtgt
      661 ggaccgattt gctgaccgac atggactact acaaaggccg tgcctatcgc attgaagacg
      721 tacccggtga cgatgcggca ttctatgcct ttatcgccta cccaatcgac ctgttcgaag
      781 aagggtcagt tgttaacgtg tttacctcac tggttggtaa cgtgttcggc ttcaaagcgg
      841 tacgcggcct gcgtctggaa gatgttcgct tcccactcgc ctacgttaaa acctgtggcg
      901 gcccaccgca cggtattcaa gtcgaacgcg acaagatgaa caaatatggt cgcccactgt
      961 tgggttgcac catcaagcca aaacttggtt tgtctgcgaa aaactacggc cgtgccgtat
     1021 acgagtgcct ccgtggcggc ctcgacttca ctaaagatga tgaaaacatc aattctcagc
     1081 cgttcatgcg ctggcgcgat cgcttcttgt tcgtacaaga cgcgaccgaa actgctgaag
     1141 cccaaaccgg cgaacgcaaa ggccattacc tcaacgtaac ggcgccaact cctgaagaaa
     1201 tgtacaagcg cgcagaattc gccaaagaaa ttggcgcgcc aatcattatg cacgactaca
     1261 tcaccggtgg cttcacggcc aacactggct tggccaagtg gtgtcaagac aacggcgtac
     1321 tgctgcacat ccaccgtgcg atgcatgcgg ttatcgaccg taacccgaac cacggtattc
     1381 acttccgtgt tctgaccaag attctgcgtt tgtcgggtgg cgatcacctg cacaccggta
     1441 ccgttgtcgg caaactggaa ggcgaccgtg cctctactct gggctggatt gatttgctcc
     1501 gcgaatcgtt tatccctgaa gatcgctcgc gcggtatctt cttcgatcaa gactggggtt
     1561 caatgccagg cgtattcgct gtggcctctg gtggtattca cgtatggcac atgcctgcgc
     1621 tggttaacat ctttggtgac gactctgtcc tccaattcgg tggcggtacg ctgggtcatc
     1681 catggggcaa cgctgccggt gctgctgcca accgtgttgc tctggaagcc tgcgtagaag
     1741 cgcgtaacca aggccgcgat atcgaaaaag aaggcaaaga aattctgact gctgctgcac
     1801 agcacagccc agaactgaag attgccatgg aaacttggaa agagatcaaa ttcgaatttg
     1861 acactgtcga caaactcgac actcaaaatc gttgatccct cgtaccacac aacatactaa
     1921 ggtgagtaac catggctgaa atgcaggatt acaagcaaag cctcaaatat gagactttct
     1981 cttatcttcc acccatgaac gcggaacgca tccgcgctca aatcaagtac gcaattgctc
     2041 aaggctggag ccccggcatt gagcacgtag aagtgaaaaa ctccatgaac caatattggt
     2101 acatgtggaa acttcccttc ttcggcgaac aaaatgtcga caacgtgttg gctgaaattg
     2161 aagcgtgtcg tagtgcgtat ccaacacacc aggtcaaact ggtggcttat gacaactatg
     2221 cgcaaagctt aggtctggcc ttcgtggtct accgcggcaa ctaagtcaag tctgtcattg
     2281 cgctgtgcgt ttctctacgc acagcactta ttcaagatgg ggtcaaacat gggcacagca
     2341 ccttcctgca agccaacagg ccgtgtcaaa aattctgtac agtcgggcaa cgcagcgcca
     2401 aaaaaggttg aaaccggtca caccctatcc ggcggatctg ttacgggcac gcaagtggat
     2461 cgtaaatctc atgtgactgg aaacgagccg ggcacttgcc gagcagtcac gggcaccgag
     2521 tacgtaggta ctgagcaatt cacctctttt tgcaatacca gccccaagcc aaatgcgacg
     2581 aaggtcaatg tgaccacaac ggctcgtggt cgccctgtta gcggtacgga agtttcacgg
     2641 accgagaagg taactggcaa cgaatccggt gtctgccgta acgttaccgg caccgaatac
     2701 atgagtaatg aagctcactt ttctctatgt ggaacagccg caaagccttc acaagcggat
     2761 aaagtcatgt tcggcgccac agcacgaacg catcaagtgg tcagtggcag tgatgaattc
     2821 aggccctctt ctgttacggg taacgaatcg ggtgcaaaac gcacaattac cggctcgcag
     2881 tacgcagacg aaggtcttgc gcgactcacg atcaacggag cacctgcaaa agtagccaga
     2941 acccacacct ttgcgggctc tgacgttacc ggcacggaaa tcggtcgctc tactcgcgta
     3001 actggtgatg aaagcggttc gtgtcgttca atctcaggca ccgagtatct cagtaacgag
     3061 caattccaat ccttttgtga cacaaaacct caacgcagcc cgttcaaggt tggccaagat
     3121 cgcacgaaca agggtcagtc tgtgactggt aacttggttg atcgttccga actggttaca
     3181 ggtaacgaac caggttcatg ctctcgggtt acaggctctc agtatggcca aagcaaaatc
     3241 tgcggtggtg gcgtgggaaa agtgcgctca atgcgcaccc ttcgcggcac ctcagtatct
     3301 ggccaacagc tagatcatgc cccaaagatg tccggtgacg agcgcggcgg gtgcatgccc
     3361 gtcaccggta atgagtacta cggtcgtgaa catttcgaac cgttttgtac gagcacccca
     3421 gagcccgaag ctcaatcaac tgaacaatca ttgacctgtg aaggacaaat tattagcggc
     3481 acttcagttg acgccagtga tttggtcaca ggaaatgaaa tcggtgaaca gcaactcatc
     3541 agcggtgacg cctatgttgg cgcgcagcag acaggttgcc ttcccactag tccacgcttc
     3601 aaccaaactg gcaatgttca gtcaatgggt tttaagaaca ccaatcagcc agaacaaaac
     3661 tttgcaccag gtgaagtaat gcctactgac tttagtattc aaaccccagc tcgctcggct
     3721 cagaatcgca ttacaggtaa cgacattgcg ccctcaggtc gcattacagg ccctggtatg
     3781 ctggcaaccg gcttgattac aggaaccccc gaattcaggc acgctgcgcg cgagttggtt
     3841 ggttctccac aacccatggc aatggccatg gccaaccgta ataaggctgc tcaagcacct
     3901 gttgtgcagc cagaagtggt tgcaactcag gaaaagcctg agttggtatg tgcaccaaga
     3961 agcgatcaaa tggatcgtgt gagtggcgaa ggcaaagaac gttgccacat cactggcgat
     4021 gactggtcag ttaacaagca catcaccggt acagccggtc aatgggcgag tggtcgcaac
     4081 ccttccatgc gcggtaatgc gcgtgtggtc gaaaccagcg cgtttgccaa tcgcaatgtg
     4141 ccaaaacctg aaaagccggg ctccaagatc acgggcagta gtggtaatga cacccaaggt
     4201 agtctgatca cttactccgg cggcgcgcgc ggttgagcgt taagtaaagt gtaacgatca
     4261 tgaacacccg taacacacga agcaagcaac gcgcaccgtt tggtgttagc tcatcagtca
     4321 aacctcggct tgacttgatt gagcaagcac caaaccctgc ctatgaccgc catcctgctt
     4381 gcataacgtt gcctgagcgt acctgccggc acccgttaac tgaccttgaa gccaacgaac
     4441 aactgggtcg ttgcgaggat agcgtcaaga accgttttga tcgcgttatc cctttcttgc
     4501 aagttgttgc tggcattcct cttggtctgg attatgttac ccgtgttcaa gaattagccc
     4561 agtcgtcgct tggacatacg ctgcccgaag aattactcaa agataattgg atcagtgggc
     4621 acaatttaaa aggcattttt ggctacgcga ctgctaaagc actaacagca gccacggaac
     4681 aattcagtcg taaaataatg tctgagaagg acgattccgc atcggccatt ggcttcttcc
     4741 tggattgcgg gttccacgca gtggacataa gcccttgtgc cgatggtcgt ctcaaagggt
     4801 tactgcctta catattgcgt ttgcccctta cggcattcac ctatcgtaaa gcctacgcag
     4861 gctcgatgtt cgatattgaa gatgatctgg cacagtggga gaaaaatgaa ctccgccgtt
     4921 atcgtgaagg tgttccaaat acagcggatc agccaacacg atacctgaaa attgctgtgt
     4981 atcatttcag cacctctgac ccgacacact ctggctgcgc ggcacacggc agtaatgatc
     5041 gtgcagcact ggaagcggct ttaacccagc tgatgaaatt cagagaagcg gttgaaaatg
     5101 cccattgctg cggcgcaagt atcgatattt tactgattgg cgttgatacg gatacggatg
     5161 ccattcgcgt tcatattccg gatagcaaag gttttttgaa tccgtatcgt tatgttgaca
     5221 acacagtaac ttatgcgcaa acactacatc tggcgccgga tgaggctcgt gtgattattc
     5281 acgaagcaat tctcaacgca aaccgcagcg atggttgggc taaaggaaat ggagtagcca
     5341 gcgaggggat gcgtcgtttt attggtcagc ttttgatcaa caacctctcg caaatcgatt
     5401 acgtagtaaa tcgtcatggt ggtcgctatc cacccaatga tattggtcat gctgagcgat
     5461 atatcagtgt tggtgatggt tttgatgaag ttcaaatccg gaatttagcc tactacgcgc
     5521 atttggatac ggttgaagaa aatgcgattg atgtggatgt gggaatcaaa attttcacca
     5581 aacttaattt gagtcgaggt ttaccgattc cgattgccat ccactatcgc tatgacccca
     5641 atgttccagg ctccagagaa agaaccgtgg tcaaagcaag acggatatac aacgccatta
     5701 aagagcggtt ctcatccttg gatgagcaga atctattgca gtttcgtttg agcgttcagg
     5761 cgcaggatat cggaagcccg attgaagagg ttgcatccgc atgaaaatca tgcaagttga
     5821 gaaaacgttg gtttcaacaa accgtattgc tgatatgggt cacaaaccac tattagtggt
     5881 atgggagaaa ccgggcgcgc ccaggcaggt cgccgtggat gcgattggct gcataccggg
     5941 cgattgggtt ttgtgcgttg ggtcatcggc agcacgagag gctgcaggaa gcaagtctta
     6001 tccctctgat ttgacgatta tcgggattat tgatcagtgg aatggtgagt aatggaagta
     6061 atgcgcgttc gttccgacct aatcgcaaca cgcaggattc ccggtcttaa aaatatctct
     6121 ttgcgtgtca tggaggatgc tacgggtaag gtcagtgtcg cttgcgatcc cattggcgtt
     6181 cccgagggat gttgggtctt tacgattagc ggctctgccg ctcggtttgg cgtgggtgat
     6241 tttgagattc tcacggattt gacgattggt ggcatcatcg atcactgggt aacttgacca
     6301 tcgctagatg agttgatttt gaatgagtct ttattgagga gagaagaaat ggcagcagta
     6361 acaggtattg cactgggtat gattgaaaca cgtggtctgg ttccagcgat tgaagctgcc
     6421 gatgccatga ccaaggccgc cgaagtacgt ttggttggcc gtcaatttgt tggtggtggt
     6481 tacgtgaccg ttttggtccg tggtgaaacc ggtgccgtca acgcagcagt tcgtgcgggc
     6541 gctgatgcct gcgaacgagt cggcgatggt ctggtcgcgg cgcatatcat tgcccgtgtc
     6601 cattccgaag tcgaaaacat cctgccgaaa gcccctgaag cttaaggatt gggaaagacg
     6661 aaccggcgca ggcttgttcc ggttctttgc ataaagtgac agcttaggag tttatttaaa
     6721 tggctgatgt aactggtatt gctctgggta tgatcgaaac acgtggctta gttcctgcga
     6781 ttgaagcagc ggacgccatg actaaagcgg ctgaagtgcg tttggtcggt cgtcaatttg
     6841 ttggtggcgg ttacgtcacc gtattggttc ggggcgaaac aggcgctgtt aacgccgctg
     6901 ttcgtgctgg cgccgatgct tgcgaacgtg ttggtgatgg cttggttgct gcgcacatca
     6961 ttgcgcgtgt ccactcagaa gtagaaaaca ttctgcctaa ggcgccacaa gcctaagtca
     7021 gatattccta agacggctca cttcggcgac acccgtcgcc gaaaccgtct aaccaaattt
     7081 ctgggctcgt tctgaacgtg ctcaacacta gttttagagg atctgttatg gcaacgactc
     7141 acggtattgc cctgggcatg attgaaacac gaggattggt tcctgccatt gaagccgcag
     7201 atgccatgac caaagcggcg gaagtccgtc tggtcggacg atcatttgtt ggcggcggtt
     7261 acgtgaccgt aatggttcgt ggtgagacag gtgcagtaaa tgctgccgtt cgtgcgggtg
     7321 ctgacgcctg tgaacgtgtt ggcgatggcc tggttgctgc gcacatcatt gcgcgcgttc
     7381 attctgaagt tgagatcatc ctacccgaga cgcccgaaga ctcagattcc gcgtggtgta
     7441 tcgcaaatct gaatagctaa tgtctagtag ggaagatgcg catgaacaac attgatttgc
     7501 gcgtctattc gtttatcgat tcgcttcaac cgcagcttgc atcctatctt gcgacatcat
     7561 cgcaaggctt tcttcccgtt ccgggtgatg cctgcttgtg gattgaagtc gcgccgggca
     7621 tggccgtcca tcgcctcagt gatattgcgc taaaggccac gaacgttcgt ctcggcgaac
     7681 aggtagtcga gcgtgctttt ggctcgatgg aaattcatta ccgaaaccaa agcgacgttc
     7741 tcgcatccgg tgaggccgtt ttaagagaaa tcaatcacgc gcaagaagat cgtctgcctt
     7801 gtcgcatcgc atggaaagag atcattcgag cgattacccc cgatcatgcc accttgatca
     7861 atcgccagtt gcgtaaaggc tctatgctgt tgccgggcaa aagcatgttc atccttgaaa
     7921 cagaaccggc aggttatatt gttcaggctg ccaacgaggc cgagaaagca gctcatgtga
     7981 ctctgatcga tgtacgtgct tttggtaact ttggtcgcct gaccatgatg ggcagcgaag
     8041 cggaaaccga agaagccatg cgggcggctg aggccacaat cgcaagcatc aacgcgcgtg
     8101 cgcgtcgcgc tgaagggttc taagcggccg cgaggttcca actttcacca taatgaaata
     8161 agatcactac cgggcgtatt ttttgagtta tcgagatttt caggagctaa ggaagctaaa
     8221 atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca tcgtaaagaa
     8281 cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt tcagctggat
     8341 attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc ggcctttatt
     8401 cacattcttg cccgcctgat gaatgctcat ccggagttcc gtatggcaat gaaagacggt
     8461 gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga gcaaactgaa
     8521 acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct acacatatat
     8581 tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg gtttattgag
     8641 aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga tttaaacgtg
     8701 gccaatatgg acaacttctt cgcccccgtt ttcactatgg gcaaatatta tacgcaaggc
     8761 gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga tggcttccat
     8821 gtcggcagaa tgcttaatga attacaacag tactgcgatg agtggcaggg cggggcgtaa
     8881 tttttttaag gcagttattg gtgcccttga attcctacta gtcgaagcgg catgcattta
     8941 cgttgacacc atcgaatggt gcaaaacctt tcgcggtatg gcatgatagc gcccggaaga
     9001 gagtcaattc agggtggtga atgtgaaacc agtaacgtta tacgatgtcg cagagtatgc
     9061 cggtgtctct tatcagaccg tttcccgcgt ggtgaaccag gccagccacg tttctgcgaa
     9121 aacgcgggaa aaagtggaag cggcgatggc ggagctgaat tacattccca accgcgtggc
     9181 acaacaactg gcgggcaaac agtcgttgct gattggcgtt gccacctcca gtctggccct
     9241 gcacgcgccg tcgcaaattg tcgcggcgat taaatctcgc gccgatcaac tgggtgccag
     9301 cgtggtggtg tcgatggtag aacgaagcgg cgtcgaagcc tgtaaagcgg cggtgcacaa
     9361 tcttctcgcg caacgcgtca gtgggctgat cattaactat ccgctggatg accaggatgc
     9421 cattgctgtg gaagctgcct gcactaatgt tccggcgtta tttcttgatg tctctgacca
     9481 gacacccatc aacagtatta ttttctccca tgaagacggt acgcgactgg gcgtggagca
     9541 tctggtcgca ttgggtcacc agcaaatcgc gctgttagcg ggcccattaa gttctgtctc
     9601 ggcgcgtctg cgtctggctg gctggcataa atatctcact cgcaatcaaa ttcagccgat
     9661 agcggaacgg gaaggcgact ggagtgccat gtccggtttt caacaaacca tgcaaatgct
     9721 gaatgagggc atcgttccca ctgcgatgct ggttgccaac gatcagatgg cgctgggcgc
     9781 aatgcgcgcc attaccgagt ccgggctgcg cgttggtgcg gatatctcgg tagtgggata
     9841 cgacgatacc gaagacagct catgttatat cccgccgtta accaccatca aacaggattt
     9901 tcgcctgctg gggcaaacca gcgtggaccg cttgctgcaa ctctctcagg gccaggcggt
     9961 gaagggcaat cagctgttgc ccgtctcact ggtgaaaaga aaaaccaccc tggcgcccaa
    10021 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
    10081 ttcccgactg gaaagcgggc agtgatccca cagccgccag ttccgctggc ggcattttaa
    10141 ctttctttaa taatctagtg acaagccggg gcagacgtga gccgtagtcc cgtcgccaga
    10201 cgcgggtgcc cacgggcgtc gtcaggatgt ccgtaattga ctgccggagg tggtcaatgc
    10261 ccttcagctc cttgccactg tcacggctca tgcctcgggt cattagtcgc ccgctccggt
    10321 atcttcactg gcttcgatga ttgccgcccc gcagctgcag aggtcaccga tccgagcagt
    10381 cggcctctgg ttggtaaaga ccgtgcgact gccggtgatg atcgtgttca agccatgcag
    10441 ggggcaggcg tgaaggtcat cctttcgggc cacggggcgg ctgttgacga aggtgtcgtc
    10501 gctcccggtg atgatgatcc cgccgtgatc ggtcacgtcg tttagtcgag cgatgcctgg
    10561 cgtcgtagtc acgggtttag gtcaatacga cttgcggtca ctgtaacgtt gccctcggcg
    10621 gtcacgttaa cgtcgccttg ggcttcgact tgcgcctcct gcacaaggat cacaatccgt
    10681 ccttgggctg cggtgaggtc gatcttgtac tcatgcgctt cgcggtcgta ctggatgatt
    10741 gagtcatcct cgaactgcgt cttttggatc gtttctttgt cctcgatctg ggggtagtca
    10801 gtcgagaacg cgccgggcat cgcgaagccc tgactgatct cgccggaggg ggccatcacg
    10861 acgacggcct caccgacctc gggcgcccac cagaaccgat ccttgcccgc tcgctgcgtg
    10921 agccacggaa tccagtcagt gaggagcagc gcctcgccgc tctcctcgtc ttcctcgatc
    10981 gcgacacgga tcagcccctt gggatagtca gcctcggcta ccctgcctac gcggagcaag
    11041 ttgccgtgac gccgactgtc tcgagtctag tcgcgttgct ggcgtttttc cataggctcc
    11101 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
    11161 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
    11221 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
    11281 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
    11341 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
    11401 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
    11461 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
    11521 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
    11581 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
    11641 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
    11701 ggtctgacgc tcagtctagt tcagccagct cgtcgtgatg tcgaaaccca agccaccctc
    11761 agaggtgaag gccgcttcga gcacatcggg aagcgtgtcg acgacggtgc tcggtgcctc
    11821 gggtaagagc cagatagatg cggtgacgtt caccgtcgcg atcgtggcgg gaaccacggt
    11881 cactgcgtcc gttacaactc gcacgtcgtc ggccagcacg actgactcga cggcttcgat
    11941 tagcccggga gaggcaaggc catcaggctc ggtagacaga atgctgatta acacctcgcc
    12001 gggagcaggg ctgctcacag ccgcgtcctt cacccgtggg tcagccgtca gcgcttgata
    12061 gcggtaccag gccgcaccgc ctgcggtcga gctgcccttg atccgctcga tggtgcgatc
    12121 gcgaagctcg ccatccggct cattcggctg ccgcaccacg ccgtagaacg cggcaaggtt
    12181 gtcgaggtcg gggccgtttg agtagcgaag cagtgtcgca agaagtgcgt cgttgatccg
    12241 ctgacgcagg atcagctcgc gggctgcgca gacctccagc agcttgatga ccgggtcgga
    12301 ttcgaggatg gcggtgtagc tggcgtcacg cgatcgcagg tcgtcgatca agtcctgcag
    12361 gatcagttca aagtccagcg cttcgatgat ggtgggcgcg ggaatcgtag caaagtcaag
    12421 aacggtcatg agacgactaa gccctccagc gtgatacgcc tgccctcggg gatgtagtag
    12481 ccgatcaggt tcagctcgac ttgaccagct gcgct
//
