LOCUS       sequence-300514-       12848 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Same as pLz37 but with CsoS2 discontinuously truncated such that 
            repeats N1, N2, and N4 are removed.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12848)
  AUTHORS   Oltrogge LM, Chaijarasphong T, Chen AW, Bolin ER, Marqusee S, Savage
            DF
  TITLE     Multivalent interactions between CsoS2 and Rubisco mediate 
            alpha-carboxysome formation.
  JOURNAL   Nat Struct Mol Biol. 2020 Mar;27(3):281-287. doi: 
            10.1038/s41594-020-0387-7. Epub 2020 Mar 2.
  PUBMED    32123388
REFERENCE   2  (bases 1 to 12848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41594-020-0387-7"; journalName: "Nat Struct Mol Biol"; 
            date: "2020-03"; volume: "27"; issue: "3"; pages: "281-287"
COMMENT     Sequence Label: pLz77_CB_operon_CsoS2_N3
FEATURES             Location/Qualifiers
     source          1..12848
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      119..205
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      297..324
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     protein_bind    346..365
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     promoter        join(380..385,386..403,404..410)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    418..434
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             8559..9218
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(8625..8644)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        9283..9360
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             9361..10443
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(9380..9399)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     rep_origin      complement(11425..12013)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11505..11524)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcgctggctg agacgatgcg aaccttctcc agcttcagcc gtggctccca gcgatcgagc
       61 gcttcagctg tggccgccac caggtcaacg atgagggact ggttgatcgg ctagtcatca
      121 aataaaacga aaggctcagt cggaagactg ggcctttcgt tttatctgtt gtttgtcggt
      181 gaacgctctc ctgagtagga caaatccgcc gggagcggat ttgaacgttg cgaagcaacg
      241 gcccggaggg tggcgggcag gacgcccgcc ataaactgcc aggcatcaaa ttaagcagaa
      301 ggccatcctg acggatggcc tttttgcgtt tctactctag tttcgaattg tgagcgctca
      361 caatttcgaa accccaggct tgacacttta tgcttccggc tcgtataatg tgtggaattg
      421 tgagcggata acaatttcag ctaggtgcgc gaatccccca tcctttcagg aggaactcat
      481 ggcagttaaa aagtatagtg ctggtgtaaa agaataccgg cagacctatt ggatgccgga
      541 atacacaccg ttggattccg acatccttgc atgcttcaaa atcaccccac aaccgggtgt
      601 tgatcgcgaa gaagccgcag ccgcggttgc agcagaatct tcaaccggca catggaccac
      661 cgtgtggacc gatttgctga ccgacatgga ctactacaaa ggccgtgcct atcgcattga
      721 agacgtaccc ggtgacgatg cggcattcta tgcctttatc gcctacccaa tcgacctgtt
      781 cgaagaaggg tcagttgtta acgtgtttac ctcactggtt ggtaacgtgt tcggcttcaa
      841 agcggtacgc ggcctgcgtc tggaagatgt tcgcttccca ctcgcctacg ttaaaacctg
      901 tggcggccca ccgcacggta ttcaagtcga acgcgacaag atgaacaaat atggtcgccc
      961 actgttgggt tgcaccatca agccaaaact tggtttgtct gcgaaaaact acggccgtgc
     1021 cgtatacgag tgcctccgtg gcggcctcga cttcactaaa gatgatgaaa acatcaattc
     1081 tcagccgttc atgcgctggc gcgatcgctt cttgttcgta caagacgcga ccgaaactgc
     1141 tgaagcccaa accggcgaac gcaaaggcca ttacctcaac gtaacggcgc caactcctga
     1201 agaaatgtac aagcgcgcag aattcgccaa agaaattggc gcgccaatca ttatgcacga
     1261 ctacatcacc ggtggcttca cggccaacac tggcttggcc aagtggtgtc aagacaacgg
     1321 cgtactgctg cacatccacc gtgcgatgca tgcggttatc gaccgtaacc cgaaccacgg
     1381 tattcacttc cgtgttctga ccaagattct gcgtttgtcg ggtggcgatc acctgcacac
     1441 cggtaccgtt gtcggcaaac tggaaggcga ccgtgcctct actctgggct ggattgattt
     1501 gctccgcgaa tcgtttatcc ctgaagatcg ctcgcgcggt atcttcttcg atcaagactg
     1561 gggttcaatg ccaggcgtat tcgctgtggc ctctggtggt attcacgtat ggcacatgcc
     1621 tgcgctggtt aacatctttg gtgacgactc tgtcctccaa ttcggtggcg gtacgctggg
     1681 tcatccatgg ggcaacgctg ccggtgctgc tgccaaccgt gttgctctgg aagcctgcgt
     1741 agaagcgcgt aaccaaggcc gcgatatcga aaaagaaggc aaagaaattc tgactgctgc
     1801 tgcacagcac agcccagaac tgaagattgc catggaaact tggaaagaga tcaaattcga
     1861 atttgacact gtcgacaaac tcgacactca aaatcgttga tccctcgtac cacacaacat
     1921 actaaggtga gtaaccatgg ctgaaatgca ggattacaag caaagcctca aatatgagac
     1981 tttctcttat cttccaccca tgaacgcgga acgcatccgc gctcaaatca agtacgcaat
     2041 tgctcaaggc tggagccccg gcattgagca cgtagaagtg aaaaactcca tgaaccaata
     2101 ttggtacatg tggaaacttc ccttcttcgg cgaacaaaat gtcgacaacg tgttggctga
     2161 aattgaagcg tgtcgtagtg cgtatccaac acaccaggtc aaactggtgg cttatgacaa
     2221 ctatgcgcaa agcttaggtc tggccttcgt ggtctaccgc ggcaactaag tcaagtctgt
     2281 cattgcgctg tgcgtttctc tacgcacagc acttattcaa gatggggtca aacatgaaag
     2341 cggttaaaac caacggcaag ccgtcgggtc gcgttcgacc acagcaatca gtggttcaca
     2401 acgacgcaat gatcgaaaat gccggtgata ctaaccaatc atcgtccact tcattaaata
     2461 atgaactttc cgaaatctgc tccatagcag acgacatgcc ggagcgtttt ggttcacaag
     2521 ccaaaaccgt ccgtgatatc tgccgtgcac gccgtcaagc gctctctgag cgtggaactc
     2581 gcgccgtgcc gccaaagccg caatctcaag gtggtccagg acgcaatggc tatcaaattg
     2641 atggatacct agataccgca cttcatggcg gcacagcacc ttcctgcaag ccaacaggcc
     2701 gtgtcaaaaa ttctgtacag tcgggcaacg cagcgccaaa aaaggttgaa accggtcaca
     2761 ccctatccgg cggatctgtt acgggcacgc aagtggatcg taaatctcat gtgactggaa
     2821 acgagccggg cacttgccga gcagtcacgg gcaccgagta cgtaggtact gagcaattca
     2881 cctctttttg caataccagc cccaagccaa atgcgacgaa ggtcaatgtg accacaacgg
     2941 ctcgtggtcg ccctgttagc ggtacggaag tttcacggac cgagaaggta actggcaacg
     3001 aatccggtgt ctgccgtaac gttaccggca ccgaatacat gagtaatgaa gctcactttt
     3061 ctctatgtgg aacagccgca aagccttcac aagcggataa agtcatgttc ggcgccacag
     3121 cacgaacgca tcaagtggtc agtggcagtg atgaattcag gccctcttct gttacgggta
     3181 acgaatcggg tgcaaaacgc acaattaccg gctcgcagta cgcagacgaa ggtcttgcgc
     3241 gactcacgat caacggagca cctgcaaaag tagccagaac ccacaccttt gcgggctctg
     3301 acgttaccgg cacggaaatc ggtcgctcta ctcgcgtaac tggtgatgaa agcggttcgt
     3361 gtcgttcaat ctcaggcacc gagtatctca gtaacgagca attccaatcc ttttgtgaca
     3421 caaaacctca acgcagcccg ttcaaggttg gccaagatcg cacgaacaag ggtcagtctg
     3481 tgactggtaa cttggttgat cgttccgaac tggttacagg taacgaacca ggttcatgct
     3541 ctcgggttac aggctctcag tatggccaaa gcaaaatctg cggtggtggc gtgggaaaag
     3601 tgcgctcaat gcgcaccctt cgcggcacct cagtatctgg ccaacagcta gatcatgccc
     3661 caaagatgtc cggtgacgag cgcggcgggt gcatgcccgt caccggtaat gagtactacg
     3721 gtcgtgaaca tttcgaaccg ttttgtacga gcaccccaga gcccgaagct caatcaactg
     3781 aacaatcatt gacctgtgaa ggacaaatta ttagcggcac ttcagttgac gccagtgatt
     3841 tggtcacagg aaatgaaatc ggtgaacagc aactcatcag cggtgacgcc tatgttggcg
     3901 cgcagcagac aggttgcctt cccactagtc cacgcttcaa ccaaactggc aatgttcagt
     3961 caatgggttt taagaacacc aatcagccag aacaaaactt tgcaccaggt gaagtaatgc
     4021 ctactgactt tagtattcaa accccagctc gctcggctca gaatcgcatt acaggtaacg
     4081 acattgcgcc ctcaggtcgc attacaggcc ctggtatgct ggcaaccggc ttgattacag
     4141 gaacccccga attcaggcac gctgcgcgcg agttggttgg ttctccacaa cccatggcaa
     4201 tggccatggc caaccgtaat aaggctgctc aagcacctgt tgtgcagcca gaagtggttg
     4261 caactcagga aaagcctgag ttggtatgtg caccaagaag cgatcaaatg gatcgtgtga
     4321 gtggcgaagg caaagaacgt tgccacatca ctggcgatga ctggtcagtt aacaagcaca
     4381 tcaccggtac agccggtcaa tgggcgagtg gtcgcaaccc ttccatgcgc ggtaatgcgc
     4441 gtgtggtcga aaccagcgcg tttgccaatc gcaatgtgcc aaaacctgaa aagccgggct
     4501 ccaagatcac gggcagtagt ggtaatgaca cccaaggtag tctgatcact tactccggcg
     4561 gcgcgcgcgg ttgagcgtta agtaaagtgt aacgatcatg aacacccgta acacacgaag
     4621 caagcaacgc gcaccgtttg gtgttagctc atcagtcaaa cctcggcttg acttgattga
     4681 gcaagcacca aaccctgcct atgaccgcca tcctgcttgc ataacgttgc ctgagcgtac
     4741 ctgccggcac ccgttaactg accttgaagc caacgaacaa ctgggtcgtt gcgaggatag
     4801 cgtcaagaac cgttttgatc gcgttatccc tttcttgcaa gttgttgctg gcattcctct
     4861 tggtctggat tatgttaccc gtgttcaaga attagcccag tcgtcgcttg gacatacgct
     4921 gcccgaagaa ttactcaaag ataattggat cagtgggcac aatttaaaag gcatttttgg
     4981 ctacgcgact gctaaagcac taacagcagc cacggaacaa ttcagtcgta aaataatgtc
     5041 tgagaaggac gattccgcat cggccattgg cttcttcctg gattgcgggt tccacgcagt
     5101 ggacataagc ccttgtgccg atggtcgtct caaagggtta ctgccttaca tattgcgttt
     5161 gccccttacg gcattcacct atcgtaaagc ctacgcaggc tcgatgttcg atattgaaga
     5221 tgatctggca cagtgggaga aaaatgaact ccgccgttat cgtgaaggtg ttccaaatac
     5281 agcggatcag ccaacacgat acctgaaaat tgctgtgtat catttcagca cctctgaccc
     5341 gacacactct ggctgcgcgg cacacggcag taatgatcgt gcagcactgg aagcggcttt
     5401 aacccagctg atgaaattca gagaagcggt tgaaaatgcc cattgctgcg gcgcaagtat
     5461 cgatatttta ctgattggcg ttgatacgga tacggatgcc attcgcgttc atattccgga
     5521 tagcaaaggt tttttgaatc cgtatcgtta tgttgacaac acagtaactt atgcgcaaac
     5581 actacatctg gcgccggatg aggctcgtgt gattattcac gaagcaattc tcaacgcaaa
     5641 ccgcagcgat ggttgggcta aaggaaatgg agtagccagc gaggggatgc gtcgttttat
     5701 tggtcagctt ttgatcaaca acctctcgca aatcgattac gtagtaaatc gtcatggtgg
     5761 tcgctatcca cccaatgata ttggtcatgc tgagcgatat atcagtgttg gtgatggttt
     5821 tgatgaagtt caaatccgga atttagccta ctacgcgcat ttggatacgg ttgaagaaaa
     5881 tgcgattgat gtggatgtgg gaatcaaaat tttcaccaaa cttaatttga gtcgaggttt
     5941 accgattccg attgccatcc actatcgcta tgaccccaat gttccaggct ccagagaaag
     6001 aaccgtggtc aaagcaagac ggatatacaa cgccattaaa gagcggttct catccttgga
     6061 tgagcagaat ctattgcagt ttcgtttgag cgttcaggcg caggatatcg gaagcccgat
     6121 tgaagaggtt gcatccgcat gaaaatcatg caagttgaga aaacgttggt ttcaacaaac
     6181 cgtattgctg atatgggtca caaaccacta ttagtggtat gggagaaacc gggcgcgccc
     6241 aggcaggtcg ccgtggatgc gattggctgc ataccgggcg attgggtttt gtgcgttggg
     6301 tcatcggcag cacgagaggc tgcaggaagc aagtcttatc cctctgattt gacgattatc
     6361 gggattattg atcagtggaa tggtgagtaa tggaagtaat gcgcgttcgt tccgacctaa
     6421 tcgcaacacg caggattccc ggtcttaaaa atatctcttt gcgtgtcatg gaggatgcta
     6481 cgggtaaggt cagtgtcgct tgcgatccca ttggcgttcc cgagggatgt tgggtcttta
     6541 cgattagcgg ctctgccgct cggtttggcg tgggtgattt tgagattctc acggatttga
     6601 cgattggtgg catcatcgat cactgggtaa cttgaccatc gctagatgag ttgattttga
     6661 atgagtcttt attgaggaga gaagaaatgg cagcagtaac aggtattgca ctgggtatga
     6721 ttgaaacacg tggtctggtt ccagcgattg aagctgccga tgccatgacc aaggccgccg
     6781 aagtacgttt ggttggccgt caatttgttg gtggtggtta cgtgaccgtt ttggtccgtg
     6841 gtgaaaccgg tgccgtcaac gcagcagttc gtgcgggcgc tgatgcctgc gaacgagtcg
     6901 gcgatggtct ggtcgcggcg catatcattg cccgtgtcca ttccgaagtc gaaaacatcc
     6961 tgccgaaagc ccctgaagct taaggattgg gaaagacgaa ccggcgcagg cttgttccgg
     7021 ttctttgcat aaagtgacag cttaggagtt tatttaaatg gctgatgtaa ctggtattgc
     7081 tctgggtatg atcgaaacac gtggcttagt tcctgcgatt gaagcagcgg acgccatgac
     7141 taaagcggct gaagtgcgtt tggtcggtcg tcaatttgtt ggtggcggtt acgtcaccgt
     7201 attggttcgg ggcgaaacag gcgctgttaa cgccgctgtt cgtgctggcg ccgatgcttg
     7261 cgaacgtgtt ggtgatggct tggttgctgc gcacatcatt gcgcgtgtcc actcagaagt
     7321 agaaaacatt ctgcctaagg cgccacaagc ctaagtcaga tattcctaag acggctcact
     7381 tcggcgacac ccgtcgccga aaccgtctaa ccaaatttct gggctcgttc tgaacgtgct
     7441 caacactagt tttagaggat ctgttatggc aacgactcac ggtattgccc tgggcatgat
     7501 tgaaacacga ggattggttc ctgccattga agccgcagat gccatgacca aagcggcgga
     7561 agtccgtctg gtcggacgat catttgttgg cggcggttac gtgaccgtaa tggttcgtgg
     7621 tgagacaggt gcagtaaatg ctgccgttcg tgcgggtgct gacgcctgtg aacgtgttgg
     7681 cgatggcctg gttgctgcgc acatcattgc gcgcgttcat tctgaagttg agatcatcct
     7741 acccgagacg cccgaagact cagattccgc gtggtgtatc gcaaatctga atagctaatg
     7801 tctagtaggg aagatgcgca tgaacaacat tgatttgcgc gtctattcgt ttatcgattc
     7861 gcttcaaccg cagcttgcat cctatcttgc gacatcatcg caaggctttc ttcccgttcc
     7921 gggtgatgcc tgcttgtgga ttgaagtcgc gccgggcatg gccgtccatc gcctcagtga
     7981 tattgcgcta aaggccacga acgttcgtct cggcgaacag gtagtcgagc gtgcttttgg
     8041 ctcgatggaa attcattacc gaaaccaaag cgacgttctc gcatccggtg aggccgtttt
     8101 aagagaaatc aatcacgcgc aagaagatcg tctgccttgt cgcatcgcat ggaaagagat
     8161 cattcgagcg attacccccg atcatgccac cttgatcaat cgccagttgc gtaaaggctc
     8221 tatgctgttg ccgggcaaaa gcatgttcat ccttgaaaca gaaccggcag gttatattgt
     8281 tcaggctgcc aacgaggccg agaaagcagc tcatgtgact ctgatcgatg tacgtgcttt
     8341 tggtaacttt ggtcgcctga ccatgatggg cagcgaagcg gaaaccgaag aagccatgcg
     8401 ggcggctgag gccacaatcg caagcatcaa cgcgcgtgcg cgtcgcgctg aagggttcta
     8461 agcggccgcg aggttccaac tttcaccata atgaaataag atcactaccg ggcgtatttt
     8521 ttgagttatc gagattttca ggagctaagg aagctaaaat ggagaaaaaa atcactggat
     8581 ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca tttcagtcag
     8641 ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt ttaaagaccg
     8701 taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc cgcctgatga
     8761 atgctcatcc ggagttccgt atggcaatga aagacggtga gctggtgata tgggatagtg
     8821 ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatcg ctctggagtg
     8881 aataccacga cgatttccgg cagtttctac acatatattc gcaagatgtg gcgtgttacg
     8941 gtgaaaacct ggcctatttc cctaaagggt ttattgagaa tatgtttttc gtctcagcca
     9001 atccctgggt gagtttcacc agttttgatt taaacgtggc caatatggac aacttcttcg
     9061 cccccgtttt cactatgggc aaatattata cgcaaggcga caaggtgctg atgccgctgg
     9121 cgattcaggt tcatcatgcc gtttgtgatg gcttccatgt cggcagaatg cttaatgaat
     9181 tacaacagta ctgcgatgag tggcagggcg gggcgtaatt tttttaaggc agttattggt
     9241 gcccttgaat tcctactagt cgaagcggca tgcatttacg ttgacaccat cgaatggtgc
     9301 aaaacctttc gcggtatggc atgatagcgc ccggaagaga gtcaattcag ggtggtgaat
     9361 gtgaaaccag taacgttata cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt
     9421 tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg
     9481 gcgatggcgg agctgaatta cattcccaac cgcgtggcac aacaactggc gggcaaacag
     9541 tcgttgctga ttggcgttgc cacctccagt ctggccctgc acgcgccgtc gcaaattgtc
     9601 gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa
     9661 cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt
     9721 gggctgatca ttaactatcc gctggatgac caggatgcca ttgctgtgga agctgcctgc
     9781 actaatgttc cggcgttatt tcttgatgtc tctgaccaga cacccatcaa cagtattatt
     9841 ttctcccatg aagacggtac gcgactgggc gtggagcatc tggtcgcatt gggtcaccag
     9901 caaatcgcgc tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg tctggctggc
     9961 tggcataaat atctcactcg caatcaaatt cagccgatag cggaacggga aggcgactgg
    10021 agtgccatgt ccggttttca acaaaccatg caaatgctga atgagggcat cgttcccact
    10081 gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc
    10141 gggctgcgcg ttggtgcgga tatctcggta gtgggatacg acgataccga agacagctca
    10201 tgttatatcc cgccgttaac caccatcaaa caggattttc gcctgctggg gcaaaccagc
    10261 gtggaccgct tgctgcaact ctctcagggc caggcggtga agggcaatca gctgttgccc
    10321 gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc
    10381 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
    10441 tgatcccaca gccgccagtt ccgctggcgg cattttaact ttctttaata atctagtgac
    10501 aagccggggc agacgtgagc cgtagtcccg tcgccagacg cgggtgccca cgggcgtcgt
    10561 caggatgtcc gtaattgact gccggaggtg gtcaatgccc ttcagctcct tgccactgtc
    10621 acggctcatg cctcgggtca ttagtcgccc gctccggtat cttcactggc ttcgatgatt
    10681 gccgccccgc agctgcagag gtcaccgatc cgagcagtcg gcctctggtt ggtaaagacc
    10741 gtgcgactgc cggtgatgat cgtgttcaag ccatgcaggg ggcaggcgtg aaggtcatcc
    10801 tttcgggcca cggggcggct gttgacgaag gtgtcgtcgc tcccggtgat gatgatcccg
    10861 ccgtgatcgg tcacgtcgtt tagtcgagcg atgcctggcg tcgtagtcac gggtttaggt
    10921 caatacgact tgcggtcact gtaacgttgc cctcggcggt cacgttaacg tcgccttggg
    10981 cttcgacttg cgcctcctgc acaaggatca caatccgtcc ttgggctgcg gtgaggtcga
    11041 tcttgtactc atgcgcttcg cggtcgtact ggatgattga gtcatcctcg aactgcgtct
    11101 tttggatcgt ttctttgtcc tcgatctggg ggtagtcagt cgagaacgcg ccgggcatcg
    11161 cgaagccctg actgatctcg ccggaggggg ccatcacgac gacggcctca ccgacctcgg
    11221 gcgcccacca gaaccgatcc ttgcccgctc gctgcgtgag ccacggaatc cagtcagtga
    11281 ggagcagcgc ctcgccgctc tcctcgtctt cctcgatcgc gacacggatc agccccttgg
    11341 gatagtcagc ctcggctacc ctgcctacgc ggagcaagtt gccgtgacgc cgactgtctc
    11401 gagtctagtc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
    11461 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
    11521 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
    11581 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
    11641 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
    11701 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
    11761 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
    11821 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
    11881 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
    11941 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
    12001 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtctagttc
    12061 agccagctcg tcgtgatgtc gaaacccaag ccaccctcag aggtgaaggc cgcttcgagc
    12121 acatcgggaa gcgtgtcgac gacggtgctc ggtgcctcgg gtaagagcca gatagatgcg
    12181 gtgacgttca ccgtcgcgat cgtggcggga accacggtca ctgcgtccgt tacaactcgc
    12241 acgtcgtcgg ccagcacgac tgactcgacg gcttcgatta gcccgggaga ggcaaggcca
    12301 tcaggctcgg tagacagaat gctgattaac acctcgccgg gagcagggct gctcacagcc
    12361 gcgtccttca cccgtgggtc agccgtcagc gcttgatagc ggtaccaggc cgcaccgcct
    12421 gcggtcgagc tgcccttgat ccgctcgatg gtgcgatcgc gaagctcgcc atccggctca
    12481 ttcggctgcc gcaccacgcc gtagaacgcg gcaaggttgt cgaggtcggg gccgtttgag
    12541 tagcgaagca gtgtcgcaag aagtgcgtcg ttgatccgct gacgcaggat cagctcgcgg
    12601 gctgcgcaga cctccagcag cttgatgacc gggtcggatt cgaggatggc ggtgtagctg
    12661 gcgtcacgcg atcgcaggtc gtcgatcaag tcctgcagga tcagttcaaa gtccagcgct
    12721 tcgatgatgg tgggcgcggg aatcgtagca aagtcaagaa cggtcatgag acgactaagc
    12781 cctccagcgt gatacgcctg ccctcgggga tgtagtagcc gatcaggttc agctcgactt
    12841 gaccagct
//
