LOCUS       sequence-300515-       12896 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Same as pLz37 but with CsoS2 truncated such that NTD repeats N1 and 
            N2 are removed.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12896)
  AUTHORS   Oltrogge LM, Chaijarasphong T, Chen AW, Bolin ER, Marqusee S, Savage
            DF
  TITLE     Multivalent interactions between CsoS2 and Rubisco mediate 
            alpha-carboxysome formation.
  JOURNAL   Nat Struct Mol Biol. 2020 Mar;27(3):281-287. doi: 
            10.1038/s41594-020-0387-7. Epub 2020 Mar 2.
  PUBMED    32123388
REFERENCE   2  (bases 1 to 12896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41594-020-0387-7"; journalName: "Nat Struct Mol Biol"; 
            date: "2020-03"; volume: "27"; issue: "3"; pages: "281-287"
COMMENT     Sequence Label: pLz75_CB_operon_CsoS2_N3-N4
FEATURES             Location/Qualifiers
     source          1..12896
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      152..238
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      330..357
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     protein_bind    379..398
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     promoter        join(413..418,419..436,437..443)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    451..467
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             8640..9299
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(8706..8725)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        9364..9441
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             9442..10524
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(9461..9480)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     rep_origin      complement(11506..12094)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11586..11605)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagccgatca ggttcagctc gacttgacca gctgcgctgg ctgagacgat gcgaaccttc
       61 tccagcttca gccgtggctc ccagcgatcg agcgcttcag ctgtggccgc caccaggtca
      121 acgatgaggg actggttgat cggctagtca tcaaataaaa cgaaaggctc agtcggaaga
      181 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc
      241 gccgggagcg gatttgaacg ttgcgaagca acggcccgga gggtggcggg caggacgccc
      301 gccataaact gccaggcatc aaattaagca gaaggccatc ctgacggatg gcctttttgc
      361 gtttctactc tagtttcgaa ttgtgagcgc tcacaatttc gaaaccccag gcttgacact
      421 ttatgcttcc ggctcgtata atgtgtggaa ttgtgagcgg ataacaattt cagctaggtg
      481 cgcgaatccc ccatcctttc aggaggaact catggcagtt aaaaagtata gtgctggtgt
      541 aaaagaatac cggcagacct attggatgcc ggaatacaca ccgttggatt ccgacatcct
      601 tgcatgcttc aaaatcaccc cacaaccggg tgttgatcgc gaagaagccg cagccgcggt
      661 tgcagcagaa tcttcaaccg gcacatggac caccgtgtgg accgatttgc tgaccgacat
      721 ggactactac aaaggccgtg cctatcgcat tgaagacgta cccggtgacg atgcggcatt
      781 ctatgccttt atcgcctacc caatcgacct gttcgaagaa gggtcagttg ttaacgtgtt
      841 tacctcactg gttggtaacg tgttcggctt caaagcggta cgcggcctgc gtctggaaga
      901 tgttcgcttc ccactcgcct acgttaaaac ctgtggcggc ccaccgcacg gtattcaagt
      961 cgaacgcgac aagatgaaca aatatggtcg cccactgttg ggttgcacca tcaagccaaa
     1021 acttggtttg tctgcgaaaa actacggccg tgccgtatac gagtgcctcc gtggcggcct
     1081 cgacttcact aaagatgatg aaaacatcaa ttctcagccg ttcatgcgct ggcgcgatcg
     1141 cttcttgttc gtacaagacg cgaccgaaac tgctgaagcc caaaccggcg aacgcaaagg
     1201 ccattacctc aacgtaacgg cgccaactcc tgaagaaatg tacaagcgcg cagaattcgc
     1261 caaagaaatt ggcgcgccaa tcattatgca cgactacatc accggtggct tcacggccaa
     1321 cactggcttg gccaagtggt gtcaagacaa cggcgtactg ctgcacatcc accgtgcgat
     1381 gcatgcggtt atcgaccgta acccgaacca cggtattcac ttccgtgttc tgaccaagat
     1441 tctgcgtttg tcgggtggcg atcacctgca caccggtacc gttgtcggca aactggaagg
     1501 cgaccgtgcc tctactctgg gctggattga tttgctccgc gaatcgttta tccctgaaga
     1561 tcgctcgcgc ggtatcttct tcgatcaaga ctggggttca atgccaggcg tattcgctgt
     1621 ggcctctggt ggtattcacg tatggcacat gcctgcgctg gttaacatct ttggtgacga
     1681 ctctgtcctc caattcggtg gcggtacgct gggtcatcca tggggcaacg ctgccggtgc
     1741 tgctgccaac cgtgttgctc tggaagcctg cgtagaagcg cgtaaccaag gccgcgatat
     1801 cgaaaaagaa ggcaaagaaa ttctgactgc tgctgcacag cacagcccag aactgaagat
     1861 tgccatggaa acttggaaag agatcaaatt cgaatttgac actgtcgaca aactcgacac
     1921 tcaaaatcgt tgatccctcg taccacacaa catactaagg tgagtaacca tggctgaaat
     1981 gcaggattac aagcaaagcc tcaaatatga gactttctct tatcttccac ccatgaacgc
     2041 ggaacgcatc cgcgctcaaa tcaagtacgc aattgctcaa ggctggagcc ccggcattga
     2101 gcacgtagaa gtgaaaaact ccatgaacca atattggtac atgtggaaac ttcccttctt
     2161 cggcgaacaa aatgtcgaca acgtgttggc tgaaattgaa gcgtgtcgta gtgcgtatcc
     2221 aacacaccag gtcaaactgg tggcttatga caactatgcg caaagcttag gtctggcctt
     2281 cgtggtctac cgcggcaact aagtcaagtc tgtcattgcg ctgtgcgttt ctctacgcac
     2341 agcacttatt caagatgggg tcaaacatga aagcggttaa aaccaacggc aagccgtcgg
     2401 gtcgcgttcg accacagcaa tcagtggttc acaacgacgc aatgatcgaa aatgccggtg
     2461 atactaacca atcatcgtcc acttcattaa ataatgaact ttccgaaatc tgctccatag
     2521 cagacgacat gccggagcgt tttggttcac aagccaaaac cgtccgtgat atctgccgtg
     2581 cacgccgtca agcgctctct gagcgtggaa ctcgcgccgt gccgccaaag ccgcaatctc
     2641 aaggtggtcc aggacgcaat ggctatcaaa ttgatggata cctagatacc gcacttcatg
     2701 gccgcgatgc cgccaagcgc caccgtgaaa tgctctgtca atacggccgc ggcacagcac
     2761 cttcctgcaa gccaacaggc cgtgtcaaaa attctgtaca gtcgggcaac gcagcgccaa
     2821 aaaaggttga aaccggtcac accctatccg gcggatctgt tacgggcacg caagtggatc
     2881 gtaaatctca tgtgactgga aacgagccgg gcacttgccg agcagtcacg ggcaccgagt
     2941 acgtaggtac tgagcaattc acctcttttt gcaataccag ccccaagcca aatgcgacga
     3001 aggtcaatgt gaccacaacg gctcgtggtc gccctgttag cggtacggaa gtttcacgga
     3061 ccgagaaggt aactggcaac gaatccggtg tctgccgtaa cgttaccggc accgaataca
     3121 tgagtaatga agctcacttt tctctatgtg gaacagccgc aaagccttca caagcggata
     3181 aagtcatgtt cggcgccaca gcacgaacgc atcaagtggt cagtggcagt gatgaattca
     3241 ggccctcttc tgttacgggt aacgaatcgg gtgcaaaacg cacaattacc ggctcgcagt
     3301 acgcagacga aggtcttgcg cgactcacga tcaacggagc acctgcaaaa gtagccagaa
     3361 cccacacctt tgcgggctct gacgttaccg gcacggaaat cggtcgctct actcgcgtaa
     3421 ctggtgatga aagcggttcg tgtcgttcaa tctcaggcac cgagtatctc agtaacgagc
     3481 aattccaatc cttttgtgac acaaaacctc aacgcagccc gttcaaggtt ggccaagatc
     3541 gcacgaacaa gggtcagtct gtgactggta acttggttga tcgttccgaa ctggttacag
     3601 gtaacgaacc aggttcatgc tctcgggtta caggctctca gtatggccaa agcaaaatct
     3661 gcggtggtgg cgtgggaaaa gtgcgctcaa tgcgcaccct tcgcggcacc tcagtatctg
     3721 gccaacagct agatcatgcc ccaaagatgt ccggtgacga gcgcggcggg tgcatgcccg
     3781 tcaccggtaa tgagtactac ggtcgtgaac atttcgaacc gttttgtacg agcaccccag
     3841 agcccgaagc tcaatcaact gaacaatcat tgacctgtga aggacaaatt attagcggca
     3901 cttcagttga cgccagtgat ttggtcacag gaaatgaaat cggtgaacag caactcatca
     3961 gcggtgacgc ctatgttggc gcgcagcaga caggttgcct tcccactagt ccacgcttca
     4021 accaaactgg caatgttcag tcaatgggtt ttaagaacac caatcagcca gaacaaaact
     4081 ttgcaccagg tgaagtaatg cctactgact ttagtattca aaccccagct cgctcggctc
     4141 agaatcgcat tacaggtaac gacattgcgc cctcaggtcg cattacaggc cctggtatgc
     4201 tggcaaccgg cttgattaca ggaacccccg aattcaggca cgctgcgcgc gagttggttg
     4261 gttctccaca acccatggca atggccatgg ccaaccgtaa taaggctgct caagcacctg
     4321 ttgtgcagcc agaagtggtt gcaactcagg aaaagcctga gttggtatgt gcaccaagaa
     4381 gcgatcaaat ggatcgtgtg agtggcgaag gcaaagaacg ttgccacatc actggcgatg
     4441 actggtcagt taacaagcac atcaccggta cagccggtca atgggcgagt ggtcgcaacc
     4501 cttccatgcg cggtaatgcg cgtgtggtcg aaaccagcgc gtttgccaat cgcaatgtgc
     4561 caaaacctga aaagccgggc tccaagatca cgggcagtag tggtaatgac acccaaggta
     4621 gtctgatcac ttactccggc ggcgcgcgcg gttgagcgtt aagtaaagtg taacgatcat
     4681 gaacacccgt aacacacgaa gcaagcaacg cgcaccgttt ggtgttagct catcagtcaa
     4741 acctcggctt gacttgattg agcaagcacc aaaccctgcc tatgaccgcc atcctgcttg
     4801 cataacgttg cctgagcgta cctgccggca cccgttaact gaccttgaag ccaacgaaca
     4861 actgggtcgt tgcgaggata gcgtcaagaa ccgttttgat cgcgttatcc ctttcttgca
     4921 agttgttgct ggcattcctc ttggtctgga ttatgttacc cgtgttcaag aattagccca
     4981 gtcgtcgctt ggacatacgc tgcccgaaga attactcaaa gataattgga tcagtgggca
     5041 caatttaaaa ggcatttttg gctacgcgac tgctaaagca ctaacagcag ccacggaaca
     5101 attcagtcgt aaaataatgt ctgagaagga cgattccgca tcggccattg gcttcttcct
     5161 ggattgcggg ttccacgcag tggacataag cccttgtgcc gatggtcgtc tcaaagggtt
     5221 actgccttac atattgcgtt tgccccttac ggcattcacc tatcgtaaag cctacgcagg
     5281 ctcgatgttc gatattgaag atgatctggc acagtgggag aaaaatgaac tccgccgtta
     5341 tcgtgaaggt gttccaaata cagcggatca gccaacacga tacctgaaaa ttgctgtgta
     5401 tcatttcagc acctctgacc cgacacactc tggctgcgcg gcacacggca gtaatgatcg
     5461 tgcagcactg gaagcggctt taacccagct gatgaaattc agagaagcgg ttgaaaatgc
     5521 ccattgctgc ggcgcaagta tcgatatttt actgattggc gttgatacgg atacggatgc
     5581 cattcgcgtt catattccgg atagcaaagg ttttttgaat ccgtatcgtt atgttgacaa
     5641 cacagtaact tatgcgcaaa cactacatct ggcgccggat gaggctcgtg tgattattca
     5701 cgaagcaatt ctcaacgcaa accgcagcga tggttgggct aaaggaaatg gagtagccag
     5761 cgaggggatg cgtcgtttta ttggtcagct tttgatcaac aacctctcgc aaatcgatta
     5821 cgtagtaaat cgtcatggtg gtcgctatcc acccaatgat attggtcatg ctgagcgata
     5881 tatcagtgtt ggtgatggtt ttgatgaagt tcaaatccgg aatttagcct actacgcgca
     5941 tttggatacg gttgaagaaa atgcgattga tgtggatgtg ggaatcaaaa ttttcaccaa
     6001 acttaatttg agtcgaggtt taccgattcc gattgccatc cactatcgct atgaccccaa
     6061 tgttccaggc tccagagaaa gaaccgtggt caaagcaaga cggatataca acgccattaa
     6121 agagcggttc tcatccttgg atgagcagaa tctattgcag tttcgtttga gcgttcaggc
     6181 gcaggatatc ggaagcccga ttgaagaggt tgcatccgca tgaaaatcat gcaagttgag
     6241 aaaacgttgg tttcaacaaa ccgtattgct gatatgggtc acaaaccact attagtggta
     6301 tgggagaaac cgggcgcgcc caggcaggtc gccgtggatg cgattggctg cataccgggc
     6361 gattgggttt tgtgcgttgg gtcatcggca gcacgagagg ctgcaggaag caagtcttat
     6421 ccctctgatt tgacgattat cgggattatt gatcagtgga atggtgagta atggaagtaa
     6481 tgcgcgttcg ttccgaccta atcgcaacac gcaggattcc cggtcttaaa aatatctctt
     6541 tgcgtgtcat ggaggatgct acgggtaagg tcagtgtcgc ttgcgatccc attggcgttc
     6601 ccgagggatg ttgggtcttt acgattagcg gctctgccgc tcggtttggc gtgggtgatt
     6661 ttgagattct cacggatttg acgattggtg gcatcatcga tcactgggta acttgaccat
     6721 cgctagatga gttgattttg aatgagtctt tattgaggag agaagaaatg gcagcagtaa
     6781 caggtattgc actgggtatg attgaaacac gtggtctggt tccagcgatt gaagctgccg
     6841 atgccatgac caaggccgcc gaagtacgtt tggttggccg tcaatttgtt ggtggtggtt
     6901 acgtgaccgt tttggtccgt ggtgaaaccg gtgccgtcaa cgcagcagtt cgtgcgggcg
     6961 ctgatgcctg cgaacgagtc ggcgatggtc tggtcgcggc gcatatcatt gcccgtgtcc
     7021 attccgaagt cgaaaacatc ctgccgaaag cccctgaagc ttaaggattg ggaaagacga
     7081 accggcgcag gcttgttccg gttctttgca taaagtgaca gcttaggagt ttatttaaat
     7141 ggctgatgta actggtattg ctctgggtat gatcgaaaca cgtggcttag ttcctgcgat
     7201 tgaagcagcg gacgccatga ctaaagcggc tgaagtgcgt ttggtcggtc gtcaatttgt
     7261 tggtggcggt tacgtcaccg tattggttcg gggcgaaaca ggcgctgtta acgccgctgt
     7321 tcgtgctggc gccgatgctt gcgaacgtgt tggtgatggc ttggttgctg cgcacatcat
     7381 tgcgcgtgtc cactcagaag tagaaaacat tctgcctaag gcgccacaag cctaagtcag
     7441 atattcctaa gacggctcac ttcggcgaca cccgtcgccg aaaccgtcta accaaatttc
     7501 tgggctcgtt ctgaacgtgc tcaacactag ttttagagga tctgttatgg caacgactca
     7561 cggtattgcc ctgggcatga ttgaaacacg aggattggtt cctgccattg aagccgcaga
     7621 tgccatgacc aaagcggcgg aagtccgtct ggtcggacga tcatttgttg gcggcggtta
     7681 cgtgaccgta atggttcgtg gtgagacagg tgcagtaaat gctgccgttc gtgcgggtgc
     7741 tgacgcctgt gaacgtgttg gcgatggcct ggttgctgcg cacatcattg cgcgcgttca
     7801 ttctgaagtt gagatcatcc tacccgagac gcccgaagac tcagattccg cgtggtgtat
     7861 cgcaaatctg aatagctaat gtctagtagg gaagatgcgc atgaacaaca ttgatttgcg
     7921 cgtctattcg tttatcgatt cgcttcaacc gcagcttgca tcctatcttg cgacatcatc
     7981 gcaaggcttt cttcccgttc cgggtgatgc ctgcttgtgg attgaagtcg cgccgggcat
     8041 ggccgtccat cgcctcagtg atattgcgct aaaggccacg aacgttcgtc tcggcgaaca
     8101 ggtagtcgag cgtgcttttg gctcgatgga aattcattac cgaaaccaaa gcgacgttct
     8161 cgcatccggt gaggccgttt taagagaaat caatcacgcg caagaagatc gtctgccttg
     8221 tcgcatcgca tggaaagaga tcattcgagc gattaccccc gatcatgcca ccttgatcaa
     8281 tcgccagttg cgtaaaggct ctatgctgtt gccgggcaaa agcatgttca tccttgaaac
     8341 agaaccggca ggttatattg ttcaggctgc caacgaggcc gagaaagcag ctcatgtgac
     8401 tctgatcgat gtacgtgctt ttggtaactt tggtcgcctg accatgatgg gcagcgaagc
     8461 ggaaaccgaa gaagccatgc gggcggctga ggccacaatc gcaagcatca acgcgcgtgc
     8521 gcgtcgcgct gaagggttct aagcggccgc gaggttccaa ctttcaccat aatgaaataa
     8581 gatcactacc gggcgtattt tttgagttat cgagattttc aggagctaag gaagctaaaa
     8641 tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac
     8701 attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata
     8761 ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc
     8821 acattcttgc ccgcctgatg aatgctcatc cggagttccg tatggcaatg aaagacggtg
     8881 agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa
     8941 cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt
     9001 cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga
     9061 atatgttttt cgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg
     9121 ccaatatgga caacttcttc gcccccgttt tcactatggg caaatattat acgcaaggcg
     9181 acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg
     9241 tcggcagaat gcttaatgaa ttacaacagt actgcgatga gtggcagggc ggggcgtaat
     9301 ttttttaagg cagttattgg tgcccttgaa ttcctactag tcgaagcggc atgcatttac
     9361 gttgacacca tcgaatggtg caaaaccttt cgcggtatgg catgatagcg cccggaagag
     9421 agtcaattca gggtggtgaa tgtgaaacca gtaacgttat acgatgtcgc agagtatgcc
     9481 ggtgtctctt atcagaccgt ttcccgcgtg gtgaaccagg ccagccacgt ttctgcgaaa
     9541 acgcgggaaa aagtggaagc ggcgatggcg gagctgaatt acattcccaa ccgcgtggca
     9601 caacaactgg cgggcaaaca gtcgttgctg attggcgttg ccacctccag tctggccctg
     9661 cacgcgccgt cgcaaattgt cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc
     9721 gtggtggtgt cgatggtaga acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat
     9781 cttctcgcgc aacgcgtcag tgggctgatc attaactatc cgctggatga ccaggatgcc
     9841 attgctgtgg aagctgcctg cactaatgtt ccggcgttat ttcttgatgt ctctgaccag
     9901 acacccatca acagtattat tttctcccat gaagacggta cgcgactggg cgtggagcat
     9961 ctggtcgcat tgggtcacca gcaaatcgcg ctgttagcgg gcccattaag ttctgtctcg
    10021 gcgcgtctgc gtctggctgg ctggcataaa tatctcactc gcaatcaaat tcagccgata
    10081 gcggaacggg aaggcgactg gagtgccatg tccggttttc aacaaaccat gcaaatgctg
    10141 aatgagggca tcgttcccac tgcgatgctg gttgccaacg atcagatggc gctgggcgca
    10201 atgcgcgcca ttaccgagtc cgggctgcgc gttggtgcgg atatctcggt agtgggatac
    10261 gacgataccg aagacagctc atgttatatc ccgccgttaa ccaccatcaa acaggatttt
    10321 cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg
    10381 aagggcaatc agctgttgcc cgtctcactg gtgaaaagaa aaaccaccct ggcgcccaat
    10441 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
    10501 tcccgactgg aaagcgggca gtgatcccac agccgccagt tccgctggcg gcattttaac
    10561 tttctttaat aatctagtga caagccgggg cagacgtgag ccgtagtccc gtcgccagac
    10621 gcgggtgccc acgggcgtcg tcaggatgtc cgtaattgac tgccggaggt ggtcaatgcc
    10681 cttcagctcc ttgccactgt cacggctcat gcctcgggtc attagtcgcc cgctccggta
    10741 tcttcactgg cttcgatgat tgccgccccg cagctgcaga ggtcaccgat ccgagcagtc
    10801 ggcctctggt tggtaaagac cgtgcgactg ccggtgatga tcgtgttcaa gccatgcagg
    10861 gggcaggcgt gaaggtcatc ctttcgggcc acggggcggc tgttgacgaa ggtgtcgtcg
    10921 ctcccggtga tgatgatccc gccgtgatcg gtcacgtcgt ttagtcgagc gatgcctggc
    10981 gtcgtagtca cgggtttagg tcaatacgac ttgcggtcac tgtaacgttg ccctcggcgg
    11041 tcacgttaac gtcgccttgg gcttcgactt gcgcctcctg cacaaggatc acaatccgtc
    11101 cttgggctgc ggtgaggtcg atcttgtact catgcgcttc gcggtcgtac tggatgattg
    11161 agtcatcctc gaactgcgtc ttttggatcg tttctttgtc ctcgatctgg gggtagtcag
    11221 tcgagaacgc gccgggcatc gcgaagccct gactgatctc gccggagggg gccatcacga
    11281 cgacggcctc accgacctcg ggcgcccacc agaaccgatc cttgcccgct cgctgcgtga
    11341 gccacggaat ccagtcagtg aggagcagcg cctcgccgct ctcctcgtct tcctcgatcg
    11401 cgacacggat cagccccttg ggatagtcag cctcggctac cctgcctacg cggagcaagt
    11461 tgccgtgacg ccgactgtct cgagtctagt cgcgttgctg gcgtttttcc ataggctccg
    11521 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
    11581 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
    11641 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
    11701 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
    11761 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
    11821 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
    11881 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
    11941 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
    12001 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
    12061 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
    12121 gtctgacgct cagtctagtt cagccagctc gtcgtgatgt cgaaacccaa gccaccctca
    12181 gaggtgaagg ccgcttcgag cacatcggga agcgtgtcga cgacggtgct cggtgcctcg
    12241 ggtaagagcc agatagatgc ggtgacgttc accgtcgcga tcgtggcggg aaccacggtc
    12301 actgcgtccg ttacaactcg cacgtcgtcg gccagcacga ctgactcgac ggcttcgatt
    12361 agcccgggag aggcaaggcc atcaggctcg gtagacagaa tgctgattaa cacctcgccg
    12421 ggagcagggc tgctcacagc cgcgtccttc acccgtgggt cagccgtcag cgcttgatag
    12481 cggtaccagg ccgcaccgcc tgcggtcgag ctgcccttga tccgctcgat ggtgcgatcg
    12541 cgaagctcgc catccggctc attcggctgc cgcaccacgc cgtagaacgc ggcaaggttg
    12601 tcgaggtcgg ggccgtttga gtagcgaagc agtgtcgcaa gaagtgcgtc gttgatccgc
    12661 tgacgcagga tcagctcgcg ggctgcgcag acctccagca gcttgatgac cgggtcggat
    12721 tcgaggatgg cggtgtagct ggcgtcacgc gatcgcaggt cgtcgatcaa gtcctgcagg
    12781 atcagttcaa agtccagcgc ttcgatgatg gtgggcgcgg gaatcgtagc aaagtcaaga
    12841 acggtcatga gacgactaag ccctccagcg tgatacgcct gccctcgggg atgtag
//
