LOCUS       sequence-300516-       10989 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Modified Tobacco rattle virus RNA2 vector that could be used for 
            expression of sgRNAs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10989)
  AUTHORS   Ellison EE, Nagalakshmi U, Gamo ME, Huang PJ, Dinesh-Kumar S, Voytas
            DF
  TITLE     Multiplexed heritable gene editing using RNA viruses and mobile 
            single guide RNAs.
  JOURNAL   Nat Plants. 2020 Jun 1. pii: 10.1038/s41477-020-0670-y. doi: 
            10.1038/s41477-020-0670-y.
  PUBMED    32483329
REFERENCE   2  (bases 1 to 10989)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Plants.
            2020 Jun 1. pii: 10.1038/s41477-020-0670-y. doi: 
            10.1038/s41477-020-0670-y."
COMMENT     Sequence Label: SPDK3889(TRV-RNA2-pPEBV-MCS-AtMet-tRNA)
FEATURES             Location/Qualifiers
     source          1..10989
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        427..772
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     715..735
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             2919..2936
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      2971..3223
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    3245..3269
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4570..5199
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5628..6701
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      6767..6961
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(7197..7219)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7305..7445
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7360..7379
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7460..7477)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7631..8219)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7711..7730)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9644..10438)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    10863..10887
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 aagcttgcat gcctgcaggt caacatggtg gagcacgaca ctctcgtcta ctccaagaat
       61 atcaaagata cagtctcaga agaccagagg gctattgaga cttttcaaca aagggtaata
      121 tcgggaaacc tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta
      181 gaaaaggaag atggcttcta caaatgccat cattgcgata aaggaaaggc tatcgttcaa
      241 gatgcctcta ccgacagtgg tcccaaagat ggacccccac ccacgaggaa catcgtggaa
      301 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgatgg tcaacatggt
      361 ggagcacgac actctcgtct actccaagaa tatcaaagat acagtctcag aagaccagag
      421 ggctattgag acttttcaac aaagggtaat atcgggaaac ctcctcggat tccattgccc
      481 agctatctgt cacttcatcg aaaggacagt agaaaaggaa gatggcttct acaaatgcca
      541 tcattgcgat aaaggaaagg ctatcgttca agatgcctct accgacagtg gtcccaaaga
      601 tggaccccca cccacgagga acatcgtgga aaaagaagac gttccaacca cgtcttcaaa
      661 gcaagtggat tgatgtgata tctccactga cgtaagggat gacgcacaat cccactatcc
      721 ttcgcaagac ccttcctcta tataaggaag ttcatttcat ttggagagga taaaacattg
      781 cacctatggt gttgccctgg ctggggtatg tcagtgatcg cagtagaatg tactaattga
      841 caagttggag aatacggtag aacgtcctta tccaacacag cctttatccc tctccctgac
      901 gaggtttttg tcagtgtaat atttcttttt gaactatcca gcttagtacc gtacgggaaa
      961 gtgactggtg tgcttatctt tgaaatgtta ctttgggttt cggttcttta ggttagtaag
     1021 aaagcacttg tcttctcata caaaggaaaa cctgagacgt atcgcttacg aaagtagcaa
     1081 tgaaagaaag gtggtggttt taatcgctac cgcaaaaacg atggggtcgt tttaattaac
     1141 ttctcctacg caagcgtcta aacggacgtt ggggttttgc tagtttcttt agagaaaact
     1201 agctaagtct ttaatgttat cattagagat ggcataaata taatacttgt gtctgctgat
     1261 aagatcattt taatttggac gattagactt gttgaactac aggttactga atcacttgcg
     1321 ctaatcaaca tgggagatat gtacgatgaa tcatttgaca agtcgggcgg tcctgctgac
     1381 ttgatggacg attcttgggt ggaatcagtt tcgtggaaag atctgttgaa gaagttacac
     1441 agcataaaat ttgcactaca gtctggtaga gatgagatca ctgggttact agcggcactg
     1501 aatagacagt gtccttattc accatatgag cagtttccag ataagaaggt gtatttcctt
     1561 ttagactcac gggctaacag tgctcttggt gtgattcaga acgcttcagc gttcaagaga
     1621 cgagctgatg agaagaatgc agtggcgggt gttacaaata ttcctgcgaa tccaaacaca
     1681 acggttacga cgaaccaagg gagtactact actaccaagg cgaacactgg ctcgactttg
     1741 gaagaagact tgtacactta ttacaaattc gatgatgcct ctacagcttt ccacaaatct
     1801 ctaacttcgt tagagaacat ggagttgaag agttattacc gaaggaactt tgagaaagta
     1861 ttcgggatta agtttggtgg agcagctgct agttcatctg caccgcctcc agcgagtgga
     1921 ggtccgatac gtcctaatcc ctagggattt aaggacgtga actctgttga gatctctgtg
     1981 aaattcagag ggtgggtgat accatattca ctgatgccat tagcgacatc taaatagggc
     2041 taattgtgac taatttgagg gaatttcctt taccattgac gtcagtgtcg ttggtagcat
     2101 ttgagtttcg gcacacaagg ttaaaaacgc tgtagtaata catgcgcaag aacaggctga
     2161 gcatcttgtt ctggggtttc acactatctt tagagaaagt gttaagttaa ttaagttatc
     2221 ttaattaaga gcataattat actgatttgt ctctcgttga tagagtctat cattctgtta
     2281 ttaaaaattt gacaactcgg tttgctgacc tactggtcac tgtatcactt acccgagtta
     2341 acgagtctag aaggcctcca tggggatccg gtaccgagct ctgaatcaga gtggcgcagc
     2401 ggaagcgtgg tgggcccata acccacaggt cccaggatcg aaacctggct ctgatacccg
     2461 ggcatgtccc gaagacatta aactacggtt ctttaagtag atccgtgtct gaagttttag
     2521 gttcaattta aacctacgag attgacattc tcgactgatc ttgattgatc ggtaagtctt
     2581 ttgtaattta attttctttt tgattttatt ttaaattgtt atctgtttct gtgtatagac
     2641 tgtttgagat cggcgtttgg ccgactcatt gtcttaccat aggggaacgg actttgtttg
     2701 tgttgttatt ttatttgtat tttattaaaa ttctcaacga tctgaaaaag cctcgcggct
     2761 aagagattgt tggggggtga gtaagtactt ttaaagtgat gatggttaca aaggcaaaag
     2821 gggtaaaacc cctcgcctac gtaagcgtta ttacgcccgt ctgtacttat atcagtacac
     2881 tgacgagtcc ctaaaggacg aaacgggaga acgctagcca ccaccaccac caccacgtgt
     2941 gaattacagg tgaccagctc gaatttcccc gatcgttcaa acatttggca ataaagtttc
     3001 ttaagattga atcctgttgc cggtcttgcg atgattatca tataatttct gttgaattac
     3061 gttaagcatg taataattaa catgtaatgc atgacgttat ttatgagatg ggtttttatg
     3121 attagagtcc cgcaattata catttaatac gcgatagaaa acaaaatata gcgcgcaaac
     3181 taggataaat tatcgcgcgc ggtgtcatct atgttactag atcgggaatt aaactatcag
     3241 tgtttgacag gatatattgg cgggtaaacc taagagaaaa gagcgtttat tagaataatc
     3301 ggatatttaa aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt gcatgccaac
     3361 cacagggttc ccctcgggat caaagtactt tgatccaacc cctccgctgc tatagtgcag
     3421 tcggcttctg acgttcagtg cagccgtctt ctgaaaacga catgtcgcac aagtcctaag
     3481 ttacgcgaca ggctgccgcc ctgccctttt cctggcgttt tcttgtcgcg tgttttagtc
     3541 gcataaagta gaatacttgc gactagaacc ggagacatta cgccatgaac aagagcgccg
     3601 ccgctggcct gctgggctat gcccgcgtca gcaccgacga ccaggacttg accaaccaac
     3661 gggccgaact gcacgcggcc ggctgcacca agctgttttc cgagaagatc accggcacca
     3721 ggcgcgaccg cccggagctg gccaggatgc ttgaccacct acgccctggc gacgttgtga
     3781 cagtgaccag gctagaccgc ctggcccgca gcacccgcga cctactggac attgccgagc
     3841 gcatccagga ggccggcgcg ggcctgcgta gcctggcaga gccgtgggcc gacaccacca
     3901 cgccggccgg ccgcatggtg ttgaccgtgt tcgccggcat tgccgagttc gagcgttccc
     3961 taatcatcga ccgcacccgg agcgggcgcg aggccgccaa ggcccgaggc gtgaagtttg
     4021 gcccccgccc taccctcacc ccggcacaga tcgcgcacgc ccgcgagctg atcgaccagg
     4081 aaggccgcac cgtgaaagag gcggctgcac tgcttggcgt gcatcgctcg accctgtacc
     4141 gcgcacttga gcgcagcgag gaagtgacgc ccaccgaggc caggcggcgc ggtgccttcc
     4201 gtgaggacgc attgaccgag gccgacgccc tggcggccgc cgagaatgaa cgccaagagg
     4261 aacaagcatg aaaccgcacc aggacggcca ggacgaaccg tttttcatta ccgaagagat
     4321 cgaggcggag atgatcgcgg ccgggtacgt gttcgagccg cccgcgcacg tctcaaccgt
     4381 gcggctgcat gaaatcctgg ccggtttgtc tgatgccaag ctggcggcct ggccggccag
     4441 cttggccgct gaagaaaccg agcgccgccg tctaaaaagg tgatgtgtat ttgagtaaaa
     4501 cagcttgcgt catgcggtcg ctgcgtatat gatgcgatga gtaaataaac aaatacgcaa
     4561 ggggaacgca tgaaggttat cgctgtactt aaccagaaag gcgggtcagg caagacgacc
     4621 atcgcaaccc atctagcccg cgccctgcaa ctcgccgggg ccgatgttct gttagtcgat
     4681 tccgatcccc agggcagtgc ccgcgattgg gcggccgtgc gggaagatca accgctaacc
     4741 gttgtcggca tcgaccgccc gacgattgac cgcgacgtga aggccatcgg ccggcgcgac
     4801 ttcgtagtga tcgacggagc gccccaggcg gcggacttgg ctgtgtccgc gatcaaggca
     4861 gccgacttcg tgctgattcc ggtgcagcca agcccttacg acatatgggc caccgccgac
     4921 ctggtggagc tggttaagca gcgcattgag gtcacggatg gaaggctaca agcggccttt
     4981 gtcgtgtcgc gggcgatcaa aggcacgcgc atcggcggtg aggttgccga ggcgctggcc
     5041 gggtacgagc tgcccattct tgagtcccgt atcacgcagc gcgtgagcta cccaggcact
     5101 gccgccgccg gcacaaccgt tcttgaatca gaacccgagg gcgacgctgc ccgcgaggtc
     5161 caggcgctgg ccgctgaaat taaatcaaaa ctcatttgag ttaatgaggt aaagagaaaa
     5221 tgagcaaaag cacaaacacg ctaagtgccg gccgtccgag cgcacgcagc agcaaggctg
     5281 caacgttggc cagcctggca gacacgccag ccatgaagcg ggtcaacttt cagttgccgg
     5341 cggaggatca caccaagctg aagatgtacg cggtacgcca aggcaagacc attaccgagc
     5401 tgctatctga atacatcgcg cagctaccag agtaaatgag caaatgaata aatgagtaga
     5461 tgaattttag cggctaaagg aggcggcatg gaaaatcaag aacaaccagg caccgacgcc
     5521 gtggaatgcc ccatgtgtgg aggaacgggc ggttggccag gcgtaagcgg ctgggttgcc
     5581 tgccggccct gcaatggcac tggaaccccc aagcccgagg aatcggcgtg agcggtcgca
     5641 aaccatccgg cccggtacaa atcggcgcgg cgctgggtga tgacctggtg gagaagttga
     5701 aggccgcgca ggccgcccag cggcaacgca tcgaggcaga agcacgcccc ggtgaatcgt
     5761 ggcaagcggc cgctgatcga atccgcaaag aatcccggca accgccggca gccggtgcgc
     5821 cgtcgattag gaagccgccc aagggcgacg agcaaccaga ttttttcgtt ccgatgctct
     5881 atgacgtggg cacccgcgat agtcgcagca tcatggacgt ggccgttttc cgtctgtcga
     5941 agcgtgaccg acgagctggc gaggtgatcc gctacgagct tccagacggg cacgtagagg
     6001 tttccgcagg gccggccggc atggccagtg tgtgggatta cgacctggta ctgatggcgg
     6061 tttcccatct aaccgaatcc atgaaccgat accgggaagg gaagggagac aagcccggcc
     6121 gcgtgttccg tccacacgtt gcggacgtac tcaagttctg ccggcgagcc gatggcggaa
     6181 agcagaaaga cgacctggta gaaacctgca ttcggttaaa caccacgcac gttgccatgc
     6241 agcgtacgaa gaaggccaag aacggccgcc tggtgacggt atccgagggt gaagccttga
     6301 ttagccgcta caagatcgta aagagcgaaa ccgggcggcc ggagtacatc gagatcgagc
     6361 tagctgattg gatgtaccgc gagatcacag aaggcaagaa cccggacgtg ctgacggttc
     6421 accccgatta ctttttgatc gatcccggca tcggccgttt tctctaccgc ctggcacgcc
     6481 gcgccgcagg caaggcagaa gccagatggt tgttcaagac gatctacgaa cgcagtggca
     6541 gcgccggaga gttcaagaag ttctgtttca ccgtgcgcaa gctgatcggg tcaaatgacc
     6601 tgccggagta cgatttgaag gaggaggcgg ggcaggctgg cccgatccta gtcatgcgct
     6661 accgcaacct gatcgagggc gaagcatccg ccggttccta atgtacggag cagatgctag
     6721 ggcaaattgc cctagcaggg gaaaaaggtc gaaaaggtct ctttcctgtg gatagcacgt
     6781 acattgggaa cccaaagccg tacattggga accggaaccc gtacattggg aacccaaagc
     6841 cgtacattgg gaaccggtca cacatgtaag tgactgatat aaaagagaaa aaaggcgatt
     6901 tttccgccta aaactcttta aaacttatta aaactcttaa aacccgcctg gcctgtgcat
     6961 aactgtctgg ccagcgcaca gccgaagagc tgcaaaaagc gcctaccctt cggtcgctgc
     7021 gctccctacg ccccgccgct tcgcgtcggc ctatcgcggc cgctggccgc tcaaaaatgg
     7081 ctggcctacg gccaggcaat ctaccagggc gcggacaagc cgcgccgtcg ccactcgacc
     7141 gccggcgccc acatcaaggc accctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc
     7201 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
     7261 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag
     7321 tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag cagattgtac
     7381 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
     7441 tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     7501 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     7561 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     7621 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     7681 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     7741 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     7801 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     7861 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     7921 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     7981 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     8041 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
     8101 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     8161 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     8221 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     8281 ggctgatgaa tcccctaatg atttttatca aaatcattaa gttaaggtag atacacatct
     8341 tgtcatatga tcaaatggtt tcgccaaaaa tcaataatca gacaacaaaa tgtgcgaact
     8401 cgatatttta cacgactctc tttaccaatt ctgccccgaa ttacacttaa aacgactcaa
     8461 cagcttaacg ttggcttgcc acgccttact tgactgtaaa actctcactc ttaccgaact
     8521 tggccgtaac ctgccaacca aagcgagaac aaaacataac atcaaacgaa tcgaccgatt
     8581 gttaggtaat cgtcacctcc acaaagagcg actcgctgta taccgttggc atgctagctt
     8641 tatctgttcg ggcaatacga tgcccattgt acttgttgac tggtctgata tccgtgagca
     8701 aaaacggctt atggtattgc gagcttcagt cgcactacac ggtcgttctg ttactcttta
     8761 tgagaaagcg ttcccgcttt cagagcaatg ttcaaagaaa gctcatgacc aatttctagc
     8821 cgaccttgcg agcattctac cgagtaacac cacaccgctc attgtcagtg atgctggctt
     8881 taaagtgcca tggtataaat ccgttgagaa gctgggttgg tactggttaa gtcgagtaag
     8941 aggaaaagta caatatgcag acctaggagc ggaaaactgg aaacctatca gcaacttaca
     9001 tgatatgtca tctagtcact caaagacttt aggctataag aggctgacta aaagcaatcc
     9061 aatctcatgc caaattctat tgtataaatc tcgctctaaa ggccgaaaaa atcagcgctc
     9121 gacacggact cattgtcacc acccgtcacc taaaatctac tcagcgtcgg caaaggagcc
     9181 atggattcta gcaactaact tacctgttga aattcgaaca cccaaacaac ttgttaatat
     9241 ctattcgaag cgaatgcaga ttgaagaaac cttccgagac ttgaaaagtc ctgcctacgg
     9301 actaggccta cgccatagcc gaacgagcag ctcagagcgt tttgatatca tgctgctaat
     9361 cgccctgatg cttcaactaa catgttggct tgcgggcgtt catgctcaga aacaaggttg
     9421 ggacaagcac ttccaggcta acacagtcag aaatcgaaac gtactctcaa cagttcgctt
     9481 aggcatggaa gttttgcggc attctggcta cacaataaca agggaagact cactcgtggc
     9541 tgcaaccctg cttactcaaa atctattcac acatggttac gttttgggga aattatgagg
     9601 ggatctctca gcgttaaggg attttggtca tgcattctag gtactaaaac aattcatcca
     9661 gtaaaatata atattttatt ttctcccaat caggcttgat ccccagtaag tcaaaaaata
     9721 gctcgacata ctgttcttcc ccgatatcct ccctgatcga ccggacgcag aaggcaatgt
     9781 cataccactt gtccgccctg ccgcttctcc caagatcaat aaagccactt actttgccat
     9841 ctttcacaaa gatgttgctg tctcccaggt cgccgtggga aaagacaagt tcctcttcgg
     9901 gcttttccgt ctttaaaaaa tcatacagct cgcgcggatc tttaaatgga gtgtcttctt
     9961 cccagttttc gcaatccaca tcggccagat cgttattcag taagtaatcc aattcggcta
    10021 agcggctgtc taagctattc gtatagggac aatccgatat gtcgatggag tgaaagagcc
    10081 tgatgcactc cgcatacagc tcgataatct tttcagggct ttgttcatct tcatactctt
    10141 ccgagcaaag gacgccatcg gcctcactca tgagcagatt gctccagcca tcatgccgtt
    10201 caaagtgcag gacctttgga acaggcagct ttccttccag ccatagcatc atgtcctttt
    10261 cccgttccac atcataggtg gtccctttat accggctgtc cgtcattttt aaatataggt
    10321 tttcattttc tcccaccagc ttatatacct tagcaggaga cattccttcc gtatctttta
    10381 cgcagcggta tttttcgatc agttttttca attccggtga tattctcatt ttagccattt
    10441 attatttcct tcctcttttc tacagtattt aaagataccc caagaagcta attataacaa
    10501 gacgaactcc aattcactgt tccttgcatt ctaaaacctt aaataccaga aaacagcttt
    10561 ttcaaagttg ttttcaaagt tggcgtataa catagtatcg acggagccga ttttgaaacc
    10621 gcggtgatca caggcagcaa cgctctgtca tcgttacaat caacatgcta ccctccgcga
    10681 gatcatccgt gtttcaaacc cggcagctta gttgccgttc ttccgaatag catcggtaac
    10741 atgagcaaag tctgccgcct tacaacggct ctcccgctga cgccgtcccg gactgatggg
    10801 ctgcctgtat cgagtggtga ttttgtgccg agctgccggt cggggagctg ttggctggct
    10861 ggtggcagga tatattgtgg tgtaaacaaa ttgacgctta gacaacttaa taacacattg
    10921 cggacgtttt taatgtactg aattaacgcc gaattaattc ctaggccacc atgttgggcc
    10981 cggcgcgcc
//
