LOCUS       sequence-300518-       10988 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Modified Tobacco rattle virus RNA2 vector that could be used for 
            expression of sgRNAs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10988)
  AUTHORS   Ellison EE, Nagalakshmi U, Gamo ME, Huang PJ, Dinesh-Kumar S, Voytas
            DF
  TITLE     Multiplexed heritable gene editing using RNA viruses and mobile 
            single guide RNAs.
  JOURNAL   Nat Plants. 2020 Jun 1. pii: 10.1038/s41477-020-0670-y. doi: 
            10.1038/s41477-020-0670-y.
  PUBMED    32483329
REFERENCE   2  (bases 1 to 10988)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Plants.
            2020 Jun 1. pii: 10.1038/s41477-020-0670-y. doi: 
            10.1038/s41477-020-0670-y."
COMMENT     Sequence Label: SPDK3902(TRV-RNA2-pPEBV-MCS-AtGly-tRNA)
FEATURES             Location/Qualifiers
     source          1..10988
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        427..772
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     715..735
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             2918..2935
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      2970..3222
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    3244..3268
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4569..5198
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5627..6700
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      6766..6960
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(7196..7218)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7304..7444
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7359..7378
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7459..7476)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7630..8218)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7710..7729)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9643..10437)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    10862..10886
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 aagcttgcat gcctgcaggt caacatggtg gagcacgaca ctctcgtcta ctccaagaat
       61 atcaaagata cagtctcaga agaccagagg gctattgaga cttttcaaca aagggtaata
      121 tcgggaaacc tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta
      181 gaaaaggaag atggcttcta caaatgccat cattgcgata aaggaaaggc tatcgttcaa
      241 gatgcctcta ccgacagtgg tcccaaagat ggacccccac ccacgaggaa catcgtggaa
      301 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgatgg tcaacatggt
      361 ggagcacgac actctcgtct actccaagaa tatcaaagat acagtctcag aagaccagag
      421 ggctattgag acttttcaac aaagggtaat atcgggaaac ctcctcggat tccattgccc
      481 agctatctgt cacttcatcg aaaggacagt agaaaaggaa gatggcttct acaaatgcca
      541 tcattgcgat aaaggaaagg ctatcgttca agatgcctct accgacagtg gtcccaaaga
      601 tggaccccca cccacgagga acatcgtgga aaaagaagac gttccaacca cgtcttcaaa
      661 gcaagtggat tgatgtgata tctccactga cgtaagggat gacgcacaat cccactatcc
      721 ttcgcaagac ccttcctcta tataaggaag ttcatttcat ttggagagga taaaacattg
      781 cacctatggt gttgccctgg ctggggtatg tcagtgatcg cagtagaatg tactaattga
      841 caagttggag aatacggtag aacgtcctta tccaacacag cctttatccc tctccctgac
      901 gaggtttttg tcagtgtaat atttcttttt gaactatcca gcttagtacc gtacgggaaa
      961 gtgactggtg tgcttatctt tgaaatgtta ctttgggttt cggttcttta ggttagtaag
     1021 aaagcacttg tcttctcata caaaggaaaa cctgagacgt atcgcttacg aaagtagcaa
     1081 tgaaagaaag gtggtggttt taatcgctac cgcaaaaacg atggggtcgt tttaattaac
     1141 ttctcctacg caagcgtcta aacggacgtt ggggttttgc tagtttcttt agagaaaact
     1201 agctaagtct ttaatgttat cattagagat ggcataaata taatacttgt gtctgctgat
     1261 aagatcattt taatttggac gattagactt gttgaactac aggttactga atcacttgcg
     1321 ctaatcaaca tgggagatat gtacgatgaa tcatttgaca agtcgggcgg tcctgctgac
     1381 ttgatggacg attcttgggt ggaatcagtt tcgtggaaag atctgttgaa gaagttacac
     1441 agcataaaat ttgcactaca gtctggtaga gatgagatca ctgggttact agcggcactg
     1501 aatagacagt gtccttattc accatatgag cagtttccag ataagaaggt gtatttcctt
     1561 ttagactcac gggctaacag tgctcttggt gtgattcaga acgcttcagc gttcaagaga
     1621 cgagctgatg agaagaatgc agtggcgggt gttacaaata ttcctgcgaa tccaaacaca
     1681 acggttacga cgaaccaagg gagtactact actaccaagg cgaacactgg ctcgactttg
     1741 gaagaagact tgtacactta ttacaaattc gatgatgcct ctacagcttt ccacaaatct
     1801 ctaacttcgt tagagaacat ggagttgaag agttattacc gaaggaactt tgagaaagta
     1861 ttcgggatta agtttggtgg agcagctgct agttcatctg caccgcctcc agcgagtgga
     1921 ggtccgatac gtcctaatcc ctagggattt aaggacgtga actctgttga gatctctgtg
     1981 aaattcagag ggtgggtgat accatattca ctgatgccat tagcgacatc taaatagggc
     2041 taattgtgac taatttgagg gaatttcctt taccattgac gtcagtgtcg ttggtagcat
     2101 ttgagtttcg gcacacaagg ttaaaaacgc tgtagtaata catgcgcaag aacaggctga
     2161 gcatcttgtt ctggggtttc acactatctt tagagaaagt gttaagttaa ttaagttatc
     2221 ttaattaaga gcataattat actgatttgt ctctcgttga tagagtctat cattctgtta
     2281 ttaaaaattt gacaactcgg tttgctgacc tactggtcac tgtatcactt acccgagtta
     2341 acgagtctag aaggcctcca tggggatccg gtaccgagct ctgagcacca gtggtctagt
     2401 ggcatgatag taccctgcca cggtacagac ccgggttcaa ttcccggctg gtgcacccgg
     2461 gcatgtcccg aagacattaa actacggttc tttaagtaga tccgtgtctg aagttttagg
     2521 ttcaatttaa acctacgaga ttgacattct cgactgatct tgattgatcg gtaagtcttt
     2581 tgtaatttaa ttttcttttt gattttattt taaattgtta tctgtttctg tgtatagact
     2641 gtttgagatc ggcgtttggc cgactcattg tcttaccata ggggaacgga ctttgtttgt
     2701 gttgttattt tatttgtatt ttattaaaat tctcaacgat ctgaaaaagc ctcgcggcta
     2761 agagattgtt ggggggtgag taagtacttt taaagtgatg atggttacaa aggcaaaagg
     2821 ggtaaaaccc ctcgcctacg taagcgttat tacgcccgtc tgtacttata tcagtacact
     2881 gacgagtccc taaaggacga aacgggagaa cgctagccac caccaccacc accacgtgtg
     2941 aattacaggt gaccagctcg aatttccccg atcgttcaaa catttggcaa taaagtttct
     3001 taagattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg ttgaattacg
     3061 ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg gtttttatga
     3121 ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag cgcgcaaact
     3181 aggataaatt atcgcgcgcg gtgtcatcta tgttactaga tcgggaatta aactatcagt
     3241 gtttgacagg atatattggc gggtaaacct aagagaaaag agcgtttatt agaataatcg
     3301 gatatttaaa agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg catgccaacc
     3361 acagggttcc cctcgggatc aaagtacttt gatccaaccc ctccgctgct atagtgcagt
     3421 cggcttctga cgttcagtgc agccgtcttc tgaaaacgac atgtcgcaca agtcctaagt
     3481 tacgcgacag gctgccgccc tgcccttttc ctggcgtttt cttgtcgcgt gttttagtcg
     3541 cataaagtag aatacttgcg actagaaccg gagacattac gccatgaaca agagcgccgc
     3601 cgctggcctg ctgggctatg cccgcgtcag caccgacgac caggacttga ccaaccaacg
     3661 ggccgaactg cacgcggccg gctgcaccaa gctgttttcc gagaagatca ccggcaccag
     3721 gcgcgaccgc ccggagctgg ccaggatgct tgaccaccta cgccctggcg acgttgtgac
     3781 agtgaccagg ctagaccgcc tggcccgcag cacccgcgac ctactggaca ttgccgagcg
     3841 catccaggag gccggcgcgg gcctgcgtag cctggcagag ccgtgggccg acaccaccac
     3901 gccggccggc cgcatggtgt tgaccgtgtt cgccggcatt gccgagttcg agcgttccct
     3961 aatcatcgac cgcacccgga gcgggcgcga ggccgccaag gcccgaggcg tgaagtttgg
     4021 cccccgccct accctcaccc cggcacagat cgcgcacgcc cgcgagctga tcgaccagga
     4081 aggccgcacc gtgaaagagg cggctgcact gcttggcgtg catcgctcga ccctgtaccg
     4141 cgcacttgag cgcagcgagg aagtgacgcc caccgaggcc aggcggcgcg gtgccttccg
     4201 tgaggacgca ttgaccgagg ccgacgccct ggcggccgcc gagaatgaac gccaagagga
     4261 acaagcatga aaccgcacca ggacggccag gacgaaccgt ttttcattac cgaagagatc
     4321 gaggcggaga tgatcgcggc cgggtacgtg ttcgagccgc ccgcgcacgt ctcaaccgtg
     4381 cggctgcatg aaatcctggc cggtttgtct gatgccaagc tggcggcctg gccggccagc
     4441 ttggccgctg aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac
     4501 agcttgcgtc atgcggtcgc tgcgtatatg atgcgatgag taaataaaca aatacgcaag
     4561 gggaacgcat gaaggttatc gctgtactta accagaaagg cgggtcaggc aagacgacca
     4621 tcgcaaccca tctagcccgc gccctgcaac tcgccggggc cgatgttctg ttagtcgatt
     4681 ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg
     4741 ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc cggcgcgact
     4801 tcgtagtgat cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag
     4861 ccgacttcgt gctgattccg gtgcagccaa gcccttacga catatgggcc accgccgacc
     4921 tggtggagct ggttaagcag cgcattgagg tcacggatgg aaggctacaa gcggcctttg
     4981 tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag gcgctggccg
     5041 ggtacgagct gcccattctt gagtcccgta tcacgcagcg cgtgagctac ccaggcactg
     5101 ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc
     5161 aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt taatgaggta aagagaaaat
     5221 gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc
     5281 aacgttggcc agcctggcag acacgccagc catgaagcgg gtcaactttc agttgccggc
     5341 ggaggatcac accaagctga agatgtacgc ggtacgccaa ggcaagacca ttaccgagct
     5401 gctatctgaa tacatcgcgc agctaccaga gtaaatgagc aaatgaataa atgagtagat
     5461 gaattttagc ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc accgacgccg
     5521 tggaatgccc catgtgtgga ggaacgggcg gttggccagg cgtaagcggc tgggttgcct
     5581 gccggccctg caatggcact ggaaccccca agcccgagga atcggcgtga gcggtcgcaa
     5641 accatccggc ccggtacaaa tcggcgcggc gctgggtgat gacctggtgg agaagttgaa
     5701 ggccgcgcag gccgcccagc ggcaacgcat cgaggcagaa gcacgccccg gtgaatcgtg
     5761 gcaagcggcc gctgatcgaa tccgcaaaga atcccggcaa ccgccggcag ccggtgcgcc
     5821 gtcgattagg aagccgccca agggcgacga gcaaccagat tttttcgttc cgatgctcta
     5881 tgacgtgggc acccgcgata gtcgcagcat catggacgtg gccgttttcc gtctgtcgaa
     5941 gcgtgaccga cgagctggcg aggtgatccg ctacgagctt ccagacgggc acgtagaggt
     6001 ttccgcaggg ccggccggca tggccagtgt gtgggattac gacctggtac tgatggcggt
     6061 ttcccatcta accgaatcca tgaaccgata ccgggaaggg aagggagaca agcccggccg
     6121 cgtgttccgt ccacacgttg cggacgtact caagttctgc cggcgagccg atggcggaaa
     6181 gcagaaagac gacctggtag aaacctgcat tcggttaaac accacgcacg ttgccatgca
     6241 gcgtacgaag aaggccaaga acggccgcct ggtgacggta tccgagggtg aagccttgat
     6301 tagccgctac aagatcgtaa agagcgaaac cgggcggccg gagtacatcg agatcgagct
     6361 agctgattgg atgtaccgcg agatcacaga aggcaagaac ccggacgtgc tgacggttca
     6421 ccccgattac tttttgatcg atcccggcat cggccgtttt ctctaccgcc tggcacgccg
     6481 cgccgcaggc aaggcagaag ccagatggtt gttcaagacg atctacgaac gcagtggcag
     6541 cgccggagag ttcaagaagt tctgtttcac cgtgcgcaag ctgatcgggt caaatgacct
     6601 gccggagtac gatttgaagg aggaggcggg gcaggctggc ccgatcctag tcatgcgcta
     6661 ccgcaacctg atcgagggcg aagcatccgc cggttcctaa tgtacggagc agatgctagg
     6721 gcaaattgcc ctagcagggg aaaaaggtcg aaaaggtctc tttcctgtgg atagcacgta
     6781 cattgggaac ccaaagccgt acattgggaa ccggaacccg tacattggga acccaaagcc
     6841 gtacattggg aaccggtcac acatgtaagt gactgatata aaagagaaaa aaggcgattt
     6901 ttccgcctaa aactctttaa aacttattaa aactcttaaa acccgcctgg cctgtgcata
     6961 actgtctggc cagcgcacag ccgaagagct gcaaaaagcg cctacccttc ggtcgctgcg
     7021 ctccctacgc cccgccgctt cgcgtcggcc tatcgcggcc gctggccgct caaaaatggc
     7081 tggcctacgg ccaggcaatc taccagggcg cggacaagcc gcgccgtcgc cactcgaccg
     7141 ccggcgccca catcaaggca ccctgcctcg cgcgtttcgg tgatgacggt gaaaacctct
     7201 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
     7261 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt
     7321 cacgtagcga tagcggagtg tatactggct taactatgcg gcatcagagc agattgtact
     7381 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat
     7441 caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     7501 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     7561 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     7621 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     7681 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     7741 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     7801 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     7861 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     7921 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     7981 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     8041 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
     8101 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     8161 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     8221 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     8281 gctgatgaat cccctaatga tttttatcaa aatcattaag ttaaggtaga tacacatctt
     8341 gtcatatgat caaatggttt cgccaaaaat caataatcag acaacaaaat gtgcgaactc
     8401 gatattttac acgactctct ttaccaattc tgccccgaat tacacttaaa acgactcaac
     8461 agcttaacgt tggcttgcca cgccttactt gactgtaaaa ctctcactct taccgaactt
     8521 ggccgtaacc tgccaaccaa agcgagaaca aaacataaca tcaaacgaat cgaccgattg
     8581 ttaggtaatc gtcacctcca caaagagcga ctcgctgtat accgttggca tgctagcttt
     8641 atctgttcgg gcaatacgat gcccattgta cttgttgact ggtctgatat ccgtgagcaa
     8701 aaacggctta tggtattgcg agcttcagtc gcactacacg gtcgttctgt tactctttat
     8761 gagaaagcgt tcccgctttc agagcaatgt tcaaagaaag ctcatgacca atttctagcc
     8821 gaccttgcga gcattctacc gagtaacacc acaccgctca ttgtcagtga tgctggcttt
     8881 aaagtgccat ggtataaatc cgttgagaag ctgggttggt actggttaag tcgagtaaga
     8941 ggaaaagtac aatatgcaga cctaggagcg gaaaactgga aacctatcag caacttacat
     9001 gatatgtcat ctagtcactc aaagacttta ggctataaga ggctgactaa aagcaatcca
     9061 atctcatgcc aaattctatt gtataaatct cgctctaaag gccgaaaaaa tcagcgctcg
     9121 acacggactc attgtcacca cccgtcacct aaaatctact cagcgtcggc aaaggagcca
     9181 tggattctag caactaactt acctgttgaa attcgaacac ccaaacaact tgttaatatc
     9241 tattcgaagc gaatgcagat tgaagaaacc ttccgagact tgaaaagtcc tgcctacgga
     9301 ctaggcctac gccatagccg aacgagcagc tcagagcgtt ttgatatcat gctgctaatc
     9361 gccctgatgc ttcaactaac atgttggctt gcgggcgttc atgctcagaa acaaggttgg
     9421 gacaagcact tccaggctaa cacagtcaga aatcgaaacg tactctcaac agttcgctta
     9481 ggcatggaag ttttgcggca ttctggctac acaataacaa gggaagactc actcgtggct
     9541 gcaaccctgc ttactcaaaa tctattcaca catggttacg ttttggggaa attatgaggg
     9601 gatctctcag cgttaaggga ttttggtcat gcattctagg tactaaaaca attcatccag
     9661 taaaatataa tattttattt tctcccaatc aggcttgatc cccagtaagt caaaaaatag
     9721 ctcgacatac tgttcttccc cgatatcctc cctgatcgac cggacgcaga aggcaatgtc
     9781 ataccacttg tccgccctgc cgcttctccc aagatcaata aagccactta ctttgccatc
     9841 tttcacaaag atgttgctgt ctcccaggtc gccgtgggaa aagacaagtt cctcttcggg
     9901 cttttccgtc tttaaaaaat catacagctc gcgcggatct ttaaatggag tgtcttcttc
     9961 ccagttttcg caatccacat cggccagatc gttattcagt aagtaatcca attcggctaa
    10021 gcggctgtct aagctattcg tatagggaca atccgatatg tcgatggagt gaaagagcct
    10081 gatgcactcc gcatacagct cgataatctt ttcagggctt tgttcatctt catactcttc
    10141 cgagcaaagg acgccatcgg cctcactcat gagcagattg ctccagccat catgccgttc
    10201 aaagtgcagg acctttggaa caggcagctt tccttccagc catagcatca tgtccttttc
    10261 ccgttccaca tcataggtgg tccctttata ccggctgtcc gtcattttta aatataggtt
    10321 ttcattttct cccaccagct tatatacctt agcaggagac attccttccg tatcttttac
    10381 gcagcggtat ttttcgatca gttttttcaa ttccggtgat attctcattt tagccattta
    10441 ttatttcctt cctcttttct acagtattta aagatacccc aagaagctaa ttataacaag
    10501 acgaactcca attcactgtt ccttgcattc taaaacctta aataccagaa aacagctttt
    10561 tcaaagttgt tttcaaagtt ggcgtataac atagtatcga cggagccgat tttgaaaccg
    10621 cggtgatcac aggcagcaac gctctgtcat cgttacaatc aacatgctac cctccgcgag
    10681 atcatccgtg tttcaaaccc ggcagcttag ttgccgttct tccgaatagc atcggtaaca
    10741 tgagcaaagt ctgccgcctt acaacggctc tcccgctgac gccgtcccgg actgatgggc
    10801 tgcctgtatc gagtggtgat tttgtgccga gctgccggtc ggggagctgt tggctggctg
    10861 gtggcaggat atattgtggt gtaaacaaat tgacgcttag acaacttaat aacacattgc
    10921 ggacgttttt aatgtactga attaacgccg aattaattcc taggccacca tgttgggccc
    10981 ggcgcgcc
//
