LOCUS       sequence-300521-       10927 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Modified Tobacco rattle virus RNA2 vector that could be used for 
            expression of proteins or RNAs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10927)
  AUTHORS   Ellison EE, Nagalakshmi U, Gamo ME, Huang PJ, Dinesh-Kumar S, Voytas
            DF
  TITLE     Multiplexed heritable gene editing using RNA viruses and mobile 
            single guide RNAs.
  JOURNAL   Nat Plants. 2020 Jun 1. pii: 10.1038/s41477-020-0670-y. doi: 
            10.1038/s41477-020-0670-y.
  PUBMED    32483329
REFERENCE   2  (bases 1 to 10927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Plants.
            2020 Jun 1. pii: 10.1038/s41477-020-0670-y. doi: 
            10.1038/s41477-020-0670-y."
COMMENT     Sequence Label: SPDK3876(TRV-RNA2-pPEBV-MCS)
FEATURES             Location/Qualifiers
     source          1..10927
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        427..772
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     715..735
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             2857..2874
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      2909..3161
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    3183..3207
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4508..5137
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5566..6639
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      6705..6899
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(7135..7157)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7243..7383
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7298..7317
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7398..7415)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7569..8157)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7649..7668)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9582..10376)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    10801..10825
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 aagcttgcat gcctgcaggt caacatggtg gagcacgaca ctctcgtcta ctccaagaat
       61 atcaaagata cagtctcaga agaccagagg gctattgaga cttttcaaca aagggtaata
      121 tcgggaaacc tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta
      181 gaaaaggaag atggcttcta caaatgccat cattgcgata aaggaaaggc tatcgttcaa
      241 gatgcctcta ccgacagtgg tcccaaagat ggacccccac ccacgaggaa catcgtggaa
      301 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgatgg tcaacatggt
      361 ggagcacgac actctcgtct actccaagaa tatcaaagat acagtctcag aagaccagag
      421 ggctattgag acttttcaac aaagggtaat atcgggaaac ctcctcggat tccattgccc
      481 agctatctgt cacttcatcg aaaggacagt agaaaaggaa gatggcttct acaaatgcca
      541 tcattgcgat aaaggaaagg ctatcgttca agatgcctct accgacagtg gtcccaaaga
      601 tggaccccca cccacgagga acatcgtgga aaaagaagac gttccaacca cgtcttcaaa
      661 gcaagtggat tgatgtgata tctccactga cgtaagggat gacgcacaat cccactatcc
      721 ttcgcaagac ccttcctcta tataaggaag ttcatttcat ttggagagga taaaacattg
      781 cacctatggt gttgccctgg ctggggtatg tcagtgatcg cagtagaatg tactaattga
      841 caagttggag aatacggtag aacgtcctta tccaacacag cctttatccc tctccctgac
      901 gaggtttttg tcagtgtaat atttcttttt gaactatcca gcttagtacc gtacgggaaa
      961 gtgactggtg tgcttatctt tgaaatgtta ctttgggttt cggttcttta ggttagtaag
     1021 aaagcacttg tcttctcata caaaggaaaa cctgagacgt atcgcttacg aaagtagcaa
     1081 tgaaagaaag gtggtggttt taatcgctac cgcaaaaacg atggggtcgt tttaattaac
     1141 ttctcctacg caagcgtcta aacggacgtt ggggttttgc tagtttcttt agagaaaact
     1201 agctaagtct ttaatgttat cattagagat ggcataaata taatacttgt gtctgctgat
     1261 aagatcattt taatttggac gattagactt gttgaactac aggttactga atcacttgcg
     1321 ctaatcaaca tgggagatat gtacgatgaa tcatttgaca agtcgggcgg tcctgctgac
     1381 ttgatggacg attcttgggt ggaatcagtt tcgtggaaag atctgttgaa gaagttacac
     1441 agcataaaat ttgcactaca gtctggtaga gatgagatca ctgggttact agcggcactg
     1501 aatagacagt gtccttattc accatatgag cagtttccag ataagaaggt gtatttcctt
     1561 ttagactcac gggctaacag tgctcttggt gtgattcaga acgcttcagc gttcaagaga
     1621 cgagctgatg agaagaatgc agtggcgggt gttacaaata ttcctgcgaa tccaaacaca
     1681 acggttacga cgaaccaagg gagtactact actaccaagg cgaacactgg ctcgactttg
     1741 gaagaagact tgtacactta ttacaaattc gatgatgcct ctacagcttt ccacaaatct
     1801 ctaacttcgt tagagaacat ggagttgaag agttattacc gaaggaactt tgagaaagta
     1861 ttcgggatta agtttggtgg agcagctgct agttcatctg caccgcctcc agcgagtgga
     1921 ggtccgatac gtcctaatcc ctagggattt aaggacgtga actctgttga gatctctgtg
     1981 aaattcagag ggtgggtgat accatattca ctgatgccat tagcgacatc taaatagggc
     2041 taattgtgac taatttgagg gaatttcctt taccattgac gtcagtgtcg ttggtagcat
     2101 ttgagtttcg gcacacaagg ttaaaaacgc tgtagtaata catgcgcaag aacaggctga
     2161 gcatcttgtt ctggggtttc acactatctt tagagaaagt gttaagttaa ttaagttatc
     2221 ttaattaaga gcataattat actgatttgt ctctcgttga tagagtctat cattctgtta
     2281 ttaaaaattt gacaactcgg tttgctgacc tactggtcac tgtatcactt acccgagtta
     2341 acgagtctag aaggcctcca tggggatccg gtaccgagct cacgcgtctc gaggcccggg
     2401 catgtcccga agacattaaa ctacggttct ttaagtagat ccgtgtctga agttttaggt
     2461 tcaatttaaa cctacgagat tgacattctc gactgatctt gattgatcgg taagtctttt
     2521 gtaatttaat tttctttttg attttatttt aaattgttat ctgtttctgt gtatagactg
     2581 tttgagatcg gcgtttggcc gactcattgt cttaccatag gggaacggac tttgtttgtg
     2641 ttgttatttt atttgtattt tattaaaatt ctcaacgatc tgaaaaagcc tcgcggctaa
     2701 gagattgttg gggggtgagt aagtactttt aaagtgatga tggttacaaa ggcaaaaggg
     2761 gtaaaacccc tcgcctacgt aagcgttatt acgcccgtct gtacttatat cagtacactg
     2821 acgagtccct aaaggacgaa acgggagaac gctagccacc accaccacca ccacgtgtga
     2881 attacaggtg accagctcga atttccccga tcgttcaaac atttggcaat aaagtttctt
     2941 aagattgaat cctgttgccg gtcttgcgat gattatcata taatttctgt tgaattacgt
     3001 taagcatgta ataattaaca tgtaatgcat gacgttattt atgagatggg tttttatgat
     3061 tagagtcccg caattataca tttaatacgc gatagaaaac aaaatatagc gcgcaaacta
     3121 ggataaatta tcgcgcgcgg tgtcatctat gttactagat cgggaattaa actatcagtg
     3181 tttgacagga tatattggcg ggtaaaccta agagaaaaga gcgtttatta gaataatcgg
     3241 atatttaaaa gggcgtgaaa aggtttatcc gttcgtccat ttgtatgtgc atgccaacca
     3301 cagggttccc ctcgggatca aagtactttg atccaacccc tccgctgcta tagtgcagtc
     3361 ggcttctgac gttcagtgca gccgtcttct gaaaacgaca tgtcgcacaa gtcctaagtt
     3421 acgcgacagg ctgccgccct gcccttttcc tggcgttttc ttgtcgcgtg ttttagtcgc
     3481 ataaagtaga atacttgcga ctagaaccgg agacattacg ccatgaacaa gagcgccgcc
     3541 gctggcctgc tgggctatgc ccgcgtcagc accgacgacc aggacttgac caaccaacgg
     3601 gccgaactgc acgcggccgg ctgcaccaag ctgttttccg agaagatcac cggcaccagg
     3661 cgcgaccgcc cggagctggc caggatgctt gaccacctac gccctggcga cgttgtgaca
     3721 gtgaccaggc tagaccgcct ggcccgcagc acccgcgacc tactggacat tgccgagcgc
     3781 atccaggagg ccggcgcggg cctgcgtagc ctggcagagc cgtgggccga caccaccacg
     3841 ccggccggcc gcatggtgtt gaccgtgttc gccggcattg ccgagttcga gcgttcccta
     3901 atcatcgacc gcacccggag cgggcgcgag gccgccaagg cccgaggcgt gaagtttggc
     3961 ccccgcccta ccctcacccc ggcacagatc gcgcacgccc gcgagctgat cgaccaggaa
     4021 ggccgcaccg tgaaagaggc ggctgcactg cttggcgtgc atcgctcgac cctgtaccgc
     4081 gcacttgagc gcagcgagga agtgacgccc accgaggcca ggcggcgcgg tgccttccgt
     4141 gaggacgcat tgaccgaggc cgacgccctg gcggccgccg agaatgaacg ccaagaggaa
     4201 caagcatgaa accgcaccag gacggccagg acgaaccgtt tttcattacc gaagagatcg
     4261 aggcggagat gatcgcggcc gggtacgtgt tcgagccgcc cgcgcacgtc tcaaccgtgc
     4321 ggctgcatga aatcctggcc ggtttgtctg atgccaagct ggcggcctgg ccggccagct
     4381 tggccgctga agaaaccgag cgccgccgtc taaaaaggtg atgtgtattt gagtaaaaca
     4441 gcttgcgtca tgcggtcgct gcgtatatga tgcgatgagt aaataaacaa atacgcaagg
     4501 ggaacgcatg aaggttatcg ctgtacttaa ccagaaaggc gggtcaggca agacgaccat
     4561 cgcaacccat ctagcccgcg ccctgcaact cgccggggcc gatgttctgt tagtcgattc
     4621 cgatccccag ggcagtgccc gcgattgggc ggccgtgcgg gaagatcaac cgctaaccgt
     4681 tgtcggcatc gaccgcccga cgattgaccg cgacgtgaag gccatcggcc ggcgcgactt
     4741 cgtagtgatc gacggagcgc cccaggcggc ggacttggct gtgtccgcga tcaaggcagc
     4801 cgacttcgtg ctgattccgg tgcagccaag cccttacgac atatgggcca ccgccgacct
     4861 ggtggagctg gttaagcagc gcattgaggt cacggatgga aggctacaag cggcctttgt
     4921 cgtgtcgcgg gcgatcaaag gcacgcgcat cggcggtgag gttgccgagg cgctggccgg
     4981 gtacgagctg cccattcttg agtcccgtat cacgcagcgc gtgagctacc caggcactgc
     5041 cgccgccggc acaaccgttc ttgaatcaga acccgagggc gacgctgccc gcgaggtcca
     5101 ggcgctggcc gctgaaatta aatcaaaact catttgagtt aatgaggtaa agagaaaatg
     5161 agcaaaagca caaacacgct aagtgccggc cgtccgagcg cacgcagcag caaggctgca
     5221 acgttggcca gcctggcaga cacgccagcc atgaagcggg tcaactttca gttgccggcg
     5281 gaggatcaca ccaagctgaa gatgtacgcg gtacgccaag gcaagaccat taccgagctg
     5341 ctatctgaat acatcgcgca gctaccagag taaatgagca aatgaataaa tgagtagatg
     5401 aattttagcg gctaaaggag gcggcatgga aaatcaagaa caaccaggca ccgacgccgt
     5461 ggaatgcccc atgtgtggag gaacgggcgg ttggccaggc gtaagcggct gggttgcctg
     5521 ccggccctgc aatggcactg gaacccccaa gcccgaggaa tcggcgtgag cggtcgcaaa
     5581 ccatccggcc cggtacaaat cggcgcggcg ctgggtgatg acctggtgga gaagttgaag
     5641 gccgcgcagg ccgcccagcg gcaacgcatc gaggcagaag cacgccccgg tgaatcgtgg
     5701 caagcggccg ctgatcgaat ccgcaaagaa tcccggcaac cgccggcagc cggtgcgccg
     5761 tcgattagga agccgcccaa gggcgacgag caaccagatt ttttcgttcc gatgctctat
     5821 gacgtgggca cccgcgatag tcgcagcatc atggacgtgg ccgttttccg tctgtcgaag
     5881 cgtgaccgac gagctggcga ggtgatccgc tacgagcttc cagacgggca cgtagaggtt
     5941 tccgcagggc cggccggcat ggccagtgtg tgggattacg acctggtact gatggcggtt
     6001 tcccatctaa ccgaatccat gaaccgatac cgggaaggga agggagacaa gcccggccgc
     6061 gtgttccgtc cacacgttgc ggacgtactc aagttctgcc ggcgagccga tggcggaaag
     6121 cagaaagacg acctggtaga aacctgcatt cggttaaaca ccacgcacgt tgccatgcag
     6181 cgtacgaaga aggccaagaa cggccgcctg gtgacggtat ccgagggtga agccttgatt
     6241 agccgctaca agatcgtaaa gagcgaaacc gggcggccgg agtacatcga gatcgagcta
     6301 gctgattgga tgtaccgcga gatcacagaa ggcaagaacc cggacgtgct gacggttcac
     6361 cccgattact ttttgatcga tcccggcatc ggccgttttc tctaccgcct ggcacgccgc
     6421 gccgcaggca aggcagaagc cagatggttg ttcaagacga tctacgaacg cagtggcagc
     6481 gccggagagt tcaagaagtt ctgtttcacc gtgcgcaagc tgatcgggtc aaatgacctg
     6541 ccggagtacg atttgaagga ggaggcgggg caggctggcc cgatcctagt catgcgctac
     6601 cgcaacctga tcgagggcga agcatccgcc ggttcctaat gtacggagca gatgctaggg
     6661 caaattgccc tagcagggga aaaaggtcga aaaggtctct ttcctgtgga tagcacgtac
     6721 attgggaacc caaagccgta cattgggaac cggaacccgt acattgggaa cccaaagccg
     6781 tacattggga accggtcaca catgtaagtg actgatataa aagagaaaaa aggcgatttt
     6841 tccgcctaaa actctttaaa acttattaaa actcttaaaa cccgcctggc ctgtgcataa
     6901 ctgtctggcc agcgcacagc cgaagagctg caaaaagcgc ctacccttcg gtcgctgcgc
     6961 tccctacgcc ccgccgcttc gcgtcggcct atcgcggccg ctggccgctc aaaaatggct
     7021 ggcctacggc caggcaatct accagggcgc ggacaagccg cgccgtcgcc actcgaccgc
     7081 cggcgcccac atcaaggcac cctgcctcgc gcgtttcggt gatgacggtg aaaacctctg
     7141 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
     7201 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc
     7261 acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca gattgtactg
     7321 agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
     7381 aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     7441 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     7501 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     7561 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     7621 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     7681 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     7741 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     7801 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     7861 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     7921 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     7981 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
     8041 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     8101 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     8161 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     8221 ctgatgaatc ccctaatgat ttttatcaaa atcattaagt taaggtagat acacatcttg
     8281 tcatatgatc aaatggtttc gccaaaaatc aataatcaga caacaaaatg tgcgaactcg
     8341 atattttaca cgactctctt taccaattct gccccgaatt acacttaaaa cgactcaaca
     8401 gcttaacgtt ggcttgccac gccttacttg actgtaaaac tctcactctt accgaacttg
     8461 gccgtaacct gccaaccaaa gcgagaacaa aacataacat caaacgaatc gaccgattgt
     8521 taggtaatcg tcacctccac aaagagcgac tcgctgtata ccgttggcat gctagcttta
     8581 tctgttcggg caatacgatg cccattgtac ttgttgactg gtctgatatc cgtgagcaaa
     8641 aacggcttat ggtattgcga gcttcagtcg cactacacgg tcgttctgtt actctttatg
     8701 agaaagcgtt cccgctttca gagcaatgtt caaagaaagc tcatgaccaa tttctagccg
     8761 accttgcgag cattctaccg agtaacacca caccgctcat tgtcagtgat gctggcttta
     8821 aagtgccatg gtataaatcc gttgagaagc tgggttggta ctggttaagt cgagtaagag
     8881 gaaaagtaca atatgcagac ctaggagcgg aaaactggaa acctatcagc aacttacatg
     8941 atatgtcatc tagtcactca aagactttag gctataagag gctgactaaa agcaatccaa
     9001 tctcatgcca aattctattg tataaatctc gctctaaagg ccgaaaaaat cagcgctcga
     9061 cacggactca ttgtcaccac ccgtcaccta aaatctactc agcgtcggca aaggagccat
     9121 ggattctagc aactaactta cctgttgaaa ttcgaacacc caaacaactt gttaatatct
     9181 attcgaagcg aatgcagatt gaagaaacct tccgagactt gaaaagtcct gcctacggac
     9241 taggcctacg ccatagccga acgagcagct cagagcgttt tgatatcatg ctgctaatcg
     9301 ccctgatgct tcaactaaca tgttggcttg cgggcgttca tgctcagaaa caaggttggg
     9361 acaagcactt ccaggctaac acagtcagaa atcgaaacgt actctcaaca gttcgcttag
     9421 gcatggaagt tttgcggcat tctggctaca caataacaag ggaagactca ctcgtggctg
     9481 caaccctgct tactcaaaat ctattcacac atggttacgt tttggggaaa ttatgagggg
     9541 atctctcagc gttaagggat tttggtcatg cattctaggt actaaaacaa ttcatccagt
     9601 aaaatataat attttatttt ctcccaatca ggcttgatcc ccagtaagtc aaaaaatagc
     9661 tcgacatact gttcttcccc gatatcctcc ctgatcgacc ggacgcagaa ggcaatgtca
     9721 taccacttgt ccgccctgcc gcttctccca agatcaataa agccacttac tttgccatct
     9781 ttcacaaaga tgttgctgtc tcccaggtcg ccgtgggaaa agacaagttc ctcttcgggc
     9841 ttttccgtct ttaaaaaatc atacagctcg cgcggatctt taaatggagt gtcttcttcc
     9901 cagttttcgc aatccacatc ggccagatcg ttattcagta agtaatccaa ttcggctaag
     9961 cggctgtcta agctattcgt atagggacaa tccgatatgt cgatggagtg aaagagcctg
    10021 atgcactccg catacagctc gataatcttt tcagggcttt gttcatcttc atactcttcc
    10081 gagcaaagga cgccatcggc ctcactcatg agcagattgc tccagccatc atgccgttca
    10141 aagtgcagga cctttggaac aggcagcttt ccttccagcc atagcatcat gtccttttcc
    10201 cgttccacat cataggtggt ccctttatac cggctgtccg tcatttttaa atataggttt
    10261 tcattttctc ccaccagctt atatacctta gcaggagaca ttccttccgt atcttttacg
    10321 cagcggtatt tttcgatcag ttttttcaat tccggtgata ttctcatttt agccatttat
    10381 tatttccttc ctcttttcta cagtatttaa agatacccca agaagctaat tataacaaga
    10441 cgaactccaa ttcactgttc cttgcattct aaaaccttaa ataccagaaa acagcttttt
    10501 caaagttgtt ttcaaagttg gcgtataaca tagtatcgac ggagccgatt ttgaaaccgc
    10561 ggtgatcaca ggcagcaacg ctctgtcatc gttacaatca acatgctacc ctccgcgaga
    10621 tcatccgtgt ttcaaacccg gcagcttagt tgccgttctt ccgaatagca tcggtaacat
    10681 gagcaaagtc tgccgcctta caacggctct cccgctgacg ccgtcccgga ctgatgggct
    10741 gcctgtatcg agtggtgatt ttgtgccgag ctgccggtcg gggagctgtt ggctggctgg
    10801 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
    10861 gacgttttta atgtactgaa ttaacgccga attaattcct aggccaccat gttgggcccg
    10921 gcgcgcc
//
