LOCUS       sequence-300524-       13217 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Halothiobacillus neapolitanus carboxysome operon with full length 
            CsoS2. For heterologous expression of carboxysomes in E. coli..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13217)
  AUTHORS   Oltrogge LM, Chaijarasphong T, Chen AW, Bolin ER, Marqusee S, Savage
            DF
  TITLE     Multivalent interactions between CsoS2 and Rubisco mediate 
            alpha-carboxysome formation.
  JOURNAL   Nat Struct Mol Biol. 2020 Mar;27(3):281-287. doi: 
            10.1038/s41594-020-0387-7. Epub 2020 Mar 2.
  PUBMED    32123388
REFERENCE   2  (bases 1 to 13217)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41594-020-0387-7"; journalName: "Nat Struct Mol Biol"; 
            date: "2020-03"; volume: "27"; issue: "3"; pages: "281-287"
COMMENT     Sequence Label: pLz37_CB_operon_CsoS2_N1-N4
FEATURES             Location/Qualifiers
     source          1..13217
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1..28
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     protein_bind    50..69
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     promoter        join(84..89,90..107,108..114)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    122..138
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             8632..9291
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(8698..8717)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        9356..9433
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             9434..10516
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(9453..9472)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     rep_origin      complement(11498..12086)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11578..11597)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      13040..13126
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 agaaggccat cctgacggat ggcctttttg cgtttctact ctagtttcga attgtgagcg
       61 ctcacaattt cgaaacccca ggcttgacac tttatgcttc cggctcgtat aatgtgtgga
      121 attgtgagcg gataacaatt tcagctaggt gcgcgaatcc cccatccttt caggaggaac
      181 tcatggcagt taaaaagtat agtgctggtg taaaagaata ccggcagacc tattggatgc
      241 cggaatacac accgttggat tccgacatcc ttgcatgctt caaaatcacc ccacaaccgg
      301 gtgttgatcg cgaagaagcc gcagccgcgg ttgcagcaga atcttcaacc ggcacatgga
      361 ccaccgtgtg gaccgatttg ctgaccgaca tggactacta caaaggccgt gcctatcgca
      421 ttgaagacgt acccggtgac gatgcggcat tctatgcctt tatcgcctac ccaatcgacc
      481 tgttcgaaga agggtcagtt gttaacgtgt ttacctcact ggttggtaac gtgttcggct
      541 tcaaagcggt acgcggcctg cgtctggaag atgttcgctt cccactcgcc tacgttaaaa
      601 cctgtggcgg cccaccgcac ggtattcaag tcgaacgcga caagatgaac aaatatggtc
      661 gcccactgtt gggttgcacc atcaagccaa aacttggttt gtctgcgaaa aactacggcc
      721 gtgccgtata cgagtgcctc cgtggcggcc tcgacttcac taaagatgat gaaaacatca
      781 attctcagcc gttcatgcgc tggcgcgatc gcttcttgtt cgtacaagac gcgaccgaaa
      841 ctgctgaagc ccaaaccggc gaacgcaaag gccattacct caacgtaacg gcgccaactc
      901 ctgaagaaat gtacaagcgc gcagaattcg ccaaagaaat tggcgcgcca atcattatgc
      961 acgactacat caccggtggc ttcacggcca acactggctt ggccaagtgg tgtcaagaca
     1021 acggcgtact gctgcacatc caccgtgcga tgcatgcggt tatcgaccgt aacccgaacc
     1081 acggtattca cttccgtgtt ctgaccaaga ttctgcgttt gtcgggtggc gatcacctgc
     1141 acaccggtac cgttgtcggc aaactggaag gcgaccgtgc ctctactctg ggctggattg
     1201 atttgctccg cgaatcgttt atccctgaag atcgctcgcg cggtatcttc ttcgatcaag
     1261 actggggttc aatgccaggc gtattcgctg tggcctctgg tggtattcac gtatggcaca
     1321 tgcctgcgct ggttaacatc tttggtgacg actctgtcct ccaattcggt ggcggtacgc
     1381 tgggtcatcc atggggcaac gctgccggtg ctgctgccaa ccgtgttgct ctggaagcct
     1441 gcgtagaagc gcgtaaccaa ggccgcgata tcgaaaaaga aggcaaagaa attctgactg
     1501 ctgctgcaca gcacagccca gaactgaaga ttgccatgga aacttggaaa gagatcaaat
     1561 tcgaatttga cactgtcgac aaactcgaca ctcaaaatcg ttgatccctc gtaccacaca
     1621 acatactaag gtgagtaacc atggctgaaa tgcaggatta caagcaaagc ctcaaatatg
     1681 agactttctc ttatcttcca cccatgaacg cggaacgcat ccgcgctcaa atcaagtacg
     1741 caattgctca aggctggagc cccggcattg agcacgtaga agtgaaaaac tccatgaacc
     1801 aatattggta catgtggaaa cttcccttct tcggcgaaca aaatgtcgac aacgtgttgg
     1861 ctgaaattga agcgtgtcgt agtgcgtatc caacacacca ggtcaaactg gtggcttatg
     1921 acaactatgc gcaaagctta ggtctggcct tcgtggtcta ccgcggcaac taagtcaagt
     1981 ctgtcattgc gctgtgcgtt tctctacgca cagcacttat tcaagatggg gtcaaacatg
     2041 ccttcacagt caggaatgaa tcctgccgac ctgagcggac tctctggcaa ggaactggca
     2101 cgcgcacgcc gcgctgcact atccaagcaa gggaaagcag ctgtttctaa taaaacggct
     2161 agcgtaaacc gtagcactaa acaggcggca tcttcgatca atacaaatca ggtgcgctct
     2221 tctgtaaatg aagtgcccac tgattaccaa atggcggatc aattgtgctc tacgattgat
     2281 catgctgact ttggtaccga aagcaatcgc gttagagatc tctgccgtca acgcagagag
     2341 gcactatcaa ctatcggtaa aaaagcggtt aaaaccaacg gcaagccgtc gggtcgcgtt
     2401 cgaccacagc aatcagtggt tcacaacgac gcaatgatcg aaaatgccgg tgatactaac
     2461 caatcatcgt ccacttcatt aaataatgaa ctttccgaaa tctgctccat agcagacgac
     2521 atgccggagc gttttggttc acaagccaaa accgtccgtg atatctgccg tgcacgccgt
     2581 caagcgctct ctgagcgtgg aactcgcgcc gtgccgccaa agccgcaatc tcaaggtggt
     2641 ccaggacgca atggctatca aattgatgga tacctagata ccgcacttca tggccgcgat
     2701 gccgccaagc gccaccgtga aatgctctgt caatacggcc gcggcacagc accttcctgc
     2761 aagccaacag gccgtgtcaa aaattctgta cagtcgggca acgcagcgcc aaaaaaggtt
     2821 gaaaccggtc acaccctatc cggcggatct gttacgggca cgcaagtgga tcgtaaatct
     2881 catgtgactg gaaacgagcc gggcacttgc cgagcagtca cgggcaccga gtacgtaggt
     2941 actgagcaat tcacctcttt ttgcaatacc agccccaagc caaatgcgac gaaggtcaat
     3001 gtgaccacaa cggctcgtgg tcgccctgtt agcggtacgg aagtttcacg gaccgagaag
     3061 gtaactggca acgaatccgg tgtctgccgt aacgttaccg gcaccgaata catgagtaat
     3121 gaagctcact tttctctatg tggaacagcc gcaaagcctt cacaagcgga taaagtcatg
     3181 ttcggcgcca cagcacgaac gcatcaagtg gtcagtggca gtgatgaatt caggccctct
     3241 tctgttacgg gtaacgaatc gggtgcaaaa cgcacaatta ccggctcgca gtacgcagac
     3301 gaaggtcttg cgcgactcac gatcaacgga gcacctgcaa aagtagccag aacccacacc
     3361 tttgcgggct ctgacgttac cggcacggaa atcggtcgct ctactcgcgt aactggtgat
     3421 gaaagcggtt cgtgtcgttc aatctcaggc accgagtatc tcagtaacga gcaattccaa
     3481 tccttttgtg acacaaaacc tcaacgcagc ccgttcaagg ttggccaaga tcgcacgaac
     3541 aagggtcagt ctgtgactgg taacttggtt gatcgttccg aactggttac aggtaacgaa
     3601 ccaggttcat gctctcgggt tacagtctct cagtatggcc aaagcaaaat ctgcggtggt
     3661 ggcgtgggaa aagtgcgctc aatgcgcacc cttcgcggca cctcagtatc tggccaacag
     3721 ctagatcatg ccccaaagat gtccggtgac gagcgcggcg ggtgcatgcc cgtcaccggt
     3781 aatgagtact acggtcgtga acatttcgaa ccgttttgta cgagcacccc agagcccgaa
     3841 gctcaatcaa ctgaacaatc attgacctgt gaaggacaaa ttattagcgg cacttcagtt
     3901 gacgccagtg atttggtcac aggaaatgaa atcggtgaac agcaactcat cagcggtgac
     3961 gcctatgttg gcgcgcagca gacaggttgc cttcccacta gtccacgctt caaccaaact
     4021 ggcaatgttc agtcaatggg ttttaagaac accaatcagc cagaacaaaa ctttgcacca
     4081 ggtgaagtaa tgcctactga ctttagtatt caaaccccag ctcgctcggc tcagaatcgc
     4141 attacaggta acgacattgc gccctcaggt cgcattacag gccctggtat gctggcaacc
     4201 ggcttgatta caggaacccc cgaattcagg cacgctgcgc gcgagttggt tggttctcca
     4261 caacccatgg caatggccat ggccaaccgt aataaggctg ctcaagcacc tgttgtgcag
     4321 ccagaagtgg ttgcaactca ggaaaagcct gagttggtat gtgcaccaag aagcgatcaa
     4381 atggatcgtg tgagtggcga aggcaaagaa cgttgccaca tcactggcga tgactggtca
     4441 gttaacaagc acatcaccgg tacagccggt caatgggcga gtggtcgcaa cccttccatg
     4501 cgcggtaatg cgcgtgtggt cgaaaccagc gcgtttgcca atcgcaatgt gccaaaacct
     4561 gaaaagccgg gctccaagat cacgggcagt agtggtaatg acacccaagg tagtctgatc
     4621 acttactccg gcggcgcgcg cggttgagcg ttaagtaaag tgtaacgatc atgaacaccc
     4681 gtaacacacg aagcaagcaa cgcgcaccgt ttggtgttag ctcatcagtc aaacctcggc
     4741 ttgacttgat tgagcaagca ccaaaccctg cctatgaccg ccatcctgct tgcataacgt
     4801 tgcctgagcg tacctgccgg cacccgttaa ctgaccttga agccaacgaa caactgggtc
     4861 gttgcgagga tagcgtcaag aaccgttttg atcgcgttat ccctttcttg caagttgttg
     4921 ctggcattcc tcttggtctg gattatgtta cccgtgttca agaattagcc cagtcgtcgc
     4981 ttggacatac gctgcccgaa gaattactca aagataattg gatcagtggg cacaatttaa
     5041 aaggcatttt tggctacgcg actgctaaag cactaacagc agccacggaa caattcagtc
     5101 gtaaaataat gtctgagaag gacgattccg catcggccat tggcttcttc ctggattgcg
     5161 ggttccacgc agtggacata agcccttgtg ccgatggtcg tctcaaaggg ttactgcctt
     5221 acatattgcg tttgcccctt acggcattca cctatcgtaa agcctacgca ggctcgatgt
     5281 tcgatattga agatgatctg gcacagtggg agaaaaatga actccgccgt tatcgtgaag
     5341 gtgttccaaa tacagcggat cagccaacac gatacctgaa aattgctgtg tatcatttca
     5401 gcacctctga cccgacacac tctggctgcg cggcacacgg cagtaatgat cgtgcagcac
     5461 tggaagcggc tttaacccag ctgatgaaat tcagagaagc ggttgaaaat gcccattgct
     5521 gcggcgcaag tatcgatatt ttactgattg gcgttgatac ggatacggat gccattcgcg
     5581 ttcatattcc ggatagcaaa ggttttttga atccgtatcg ttatgttgac aacacagtaa
     5641 cttatgcgca aacactacat ctggcgccgg atgaggctcg tgtgattatt cacgaagcaa
     5701 ttctcaacgc aaaccgcagc gatggttggg ctaaaggaaa tggagtagcc agcgagggga
     5761 tgcgtcgttt tattggtcag cttttgatca acaacctctc gcaaatcgat tacgtagtaa
     5821 atcgtcatgg tggtcgctat ccacccaatg atattggtca tgctgagcga tatatcagtg
     5881 ttggtgatgg ttttgatgaa gttcaaatcc ggaatttagc ctactacgcg catttggata
     5941 cggttgaaga aaatgcgatt gatgtggatg tgggaatcaa aattttcacc aaacttaatt
     6001 tgagtcgagg tttaccgatt ccgattgcca tccactatcg ctatgacccc aatgttccag
     6061 gctccagaga aagaaccgtg gtcaaagcaa gacggatata caacgccatt aaagagcggt
     6121 tctcatcctt ggatgagcag aatctattgc agtttcgttt gagcgttcag gcgcaggata
     6181 tcggaagccc gattgaagag gttgcatccg catgaaaatc atgcaagttg agaaaacgtt
     6241 ggtttcaaca aaccgtattg ctgatatggg tcacaaacca ctattagtgg tatgggagaa
     6301 accgggcgcg cccaggcagg tcgccgtgga tgcgattggc tgcataccgg gcgattgggt
     6361 tttgtgcgtt gggtcatcgg cagcacgaga ggctgcagga agcaagtctt atccctctga
     6421 tttgacgatt atcgggatta ttgatcagtg gaatggtgag taatggaagt aatgcgcgtt
     6481 cgttccgacc taatcgcaac acgcaggatt cccggtctta aaaatatctc tttgcgtgtc
     6541 atggaggatg ctacgggtaa ggtcagtgtc gcttgcgatc ccattggcgt tcccgaggga
     6601 tgttgggtct ttacgattag cggctctgcc gctcggtttg gcgtgggtga ttttgagatt
     6661 ctcacggatt tgacgattgg tggcatcatc gatcactggg taacttgacc atcgctagat
     6721 gagttgattt tgaatgagtc tttattgagg agagaagaaa tggcagcagt aacaggtatt
     6781 gcactgggta tgattgaaac acgtggtctg gttccagcga ttgaagctgc cgatgccatg
     6841 accaaggccg ccgaagtacg tttggttggc cgtcaatttg ttggtggtgg ttacgtgacc
     6901 gttttggtcc gtggtgaaac cggtgccgtc aacgcagcag ttcgtgcggg cgctgatgcc
     6961 tgcgaacgag tcggcgatgg tctggtcgcg gcgcatatca ttgcccgtgt ccattccgaa
     7021 gtcgaaaaca tcctgccgaa agcccctgaa gcttaaggat tgggaaagac gaaccggcgc
     7081 aggcttgttc cggttctttg cataaagtga cagcttagga gtttatttaa atggctgatg
     7141 taactggtat tgctctgggt atgatcgaaa cacgtggctt agttcctgcg attgaagcag
     7201 cggacgccat gactaaagcg gctgaagtgc gtttggtcgg tcgtcaattt gttggtggcg
     7261 gttacgtcac cgtattggtt cggggcgaaa caggcgctgt taacgccgct gttcgtgctg
     7321 gcgccgatgc ttgcgaacgt gttggtgatg gcttggttgc tgcgcacatc attgcgcgtg
     7381 tccactcaga agtagaaaac attctgccta aggcgccaca agcctaagtc agatattcct
     7441 aagacggctc acttcggcga cacccgtcgc cgaaaccgtc taaccaaatt tctgggctcg
     7501 ttctgaacgt gctcaacact agttttagag gatctgttat ggcaacgact cacggtattg
     7561 ccctgggcat gattgaaaca cgaggattgg ttcctgccat tgaagccgca gatgccatga
     7621 ccaaagcggc ggaagtccgt ctggtcggac gatcatttgt tggcggcggt tacgtgaccg
     7681 taatggttcg tggtgagaca ggtgcagtaa atgctgccgt tcgtgcgggt gctgacgcct
     7741 gtgaacgtgt tggcgatggc ctggttgctg cgcacatcat tgcgcgcgtt cattctgaag
     7801 ttgagatcat cctacccgag acgcccgaag actcagattc cgcgtggtgt atcgcaaatc
     7861 tgaatagcta atgtctagta gggaagatgc gcatgaacaa cattgatttg cgcgtctatt
     7921 cgtttatcga ttcgcttcaa ccgcagcttg catcctatct tgcgacatca tcgcaaggct
     7981 ttcttcccgt tccgggtgat gcctgcttgt ggattgaagt cgcgccgggc atggccgtcc
     8041 atcgcctcag tgatattgcg ctaaaggcca cgaacgttcg tctcggcgaa caggtagtcg
     8101 agcgtgcttt tggctcgatg gaaattcatt accgaaacca aagcgacgtt ctcgcatccg
     8161 gtgaggccgt tttaagagaa atcaatcacg cgcaagaaga tcgtctgcct tgtcgcatcg
     8221 catggaaaga gatcattcga gcgattaccc ccgatcatgc caccttgatc aatcgccagt
     8281 tgcgtaaagg ctctatgctg ttgccgggca aaagcatgtt catccttgaa acagaaccgg
     8341 caggttatat tgttcaggct gccaacgagg ccgagaaagc agctcatgtg actctgatcg
     8401 atgtacgtgc ttttggtaac tttggtcgcc tgaccatgat gggcagcgaa gcggaaaccg
     8461 aagaagccat gcgggcggct gaggccacaa tcgcaagcat caacgcgcgt gcgcgtcgcg
     8521 ctgaagggtt ctaagcggcc gcgaggttcc aactttcacc ataatgaaat aagatcacta
     8581 ccgggcgtat tttttgagtt atcgagattt tcaggagcta aggaagctaa aatggagaaa
     8641 aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga acattttgag
     8701 gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga tattacggcc
     8761 tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat tcacattctt
     8821 gcccgcctga tgaatgctca tccggagttc cgtatggcaa tgaaagacgg tgagctggtg
     8881 atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga aacgttttca
     8941 tcgctctgga gtgaatacca cgacgatttc cggcagtttc tacacatata ttcgcaagat
     9001 gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga gaatatgttt
     9061 ttcgtctcag ccaatccctg ggtgagtttc accagttttg atttaaacgt ggccaatatg
     9121 gacaacttct tcgcccccgt tttcactatg ggcaaatatt atacgcaagg cgacaaggtg
     9181 ctgatgccgc tggcgattca ggttcatcat gccgtttgtg atggcttcca tgtcggcaga
     9241 atgcttaatg aattacaaca gtactgcgat gagtggcagg gcggggcgta atttttttaa
     9301 ggcagttatt ggtgcccttg aattcctact agtcgaagcg gcatgcattt acgttgacac
     9361 catcgaatgg tgcaaaacct ttcgcggtat ggcatgatag cgcccggaag agagtcaatt
     9421 cagggtggtg aatgtgaaac cagtaacgtt atacgatgtc gcagagtatg ccggtgtctc
     9481 ttatcagacc gtttcccgcg tggtgaacca ggccagccac gtttctgcga aaacgcggga
     9541 aaaagtggaa gcggcgatgg cggagctgaa ttacattccc aaccgcgtgg cacaacaact
     9601 ggcgggcaaa cagtcgttgc tgattggcgt tgccacctcc agtctggccc tgcacgcgcc
     9661 gtcgcaaatt gtcgcggcga ttaaatctcg cgccgatcaa ctgggtgcca gcgtggtggt
     9721 gtcgatggta gaacgaagcg gcgtcgaagc ctgtaaagcg gcggtgcaca atcttctcgc
     9781 gcaacgcgtc agtgggctga tcattaacta tccgctggat gaccaggatg ccattgctgt
     9841 ggaagctgcc tgcactaatg ttccggcgtt atttcttgat gtctctgacc agacacccat
     9901 caacagtatt attttctccc atgaagacgg tacgcgactg ggcgtggagc atctggtcgc
     9961 attgggtcac cagcaaatcg cgctgttagc gggcccatta agttctgtct cggcgcgtct
    10021 gcgtctggct ggctggcata aatatctcac tcgcaatcaa attcagccga tagcggaacg
    10081 ggaaggcgac tggagtgcca tgtccggttt tcaacaaacc atgcaaatgc tgaatgaggg
    10141 catcgttccc actgcgatgc tggttgccaa cgatcagatg gcgctgggcg caatgcgcgc
    10201 cattaccgag tccgggctgc gcgttggtgc ggatatctcg gtagtgggat acgacgatac
    10261 cgaagacagc tcatgttata tcccgccgtt aaccaccatc aaacaggatt ttcgcctgct
    10321 ggggcaaacc agcgtggacc gcttgctgca actctctcag ggccaggcgg tgaagggcaa
    10381 tcagctgttg cccgtctcac tggtgaaaag aaaaaccacc ctggcgccca atacgcaaac
    10441 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
    10501 ggaaagcggg cagtgatccc acagccgcca gttccgctgg cggcatttta actttcttta
    10561 ataatctagt gacaagccgg ggcagacgtg agccgtagtc ccgtcgccag acgcgggtgc
    10621 ccacgggcgt cgtcaggatg tccgtaattg actgccggag gtggtcaatg cccttcagct
    10681 ccttgccact gtcacggctc atgcctcggg tcattagtcg cccgctccgg tatcttcact
    10741 ggcttcgatg attgccgccc cgcagctgca gaggtcaccg atccgagcag tcggcctctg
    10801 gttggtaaag accgtgcgac tgccggtgat gatcgtgttc aagccatgca gggggcaggc
    10861 gtgaaggtca tcctttcggg ccacggggcg gctgttgacg aaggtgtcgt cgctcccggt
    10921 gatgatgatc ccgccgtgat cggtcacgtc gtttagtcga gcgatgcctg gcgtcgtagt
    10981 cacgggttta ggtcaatacg acttgcggtc actgtaacgt tgccctcggc ggtcacgtta
    11041 acgtcgcctt gggcttcgac ttgcgcctcc tgcacaagga tcacaatccg tccttgggct
    11101 gcggtgaggt cgatcttgta ctcatgcgct tcgcggtcgt actggatgat tgagtcatcc
    11161 tcgaactgcg tcttttggat cgtttctttg tcctcgatct gggggtagtc agtcgagaac
    11221 gcgccgggca tcgcgaagcc ctgactgatc tcgccggagg gggccatcac gacgacggcc
    11281 tcaccgacct cgggcgccca ccagaaccga tccttgcccg ctcgctgcgt gagccacgga
    11341 atccagtcag tgaggagcag cgcctcgccg ctctcctcgt cttcctcgat cgcgacacgg
    11401 atcagcccct tgggatagtc agcctcggct accctgccta cgcggagcaa gttgccgtga
    11461 cgccgactgt ctcgagtcta gtcgcgttgc tggcgttttt ccataggctc cgcccccctg
    11521 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
    11581 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
    11641 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
    11701 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
    11761 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
    11821 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
    11881 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
    11941 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
    12001 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
    12061 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
    12121 ctcagtctag ttcagccagc tcgtcgtgat gtcgaaaccc aagccaccct cagaggtgaa
    12181 ggccgcttcg agcacatcgg gaagcgtgtc gacgacggtg ctcggtgcct cgggtaagag
    12241 ccagatagat gcggtgacgt tcaccgtcgc gatcgtggcg ggaaccacgg tcactgcgtc
    12301 cgttacaact cgcacgtcgt cggccagcac gactgactcg acggcttcga ttagcccggg
    12361 agaggcaagg ccatcaggct cggtagacag aatgctgatt aacacctcgc cgggagcagg
    12421 gctgctcaca gccgcgtcct tcacccgtgg gtcagccgtc agcgcttgat agcggtacca
    12481 ggccgcaccg cctgcggtcg agctgccctt gatccgctcg atggtgcgat cgcgaagctc
    12541 gccatccggc tcattcggct gccgcaccac gccgtagaac gcggcaaggt tgtcgaggtc
    12601 ggggccgttt gagtagcgaa gcagtgtcgc aagaagtgcg tcgttgatcc gctgacgcag
    12661 gatcagctcg cgggctgcgc agacctccag cagcttgatg accgggtcgg attcgaggat
    12721 ggcggtgtag ctggcgtcac gcgatcgcag gtcgtcgatc aagtcctgca ggatcagttc
    12781 aaagtccagc gcttcgatga tggtgggcgc gggaatcgta gcaaagtcaa gaacggtcat
    12841 gagacgacta agccctccag cgtgatacgc ctgccctcgg ggatgtagta gccgatcagg
    12901 ttcagctcga cttgaccagc tgcgctggct gagacgatgc gaaccttctc cagcttcagc
    12961 cgtggctccc agcgatcgag cgcttcagct gtggccgcca ccaggtcaac gatgagggac
    13021 tggttgatcg gctagtcatc aaataaaacg aaaggctcag tcggaagact gggcctttcg
    13081 ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc cgggagcgga
    13141 tttgaacgtt gcgaagcaac ggcccggagg gtggcgggca ggacgcccgc cataaactgc
    13201 caggcatcaa attaagc
//
