LOCUS       sequence-300565-       13865 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human SMCHD1 Exon1-46.47A-48 with a C-terminal 3xFLAG tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13865)
  AUTHORS   Hiramuki Y, Tapscott SJ
  TITLE     Identification of SMCHD1 domains for nuclear localization, 
            homo-dimerization, and protein cleavage.
  JOURNAL   Skelet Muscle. 2018 Aug 2;8(1):24. doi: 10.1186/s13395-018-0172-z.
  PUBMED    30071896
REFERENCE   2  (bases 1 to 13865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Skelet 
            Muscle."; date: "2018-08-2"; volume: "8(1):24. doi"; pages: " 
            10.1186/s13395-018-0172-z"
COMMENT     Sequence Label: pLenti-CMV-SMCHD1-Exon1-46.47A-48-3xFLAG
FEATURES             Location/Qualifiers
     source          1..13865
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    19..135
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        158..461
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        462..665
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     616..636
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     662..686
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             6667..6732
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    6742..7330
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6795..6815)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7213..7224)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        7344..7843
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7379..7402)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     7776..7795
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             7864..8463
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7864..7883)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8360..8380
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             8601..8834
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8906..9027
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8943..8962)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8997..9016
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9067..9202
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9222..9241)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9223..9241)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9251..9268)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9251..9267)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9260..9282)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9409..9864
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9496..9515)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9706..9727
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9890..9994
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9995..10063,10064..10855)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10213..10232)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11026..11614
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11515..11534
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11768..11785
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11902..11923
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11938..11943,11944..11961,11962..11968)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11976..11992
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11981..12003
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12000..12016
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12000..12016
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12035..12055
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12037..12055
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        12083..12309
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             12310..12490
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    12537..12662
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    13155..13388
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             13573..13617
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 tcgacggtat cggttaactt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
       61 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
      121 aaaattcaaa attttatcga taagcttggg agttccgcgt tacataactt acggtaaatg
      181 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      241 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      301 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      361 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      421 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      481 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      541 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      601 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      661 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
      721 atagaagaca ccgactctag aggatccatg gcagcggcgg acggcggcgg gcctggtggg
      781 gcctctgtgg ggactgagga ggatggcgga ggcgtcggcc acaggacggt gtacttgttt
      841 gatcggcgcg aaaaggagtc cgagctcggg gaccggcctc tgcaggtcgg ggagcgctcg
      901 gactacgcgg gatttcgcgc gtgtgtgtgt cagacacttg gcatttcacc tgaagaaaaa
      961 tttgttatta caacaacaag taggaaagaa attacctgtg ataattttga tgaaactgtt
     1021 aaagatggag tcaccttata cctgctacag tcggtcaatc agttactact gacagctacg
     1081 aaagaacgaa ttgacttctt acctcactat gacacactgg ttaaaagtgg catgtatgaa
     1141 tattatgcca gtgaaggaca aaatcctttg ccatttgctc ttgcggaatt aattgacaat
     1201 tcattgtctg ctacttctcg taacattggg gttagaagaa tacagatcaa attgcttttt
     1261 gatgaaacac aaggaaaacc tgctgttgca gtgatagata atggaagagg aatgacctct
     1321 aaacagctta acaactgggc cgtgtatagg ttgtcaaaat tcacaaggca aggtgacttt
     1381 gaaagtgatc attcaggata tgttcgtcca gtaccagtgc cacgcagttt aaatagtgat
     1441 atttcctatt ttggtgttgg gggcaagcaa gctgtcttct ttgttggaca atcagccaga
     1501 atgataagca aacctgcaga ttcccaagat gttcacgagc ttgtgctttc taaagaagat
     1561 tttgagaaga aggagaaaaa taaagaggca atatatagtg gatatattag aaacagaaag
     1621 ccctctgatt ctgttcacat tacaaatgat gatgaaagat ttctacatca tcttatcata
     1681 gaggagaagg aaaaagatag ctttactgct gtggttatca caggggtaca accagaacac
     1741 atacagtact tgaaaaatta tttccacctt tggacacgac agttagcgca tatttatcac
     1801 tactatattc atggcccaaa aggaaatgaa atacgaacat caaaagaagt tgaacctttc
     1861 aacaatattg atattgaaat ttctatgttt gaaaaaggga aggtacctaa gattgtcaac
     1921 ctaagggaaa tacaagacga catgcagacg ttgtatgtaa acacagcagc tgatagtttt
     1981 gaattcaaag ctcatgttga aggagatggt gtagtggaag ggattatccg ttatcatcca
     2041 ttcttatatg atagagaaac ttaccctgat gatccatgct ttccatcaaa attaaaagat
     2101 gaagatgatg aagatgattg tttcatactt gagaaagcag ctagagggaa aaggcctatt
     2161 tttgaatgtt tttggaatgg acgattaata ccatatacat cagttgaaga ctttgactgg
     2221 tgtactcctc ctaagaagag agggcttgca ccaattgaat gctacaatag gatatctggt
     2281 gcattattca ctaatgacaa attccaggtc agcacaaata aattgacgtt tatggatctt
     2341 gagctaaaat tgaaagataa gaacaccctt tttacaagga ttttaaatgg acaggaacag
     2401 cgaatgaaaa ttgacagaga atttgctttg tggctgaagg actgtcatga gaagtatgat
     2461 aaacaaataa aatttacact ttttaaggga gtaattacac gtcctgatct tccttctaaa
     2521 aagcaaggtc cctgggcaac atatgcagca atagaatggg atggaaagat atacaaagca
     2581 ggacagctgg tcaagacaat caagacactt cccctctttt atggaagcat agtaagattt
     2641 tttctttatg gcgatcatga tggagaagta tatgctacag gaggagaggt tcaaattgca
     2701 atggaacctc aggcactata tgatgaagta agaactgtgc caattgcaaa gctggatagg
     2761 acagttgctg agaaagctgt taaaaaatat gtagaagatg aaatggcaag gctccctgat
     2821 agattgtcag taacttggcc tgaaggagat gaattattgc ctaatgaggt taggcctgct
     2881 ggaaccccta ttggtgcgtt aagaattgaa atactgaata aaaaagggga agcaatgcaa
     2941 aagcttccag gaacaagcca tggagggtca aagaaactcc tggttgagct caaagttatt
     3001 ttacattctt caagtggaaa taaagagatt atttcgcata ttagtcaaca tggaggaaaa
     3061 tggccttact ggtttaaaaa aatggaaaat attcagaagt tggggaatta taccttgaaa
     3121 ttacaagttg tgttgaatga aagtaatgca gacacttatg caggaagacc actaccatct
     3181 aaagcaatta agttttctgt taaagagggt aagccagaga aattttcatt tggtcttctg
     3241 gatcttcctt ttcgtgttgg agttccattt aatatccctc tggagtttca ggatgaattt
     3301 ggtcatacca gtcaactagt aactgatatt cagccagttc ttgaagcaag tggtttatct
     3361 ttacattatg aagaaataac caaaggacca aattgtgtaa ttcgaggtgt tacagccaag
     3421 ggccctgtaa actcttgtca aggcaagaat tataatctga aggttactct gcctggctta
     3481 aaagaagact cacagatttt gaaaattaga ttactacctg gtcaccctcg tcgactgaaa
     3541 gtgaaacctg attctgaaat tttagttata gaaaatggaa cagctttccc atttcaggtg
     3601 gaagttttag atgaatcaga caacataaca gcacaaccaa aattgattgt tcattgtaag
     3661 ttttcaggtg ctccaaacct tccagtctat gttgtagatt gcagtagttc tggaaccagt
     3721 attttaacag gatctgcaat tcaagttcag aatattaaaa aagaccagac gcttaaagca
     3781 agaattgaaa tacctagttg taaagatgtg gcacctgtgg agaagactat taagttgctt
     3841 cccagtagcc atgttgcaag actacaaata ttcagtgtag aaggacaaaa ggcaattcag
     3901 atcaaacatc aggatgaggt taattggata gcgggtgata ttatgcataa tcttattttt
     3961 caaatgtatg atgaaggaga aagagaaatc aatataacat cagctttagc agaaaaaatt
     4021 aaagttaatt ggactcctga gattaacaaa gaacacttgc tacagggtct gcttcctgat
     4081 gtgcaagtac caacatctgt aaaagatatg cgctattgcc aggtttcatt ccaagatgat
     4141 catgtgtctt tggaaagtgc gtttacagta agaccacttc ctgatgaacc taaacattta
     4201 aaatgtgaaa tgaaaggagg aaaaacagta cagatgggcc aagagcttca aggagaagta
     4261 gttataataa ttacagatca gtacggaaat cagattcaag cattttcacc aagttcttta
     4321 tcttctttgt caattgctgg ggttggactt gatagctcaa atttgaaaac aacctttcag
     4381 gaaaacacac agagtataag tgtaagaggc atcaaattta ttccaggtcc tcctggaaat
     4441 aaggatcttt gttttacttg gcgtgagttt tctgacttta ttcgagtgca actaatttct
     4501 ggacctcctg ctaaacttct ccttatagac tggccagaac taaaggagtc cattccagtg
     4561 attaatggaa gagatttaca gaaccctatt attgttcaac tttgtgatca gtgggataat
     4621 ccagcaccgg tacaacatgt taaaataagt cttacaaaag ctagcaattt aaagctcatg
     4681 ccttcaaacc aacagcataa aacagatgag aaaggcaggg ctaatttggg agtattcagt
     4741 gtttttgccc ctaggggaga gcatactctt caggttaaag ccatctataa caaaagtatc
     4801 atagaaggac ctataattaa gttaatgatt cttccagacc cagaaaaacc cgttcgtctc
     4861 aatgttaaat atgacaaaga tgcatccttc ttagcagggg gtcttttcac tgattttatg
     4921 attagtgtta tttctgaaga tgacagtatc attaaaaaca ttaatccagc acgtatttcc
     4981 atgaaaatgt ggaagctgtc taccagtggg aaccgacccc cagcaaatgc agaaacattt
     5041 agttgtaata aaataaaaga taatgacaaa gaagatggct gcttctattt cagggataaa
     5101 gtaattccta ataaagtggg gacatattgt atccagtttg gttttatgat ggataaaaca
     5161 aatattctca acagtgaaca ggttatagtt gaagtcctgc ctaatcaacc tgtgaagtta
     5221 gtacctaaaa ttaaaccacc tacaccagct gtttcaaatg ttcgctcagt tgccagtagg
     5281 accttggtca gagatctaca tcttagtatc acggatgact acgacaacca tactggaatt
     5341 gatttggttg gcactataat agccaccatt aaaggctcta atgaggaaga tactgatacc
     5401 ccacttttta ttgggaaagt tagaacactt gaattcccct tcgtgaatgg ttcggctgaa
     5461 atcatgagtc tggtgctggc agaaagtagt cctggaaggg atagtactga atattttatt
     5521 gtatttgagc cccggctacc acttttatca agaaccttag aaccatatat cctaccgttc
     5581 atgttttaca atgatgttaa gaagcagcaa caaatggcag cacttacaaa agaaaaggac
     5641 caattatctc agtctattgt tatgtataaa agtttatttg aagccagcca acagcttctt
     5701 aatgaaatga aatgtcaagt tgaagaagca agattaaaag aggcccaatt gcgaaatgaa
     5761 ctaaaaatac ataatattga cattcctaca acacaacagg tgccacacat tgaagcactt
     5821 ctgaaaagaa agctatcaga acaagaagaa ctgaagaaaa aacctagaag atcgtgtact
     5881 cttccaaact atactaaagg cagtggagat gttttgggaa agattgcaca tctagcacaa
     5941 attgaagatg atagagctgc gatggttatt tcttggcatc tggcaagtga catggactgt
     6001 gtagtcaccc taaccactga cgctgcacgt cgtatctatg atgaaaccca aggtcgtcag
     6061 caggtgttgc cccttgattc tatttacaag aagactcttc cagattggaa aagatctcta
     6121 cctcatttcc gaaatggaaa attgtatttt aaacccattg gagatccagt ctttgctcga
     6181 gacttgttaa catttccaga taatgtagaa cattgtgaaa cagtatttgg tatgctgtta
     6241 ggagacacca ttattttgga taatctggat gcggccaatc attatagaaa agaggttgtt
     6301 aaaattacac actgtcctac actgctgacc agagatggag atcgaattcg aagtaatgga
     6361 aagtttgggg gccttcagaa taaagctcct ccaatggata aacttcgggg aatggtattt
     6421 ggagctccag ttccaaaaca gtgtctgatc ttaggggaac aaatagatct tcttcagcag
     6481 tatcgttctg ctgtgtgcaa actagacagt gtgaataagg atcttaacag tcaattagag
     6541 taccttcgca ctccggatat gaggaagaaa aagcaagaac ttgatgaaca tgagaaaaat
     6601 ctcaaactaa tagaggaaaa actaggtgct acaagacaaa ataggattat aaccaaaaca
     6661 gatgtagact acaaagacca tgacggtgat tataaagatc atgacatcga ttacaaggat
     6721 gacgatgaca agtgagtcga caatcaacct ctggattaca aaatttgtga aagattgact
     6781 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     6841 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     6901 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     6961 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     7021 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     7081 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg
     7141 acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     7201 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     7261 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     7321 gcctccccgc ctggaattct accgggtagg ggaggcgctt ttcccaaggc agtctggagc
     7381 atgcgcttta gcagccccgc tgggcacttg gcgctacaca agtggcctct ggcctcgcac
     7441 acattccaca tccaccggta ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc
     7501 accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag
     7561 gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga
     7621 gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct ccttcgcttt
     7681 ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg
     7741 cggggcgggc gcccgaaggt cctccggagg cccggcattc tgcacgcttc aaaagcgcac
     7801 gtctgccgcg ctgttctcct cttcctcatc tccgggcctt tcgacctgca gcccaagctt
     7861 accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc
     7921 gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg
     7981 gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc
     8041 gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg
     8101 gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc
     8161 ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag
     8221 gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg
     8281 ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc
     8341 ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc
     8401 gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc
     8461 tgacgcccgc cccacgaccc gcagcgcccg accgaaagga gcgcacgacc ccatgcatct
     8521 cgagggcccg gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     8581 tttaaaagaa aaggggggac tggaagggct agctcactcc caacgaagac aagatctgct
     8641 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     8701 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     8761 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     8821 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa
     8881 gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata
     8941 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     9001 tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact
     9061 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     9121 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     9181 tgaggaggct tttttggagg cctagggacg tacccaattc gccctatagt gagtcgtatt
     9241 acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
     9301 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
     9361 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta
     9421 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     9481 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     9541 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     9601 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     9661 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     9721 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     9781 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     9841 acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     9901 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     9961 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
    10021 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
    10081 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
    10141 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
    10201 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
    10261 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
    10321 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
    10381 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
    10441 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
    10501 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
    10561 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
    10621 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
    10681 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
    10741 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
    10801 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
    10861 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
    10921 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
    10981 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
    11041 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
    11101 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
    11161 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
    11221 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
    11281 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
    11341 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
    11401 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
    11461 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
    11521 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
    11581 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
    11641 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
    11701 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
    11761 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
    11821 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
    11881 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
    11941 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
    12001 aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact aaagggaaca
    12061 aaagctggag ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg
    12121 gtaacgatga gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc atgccgattg
    12181 gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc aacagacggg tctgacatgg
    12241 attggacgaa ccactgaatt gccgcattgc agagatattg tatttaagtg cctagctcga
    12301 tacataaacg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
    12361 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
    12421 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
    12481 atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct
    12541 ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg
    12601 tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg
    12661 tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg
    12721 gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg
    12781 cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac
    12841 aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc
    12901 tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag
    12961 aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccgctgat cttcagacct
    13021 ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa
    13081 attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa
    13141 agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg
    13201 ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag
    13261 cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc
    13321 tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa
    13381 cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg
    13441 aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag
    13501 tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa
    13561 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg
    13621 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga
    13681 ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag
    13741 ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc
    13801 gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac
    13861 ggatc
//
