LOCUS       sequence-300575-       13961 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses full-length human SMCHD1 with a C-terminal 3x FLAG tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13961)
  AUTHORS   Hiramuki Y, Tapscott SJ
  TITLE     Identification of SMCHD1 domains for nuclear localization, 
            homo-dimerization, and protein cleavage.
  JOURNAL   Skelet Muscle. 2018 Aug 2;8(1):24. doi: 10.1186/s13395-018-0172-z.
  PUBMED    30071896
REFERENCE   2  (bases 1 to 13961)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Skelet 
            Muscle."; date: "2018-08-2"; volume: "8(1):24. doi"; pages: " 
            10.1186/s13395-018-0172-z"
COMMENT     Sequence Label: pLenti-CMV-SMCHD1-Exon1-48-3xFLAG
FEATURES             Location/Qualifiers
     source          1..13961
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    19..135
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        158..461
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        462..665
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     616..636
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     662..686
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             6763..6828
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    6838..7426
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6891..6911)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7309..7320)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        7440..7939
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7475..7498)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     7872..7891
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             7960..8559
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7960..7979)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8456..8476
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             8697..8930
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9002..9123
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9039..9058)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9093..9112
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9163..9298
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9318..9337)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9319..9337)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9347..9364)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9347..9363)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9356..9378)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9505..9960
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9592..9611)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9802..9823
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9986..10090
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10091..10159,10160..10951)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10309..10328)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11122..11710
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11611..11630
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11864..11881
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11998..12019
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12034..12039,12040..12057,12058..12064)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12072..12088
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12077..12099
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12096..12112
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12096..12112
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12131..12151
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12133..12151
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        12179..12405
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             12406..12586
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    12633..12758
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    13251..13484
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             13669..13713
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 tcgacggtat cggttaactt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
       61 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
      121 aaaattcaaa attttatcga taagcttggg agttccgcgt tacataactt acggtaaatg
      181 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      241 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      301 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      361 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      421 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      481 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      541 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      601 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      661 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
      721 atagaagaca ccgactctag aggatccatg gcagcggcgg acggcggcgg gcctggtggg
      781 gcctctgtgg ggactgagga ggatggcgga ggcgtcggcc acaggacggt gtacttgttt
      841 gatcggcgcg aaaaggagtc cgagctcggg gaccggcctc tgcaggtcgg ggagcgctcg
      901 gactacgcgg gatttcgcgc gtgtgtgtgt cagacacttg gcatttcacc tgaagaaaaa
      961 tttgttatta caacaacaag taggaaagaa attacctgtg ataattttga tgaaactgtt
     1021 aaagatggag tcaccttata cctgctacag tcggtcaatc agttactact gacagctacg
     1081 aaagaacgaa ttgacttctt acctcactat gacacactgg ttaaaagtgg catgtatgaa
     1141 tattatgcca gtgaaggaca aaatcctttg ccatttgctc ttgcggaatt aattgacaat
     1201 tcattgtctg ctacttctcg taacattggg gttagaagaa tacagatcaa attgcttttt
     1261 gatgaaacac aaggaaaacc tgctgttgca gtgatagata atggaagagg aatgacctct
     1321 aaacagctta acaactgggc cgtgtatagg ttgtcaaaat tcacaaggca aggtgacttt
     1381 gaaagtgatc attcaggata tgttcgtcca gtaccagtgc cacgcagttt aaatagtgat
     1441 atttcctatt ttggtgttgg gggcaagcaa gctgtcttct ttgttggaca atcagccaga
     1501 atgataagca aacctgcaga ttcccaagat gttcacgagc ttgtgctttc taaagaagat
     1561 tttgagaaga aggagaaaaa taaagaggca atatatagtg gatatattag aaacagaaag
     1621 ccctctgatt ctgttcacat tacaaatgat gatgaaagat ttctacatca tcttatcata
     1681 gaggagaagg aaaaagatag ctttactgct gtggttatca caggggtaca accagaacac
     1741 atacagtact tgaaaaatta tttccacctt tggacacgac agttagcgca tatttatcac
     1801 tactatattc atggcccaaa aggaaatgaa atacgaacat caaaagaagt tgaacctttc
     1861 aacaatattg atattgaaat ttctatgttt gaaaaaggga aggtacctaa gattgtcaac
     1921 ctaagggaaa tacaagacga catgcagacg ttgtatgtaa acacagcagc tgatagtttt
     1981 gaattcaaag ctcatgttga aggagatggt gtagtggaag ggattatccg ttatcatcca
     2041 ttcttatatg atagagaaac ttaccctgat gatccatgct ttccatcaaa attaaaagat
     2101 gaagatgatg aagatgattg tttcatactt gagaaagcag ctagagggaa aaggcctatt
     2161 tttgaatgtt tttggaatgg acgattaata ccatatacat cagttgaaga ctttgactgg
     2221 tgtactcctc ctaagaagag agggcttgca ccaattgaat gctacaatag gatatctggt
     2281 gcattattca ctaatgacaa attccaggtc agcacaaata aattgacgtt tatggatctt
     2341 gagctaaaat tgaaagataa gaacaccctt tttacaagga ttttaaatgg acaggaacag
     2401 cgaatgaaaa ttgacagaga atttgctttg tggctgaagg actgtcatga gaagtatgat
     2461 aaacaaataa aatttacact ttttaaggga gtaattacac gtcctgatct tccttctaaa
     2521 aagcaaggtc cctgggcaac atatgcagca atagaatggg atggaaagat atacaaagca
     2581 ggacagctgg tcaagacaat caagacactt cccctctttt atggaagcat agtaagattt
     2641 tttctttatg gcgatcatga tggagaagta tatgctacag gaggagaggt tcaaattgca
     2701 atggaacctc aggcactata tgatgaagta agaactgtgc caattgcaaa gctggatagg
     2761 acagttgctg agaaagctgt taaaaaatat gtagaagatg aaatggcaag gctccctgat
     2821 agattgtcag taacttggcc tgaaggagat gaattattgc ctaatgaggt taggcctgct
     2881 ggaaccccta ttggtgcgtt aagaattgaa atactgaata aaaaagggga agcaatgcaa
     2941 aagcttccag gaacaagcca tggagggtca aagaaactcc tggttgagct caaagttatt
     3001 ttacattctt caagtggaaa taaagagatt atttcgcata ttagtcaaca tggaggaaaa
     3061 tggccttact ggtttaaaaa aatggaaaat attcagaagt tggggaatta taccttgaaa
     3121 ttacaagttg tgttgaatga aagtaatgca gacacttatg caggaagacc actaccatct
     3181 aaagcaatta agttttctgt taaagagggt aagccagaga aattttcatt tggtcttctg
     3241 gatcttcctt ttcgtgttgg agttccattt aatatccctc tggagtttca ggatgaattt
     3301 ggtcatacca gtcaactagt aactgatatt cagccagttc ttgaagcaag tggtttatct
     3361 ttacattatg aagaaataac caaaggacca aattgtgtaa ttcgaggtgt tacagccaag
     3421 ggccctgtaa actcttgtca aggcaagaat tataatctga aggttactct gcctggctta
     3481 aaagaagact cacagatttt gaaaattaga ttactacctg gtcaccctcg tcgactgaaa
     3541 gtgaaacctg attctgaaat tttagttata gaaaatggaa cagctttccc atttcaggtg
     3601 gaagttttag atgaatcaga caacataaca gcacaaccaa aattgattgt tcattgtaag
     3661 ttttcaggtg ctccaaacct tccagtctat gttgtagatt gcagtagttc tggaaccagt
     3721 attttaacag gatctgcaat tcaagttcag aatattaaaa aagaccagac gcttaaagca
     3781 agaattgaaa tacctagttg taaagatgtg gcacctgtgg agaagactat taagttgctt
     3841 cccagtagcc atgttgcaag actacaaata ttcagtgtag aaggacaaaa ggcaattcag
     3901 atcaaacatc aggatgaggt taattggata gcgggtgata ttatgcataa tcttattttt
     3961 caaatgtatg atgaaggaga aagagaaatc aatataacat cagctttagc agaaaaaatt
     4021 aaagttaatt ggactcctga gattaacaaa gaacacttgc tacagggtct gcttcctgat
     4081 gtgcaagtac caacatctgt aaaagatatg cgctattgcc aggtttcatt ccaagatgat
     4141 catgtgtctt tggaaagtgc gtttacagta agaccacttc ctgatgaacc taaacattta
     4201 aaatgtgaaa tgaaaggagg aaaaacagta cagatgggcc aagagcttca aggagaagta
     4261 gttataataa ttacagatca gtacggaaat cagattcaag cattttcacc aagttcttta
     4321 tcttctttgt caattgctgg ggttggactt gatagctcaa atttgaaaac aacctttcag
     4381 gaaaacacac agagtataag tgtaagaggc atcaaattta ttccaggtcc tcctggaaat
     4441 aaggatcttt gttttacttg gcgtgagttt tctgacttta ttcgagtgca actaatttct
     4501 ggacctcctg ctaaacttct ccttatagac tggccagaac taaaggagtc cattccagtg
     4561 attaatggaa gagatttaca gaaccctatt attgttcaac tttgtgatca gtgggataat
     4621 ccagcaccgg tacaacatgt taaaataagt cttacaaaag ctagcaattt aaagctcatg
     4681 ccttcaaacc aacagcataa aacagatgag aaaggcaggg ctaatttggg agtattcagt
     4741 gtttttgccc ctaggggaga gcatactctt caggttaaag ccatctataa caaaagtatc
     4801 atagaaggac ctataattaa gttaatgatt cttccagacc cagaaaaacc cgttcgtctc
     4861 aatgttaaat atgacaaaga tgcatccttc ttagcagggg gtcttttcac tgattttatg
     4921 attagtgtta tttctgaaga tgacagtatc attaaaaaca ttaatccagc acgtatttcc
     4981 atgaaaatgt ggaagctgtc taccagtggg aaccgacccc cagcaaatgc agaaacattt
     5041 agttgtaata aaataaaaga taatgacaaa gaagatggct gcttctattt cagggataaa
     5101 gtaattccta ataaagtggg gacatattgt atccagtttg gttttatgat ggataaaaca
     5161 aatattctca acagtgaaca ggttatagtt gaagtcctgc ctaatcaacc tgtgaagtta
     5221 gtacctaaaa ttaaaccacc tacaccagct gtttcaaatg ttcgctcagt tgccagtagg
     5281 accttggtca gagatctaca tcttagtatc acggatgact acgacaacca tactggaatt
     5341 gatttggttg gcactataat agccaccatt aaaggctcta atgaggaaga tactgatacc
     5401 ccacttttta ttgggaaagt tagaacactt gaattcccct tcgtgaatgg ttcggctgaa
     5461 atcatgagtc tggtgctggc agaaagtagt cctggaaggg atagtactga atattttatt
     5521 gtatttgagc cccggctacc acttttatca agaaccttag aaccatatat cctaccgttc
     5581 atgttttaca atgatgttaa gaagcagcaa caaatggcag cacttacaaa agaaaaggac
     5641 caattatctc agtctattgt tatgtataaa agtttatttg aagccagcca acagcttctt
     5701 aatgaaatga aatgtcaagt tgaagaagca agattaaaag aggcccaatt gcgaaatgaa
     5761 ctaaaaatac ataatattga cattcctaca acacaacagg tgccacacat tgaagcactt
     5821 ctgaaaagaa agctatcaga acaagaagaa ctgaagaaaa aacctagaag atcgtgtact
     5881 cttccaaact atactaaagg cagtggagat gttttgggaa agattgcaca tctagcacaa
     5941 attgaagatg atagagctgc gatggttatt tcttggcatc tggcaagtga catggactgt
     6001 gtagtcaccc taaccactga cgctgcacgt cgtatctatg atgaaaccca aggtcgtcag
     6061 caggtgttgc cccttgattc tatttacaag aagactcttc cagattggaa aagatctcta
     6121 cctcatttcc gaaatggaaa attgtatttt aaacccattg gagatccagt ctttgctcga
     6181 gacttgttaa catttccaga taatgtagaa cattgtgaaa cagtatttgg tatgctgtta
     6241 ggagacacca ttattttgga taatctggat gcggccaatc attatagaaa agaggttgtt
     6301 aaaattacac actgtcctac actgctgacc agagatggag atcgaattcg aagtaatgga
     6361 aagtttgggg gccttcagaa taaagctcct ccaatggata aacttcgggg aatggtattt
     6421 ggagctccag ttccaaaaca gtgtctgatc ttaggggaac aaatagatct tcttcagcag
     6481 tatcgttctg ctgtgtgcaa actagacagt gtgaataagg atcttaacag tcaattagag
     6541 taccttcgca ctccggatat gaggaagaaa aagcaagaac ttgatgaaca tgagaaaaat
     6601 ctcaaactaa tagaggaaaa actaggtatg actcccatac gtaagtgtaa tgactcattg
     6661 cgtcattcac caaaggttga gacgacagat tgtccagttc ctcctaaaag aatgagacga
     6721 gaagctacaa gacaaaatag gattataacc aaaacagatg tagactacaa agaccatgac
     6781 ggtgattata aagatcatga catcgattac aaggatgacg atgacaagtg agtcgacaat
     6841 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     6901 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     6961 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     7021 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     7081 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     7141 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     7201 ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc
     7261 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     7321 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     7381 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg aattctaccg
     7441 ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg
     7501 cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg
     7561 ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag
     7621 gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg
     7681 tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg
     7741 gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg
     7801 tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcctc
     7861 cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc
     7921 ctcatctccg ggcctttcga cctgcagccc aagcttacca tgaccgagta caagcccacg
     7981 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     8041 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     8101 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     8161 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     8221 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     8281 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     8341 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     8401 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     8461 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     8521 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac gcccgcccca cgacccgcag
     8581 cgcccgaccg aaaggagcgc acgaccccat gcatctcgag ggcccggtac ctttaagacc
     8641 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     8701 agggctagct cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg
     8761 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     8821 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     8881 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc
     8941 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     9001 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     9061 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     9121 ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg
     9181 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     9241 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     9301 gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt
     9361 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     9421 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     9481 tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     9541 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     9601 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     9661 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     9721 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     9781 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     9841 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     9901 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
     9961 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
    10021 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
    10081 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
    10141 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
    10201 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
    10261 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
    10321 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
    10381 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
    10441 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
    10501 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
    10561 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
    10621 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
    10681 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
    10741 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
    10801 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
    10861 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
    10921 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
    10981 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
    11041 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
    11101 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
    11161 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
    11221 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
    11281 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
    11341 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
    11401 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
    11461 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
    11521 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
    11581 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
    11641 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
    11701 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
    11761 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
    11821 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
    11881 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
    11941 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
    12001 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
    12061 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
    12121 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg
    12181 tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc
    12241 ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt
    12301 gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg
    12361 cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta
    12421 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
    12481 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
    12541 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
    12601 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
    12661 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
    12721 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
    12781 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
    12841 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
    12901 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
    12961 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
    13021 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
    13081 ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat
    13141 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
    13201 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
    13261 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
    13321 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
    13381 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
    13441 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
    13501 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
    13561 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
    13621 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
    13681 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
    13741 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
    13801 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
    13861 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
    13921 gagagagaca gagacagatc cattcgatta gtgaacggat c
//
