LOCUS       sequence-300580-        6311 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Prasinophyte anion-conducting channelrhodopsin. Codon-optimized for 
            mammalian expression (human/mouse)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6311)
  AUTHORS   Rozenberg A, Oppermann J, Wietek J, Fernandez Lahore RG, Sandaa RA, 
            Bratbak G, Hegemann P, Beja O
  TITLE     Lateral Gene Transfer of Anion-Conducting Channelrhodopsins between 
            Green Algae and Giant Viruses.
  JOURNAL   Curr Biol. 2020 Dec 21;30(24):4910-4920.e5. doi: 
            10.1016/j.cub.2020.09.056. Epub 2020 Oct 15.
  PUBMED    33065010
REFERENCE   2  (bases 1 to 6311)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2020.09.056"; journalName: "Curr Biol"; date: 
            "2020-12-21- 21"; volume: "30"; issue: "24"; pages: "4910-4920.e5"
COMMENT     Sequence Label: PymeACR1
FEATURES             Location/Qualifiers
     source          1..6311
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        119..422
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        423..626
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     577..597
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      1847..1856
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2276..2983
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(2418..2436)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2686..2705
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    3157..3278
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3194..3213)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3248..3267
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3285..3740)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3422..3443)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3634..3653
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3767..3871
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3873..4230
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3873..4064
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3896..3916)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4081..4216
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4265..5059
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4265..5059
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4319..4338)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4929..4948
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5247..5266)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5291..5338
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5667..6255
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6156..6175
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg ccatgcatta
       61 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      121 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      181 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      241 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      301 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      361 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      421 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat
      481 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      541 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac
      601 ggtgggaggt ctatataagc agagctggtt tagtgaaccg tcagatccgc tagccaccat
      661 gatgatgatg gccccccctg agtctaccga cgccgagatc gccatcagga agggcatcga
      721 gtggatcgac ttcatcatgt ctgtgagcct gttcgccttc ttttactatc agaagaccgt
      781 gaagaagaga tgcaagtttg aggtgctgta cgtgacaggc gtgggcggca tcagcgactt
      841 catcaaggtg accgcagcaa cagtggtgcc tgcaaggctg acaatccagg agaacggcat
      901 caagtttggc tggtctagat acttcggctg gctgaccaca tgcccagtgc tgctgatcca
      961 cctgagcaat ctggccggca aggaggtgtt cgatgtgagg agaatgatga agatcctggt
     1021 ggcctatcag ctgctgatgt gcagcggagc aaccgcagga atgacagaca aggataacat
     1081 caagtggggc ttctacctgt ttgccgtgtt ctggctgttt gtggtgttca ggtatggcaa
     1141 gtccatcttt gccgaggcca agatggcaat gccaaagagg gcagcccctg tgctgtacag
     1201 aatcatgggc atcttctatg gaggatgggc agcctttggc atcacctggt tcatgggacc
     1261 tgagggagtg ggatggatct ccccagacgc agcaaaggcc gtgtacgccg tgctggatat
     1321 cctgacaaag aacgtgtatg ccatgcacgg ctggtatctg cggtggtata tcctgcgcaa
     1381 gcacaataag ccagaggagt tcgtggagca ggagaacgtg gccgaggaga atcagcccaa
     1441 ggagctgaag atcctgctgg tggagcagga cgaggtgttc ggctacttct tttataacat
     1501 gctggtgcag cacggcagca aggtggatct ggccaagaac ctgagcgagc tgatggccaa
     1561 gacctccaat gtgggcatcc agtacgacat gatcatgatc aatcacgatc tggccaagca
     1621 gaacaattat cagatcatgt ttgagatccg gaagtctctg ttcatgctgc ccgtgatcag
     1681 ctacggccgg aacatcccta aggacgagat ggataatcgc accgccacag gcatcgacga
     1741 ttttctggtg gcccctttcc cagacgatga ggtgcgcaac aagatgacca agtggtccag
     1801 gagggcctct gtggacccca gcctgatcat gaacatgtcc aatatggccg ccatggccaa
     1861 ttctgccaca gcaatcccag gcaagggagc agcaccaacc atgagctttg gcacaggcgt
     1921 gaggcactcc gatgcaggac caggagtgaa caagggaggc ttcaagatgg gagcaatcgg
     1981 agaggaggca accgactgtg ataattgggt ggagcctagg cagggagcaa gacacccacc
     2041 agcaaacgac ggctccgccg atctgaattc taagctggac ctgctgatgc ggcagctgga
     2101 ggatgtgaag agctccacct ccgcccagac acgcgagatg cagcagaaga tggaccagca
     2161 gcagaaggcc ttcgagcagc agcagcagca gatgatgatg tctcagcaga tgatgcagca
     2221 gccacaacaa atgatgcagc agccacagca aatgatgcaa cagcccggac cggtggtgag
     2281 caagggcgag gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat
     2341 ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta
     2401 cgagggcacc cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg
     2461 ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga
     2521 catccccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa
     2581 cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt
     2641 catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa
     2701 gaagaccatg ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa
     2761 gggcgagatc aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa
     2821 gaccacctac aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa
     2881 gttggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga
     2941 gggccgccac tccaccggcg gcatggacga gctgtacaag taagagctca agcttcgaat
     3001 tctgcagtcg acggtaccgc gggcccggga tccaccggat ctagataact gatcataatc
     3061 agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg
     3121 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat
     3181 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     3241 tctagttgtg gtttgtccaa actcatcaat gtatcttaac gcgtaaattg taagcgttaa
     3301 tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc
     3361 cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt
     3421 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa
     3481 aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg
     3541 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg
     3601 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc
     3661 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa
     3721 tgcgccgcta cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     3781 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     3841 aatgcttcaa taatattgaa aaaggaagag tcctgaggcg gaaagaacca gctgtggaat
     3901 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     3961 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     4021 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     4081 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     4141 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     4201 ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa gagacaggat gaggatcgtt
     4261 tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct
     4321 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct
     4381 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga
     4441 actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc
     4501 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg
     4561 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc
     4621 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca
     4681 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga
     4741 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgagcatgcc
     4801 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga
     4861 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca
     4921 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg
     4981 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct
     5041 tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc
     5101 aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
     5161 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
     5221 ttcgcccacc ctagggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc
     5281 cgcgctatga cggcaataaa aagacagaat aaaacgcacg gtgttgggtc gtttgttcat
     5341 aaacgcgggg ttcggtccca gggctggcac tctgtcgata ccccaccgag accccattgg
     5401 ggccaatacg cccgcgtttc ttccttttcc ccaccccacc ccccaagttc gggtgaaggc
     5461 ccagggctcg cagccaacgt cggggcggca ggccctgcca tagcctcagg ttactcatat
     5521 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     5581 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     5641 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     5701 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     5761 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     5821 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     5881 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     5941 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     6001 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     6061 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     6121 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     6181 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     6241 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     6301 ccttttgctc a
//
