LOCUS       sequence-300581-       13949 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human SMCHD1 deltaKRMR with a C-terminal 3xFLAG tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13949)
  AUTHORS   Hiramuki Y, Tapscott SJ
  TITLE     Identification of SMCHD1 domains for nuclear localization, 
            homo-dimerization, and protein cleavage.
  JOURNAL   Skelet Muscle. 2018 Aug 2;8(1):24. doi: 10.1186/s13395-018-0172-z.
  PUBMED    30071896
REFERENCE   2  (bases 1 to 13949)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Skelet 
            Muscle."; date: "2018-08-2"; volume: "8(1):24. doi"; pages: " 
            10.1186/s13395-018-0172-z"
COMMENT     Sequence Label: pLenti-CMV-SMCHD1-deltaKRMR-3xFLAG
FEATURES             Location/Qualifiers
     source          1..13949
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    19..135
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        158..461
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        462..665
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     616..636
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     662..686
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             6751..6816
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    6826..7414
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6879..6899)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7297..7308)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        7428..7927
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7463..7486)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     7860..7879
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             7948..8547
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7948..7967)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8444..8464
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             8685..8918
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8990..9111
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9027..9046)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9081..9100
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9151..9286
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9306..9325)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9307..9325)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9335..9352)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9335..9351)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9344..9366)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9493..9948
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9580..9599)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9790..9811
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9974..10078
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10079..10147,10148..10939)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10297..10316)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11110..11698
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11599..11618
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11852..11869
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11986..12007
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12022..12027,12028..12045,12046..12052)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12060..12076
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12065..12087
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12084..12100
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12084..12100
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12119..12139
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12121..12139
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        12167..12393
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             12394..12574
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    12621..12746
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    13239..13472
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             13657..13701
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 tcgacggtat cggttaactt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
       61 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
      121 aaaattcaaa attttatcga taagcttggg agttccgcgt tacataactt acggtaaatg
      181 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      241 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      301 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      361 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      421 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      481 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      541 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      601 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      661 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
      721 atagaagaca ccgactctag aggatccatg gcagcggcgg acggcggcgg gcctggtggg
      781 gcctctgtgg ggactgagga ggatggcgga ggcgtcggcc acaggacggt gtacttgttt
      841 gatcggcgcg aaaaggagtc cgagctcggg gaccggcctc tgcaggtcgg ggagcgctcg
      901 gactacgcgg gatttcgcgc gtgtgtgtgt cagacacttg gcatttcacc tgaagaaaaa
      961 tttgttatta caacaacaag taggaaagaa attacctgtg ataattttga tgaaactgtt
     1021 aaagatggag tcaccttata cctgctacag tcggtcaatc agttactact gacagctacg
     1081 aaagaacgaa ttgacttctt acctcactat gacacactgg ttaaaagtgg catgtatgaa
     1141 tattatgcca gtgaaggaca aaatcctttg ccatttgctc ttgcggaatt aattgacaat
     1201 tcattgtctg ctacttctcg taacattggg gttagaagaa tacagatcaa attgcttttt
     1261 gatgaaacac aaggaaaacc tgctgttgca gtgatagata atggaagagg aatgacctct
     1321 aaacagctta acaactgggc cgtgtatagg ttgtcaaaat tcacaaggca aggtgacttt
     1381 gaaagtgatc attcaggata tgttcgtcca gtaccagtgc cacgcagttt aaatagtgat
     1441 atttcctatt ttggtgttgg gggcaagcaa gctgtcttct ttgttggaca atcagccaga
     1501 atgataagca aacctgcaga ttcccaagat gttcacgagc ttgtgctttc taaagaagat
     1561 tttgagaaga aggagaaaaa taaagaggca atatatagtg gatatattag aaacagaaag
     1621 ccctctgatt ctgttcacat tacaaatgat gatgaaagat ttctacatca tcttatcata
     1681 gaggagaagg aaaaagatag ctttactgct gtggttatca caggggtaca accagaacac
     1741 atacagtact tgaaaaatta tttccacctt tggacacgac agttagcgca tatttatcac
     1801 tactatattc atggcccaaa aggaaatgaa atacgaacat caaaagaagt tgaacctttc
     1861 aacaatattg atattgaaat ttctatgttt gaaaaaggga aggtacctaa gattgtcaac
     1921 ctaagggaaa tacaagacga catgcagacg ttgtatgtaa acacagcagc tgatagtttt
     1981 gaattcaaag ctcatgttga aggagatggt gtagtggaag ggattatccg ttatcatcca
     2041 ttcttatatg atagagaaac ttaccctgat gatccatgct ttccatcaaa attaaaagat
     2101 gaagatgatg aagatgattg tttcatactt gagaaagcag ctagagggaa aaggcctatt
     2161 tttgaatgtt tttggaatgg acgattaata ccatatacat cagttgaaga ctttgactgg
     2221 tgtactcctc ctaagaagag agggcttgca ccaattgaat gctacaatag gatatctggt
     2281 gcattattca ctaatgacaa attccaggtc agcacaaata aattgacgtt tatggatctt
     2341 gagctaaaat tgaaagataa gaacaccctt tttacaagga ttttaaatgg acaggaacag
     2401 cgaatgaaaa ttgacagaga atttgctttg tggctgaagg actgtcatga gaagtatgat
     2461 aaacaaataa aatttacact ttttaaggga gtaattacac gtcctgatct tccttctaaa
     2521 aagcaaggtc cctgggcaac atatgcagca atagaatggg atggaaagat atacaaagca
     2581 ggacagctgg tcaagacaat caagacactt cccctctttt atggaagcat agtaagattt
     2641 tttctttatg gcgatcatga tggagaagta tatgctacag gaggagaggt tcaaattgca
     2701 atggaacctc aggcactata tgatgaagta agaactgtgc caattgcaaa gctggatagg
     2761 acagttgctg agaaagctgt taaaaaatat gtagaagatg aaatggcaag gctccctgat
     2821 agattgtcag taacttggcc tgaaggagat gaattattgc ctaatgaggt taggcctgct
     2881 ggaaccccta ttggtgcgtt aagaattgaa atactgaata aaaaagggga agcaatgcaa
     2941 aagcttccag gaacaagcca tggagggtca aagaaactcc tggttgagct caaagttatt
     3001 ttacattctt caagtggaaa taaagagatt atttcgcata ttagtcaaca tggaggaaaa
     3061 tggccttact ggtttaaaaa aatggaaaat attcagaagt tggggaatta taccttgaaa
     3121 ttacaagttg tgttgaatga aagtaatgca gacacttatg caggaagacc actaccatct
     3181 aaagcaatta agttttctgt taaagagggt aagccagaga aattttcatt tggtcttctg
     3241 gatcttcctt ttcgtgttgg agttccattt aatatccctc tggagtttca ggatgaattt
     3301 ggtcatacca gtcaactagt aactgatatt cagccagttc ttgaagcaag tggtttatct
     3361 ttacattatg aagaaataac caaaggacca aattgtgtaa ttcgaggtgt tacagccaag
     3421 ggccctgtaa actcttgtca aggcaagaat tataatctga aggttactct gcctggctta
     3481 aaagaagact cacagatttt gaaaattaga ttactacctg gtcaccctcg tcgactgaaa
     3541 gtgaaacctg attctgaaat tttagttata gaaaatggaa cagctttccc atttcaggtg
     3601 gaagttttag atgaatcaga caacataaca gcacaaccaa aattgattgt tcattgtaag
     3661 ttttcaggtg ctccaaacct tccagtctat gttgtagatt gcagtagttc tggaaccagt
     3721 attttaacag gatctgcaat tcaagttcag aatattaaaa aagaccagac gcttaaagca
     3781 agaattgaaa tacctagttg taaagatgtg gcacctgtgg agaagactat taagttgctt
     3841 cccagtagcc atgttgcaag actacaaata ttcagtgtag aaggacaaaa ggcaattcag
     3901 atcaaacatc aggatgaggt taattggata gcgggtgata ttatgcataa tcttattttt
     3961 caaatgtatg atgaaggaga aagagaaatc aatataacat cagctttagc agaaaaaatt
     4021 aaagttaatt ggactcctga gattaacaaa gaacacttgc tacagggtct gcttcctgat
     4081 gtgcaagtac caacatctgt aaaagatatg cgctattgcc aggtttcatt ccaagatgat
     4141 catgtgtctt tggaaagtgc gtttacagta agaccacttc ctgatgaacc taaacattta
     4201 aaatgtgaaa tgaaaggagg aaaaacagta cagatgggcc aagagcttca aggagaagta
     4261 gttataataa ttacagatca gtacggaaat cagattcaag cattttcacc aagttcttta
     4321 tcttctttgt caattgctgg ggttggactt gatagctcaa atttgaaaac aacctttcag
     4381 gaaaacacac agagtataag tgtaagaggc atcaaattta ttccaggtcc tcctggaaat
     4441 aaggatcttt gttttacttg gcgtgagttt tctgacttta ttcgagtgca actaatttct
     4501 ggacctcctg ctaaacttct ccttatagac tggccagaac taaaggagtc cattccagtg
     4561 attaatggaa gagatttaca gaaccctatt attgttcaac tttgtgatca gtgggataat
     4621 ccagcaccgg tacaacatgt taaaataagt cttacaaaag ctagcaattt aaagctcatg
     4681 ccttcaaacc aacagcataa aacagatgag aaaggcaggg ctaatttggg agtattcagt
     4741 gtttttgccc ctaggggaga gcatactctt caggttaaag ccatctataa caaaagtatc
     4801 atagaaggac ctataattaa gttaatgatt cttccagacc cagaaaaacc cgttcgtctc
     4861 aatgttaaat atgacaaaga tgcatccttc ttagcagggg gtcttttcac tgattttatg
     4921 attagtgtta tttctgaaga tgacagtatc attaaaaaca ttaatccagc acgtatttcc
     4981 atgaaaatgt ggaagctgtc taccagtggg aaccgacccc cagcaaatgc agaaacattt
     5041 agttgtaata aaataaaaga taatgacaaa gaagatggct gcttctattt cagggataaa
     5101 gtaattccta ataaagtggg gacatattgt atccagtttg gttttatgat ggataaaaca
     5161 aatattctca acagtgaaca ggttatagtt gaagtcctgc ctaatcaacc tgtgaagtta
     5221 gtacctaaaa ttaaaccacc tacaccagct gtttcaaatg ttcgctcagt tgccagtagg
     5281 accttggtca gagatctaca tcttagtatc acggatgact acgacaacca tactggaatt
     5341 gatttggttg gcactataat agccaccatt aaaggctcta atgaggaaga tactgatacc
     5401 ccacttttta ttgggaaagt tagaacactt gaattcccct tcgtgaatgg ttcggctgaa
     5461 atcatgagtc tggtgctggc agaaagtagt cctggaaggg atagtactga atattttatt
     5521 gtatttgagc cccggctacc acttttatca agaaccttag aaccatatat cctaccgttc
     5581 atgttttaca atgatgttaa gaagcagcaa caaatggcag cacttacaaa agaaaaggac
     5641 caattatctc agtctattgt tatgtataaa agtttatttg aagccagcca acagcttctt
     5701 aatgaaatga aatgtcaagt tgaagaagca agattaaaag aggcccaatt gcgaaatgaa
     5761 ctaaaaatac ataatattga cattcctaca acacaacagg tgccacacat tgaagcactt
     5821 ctgaaaagaa agctatcaga acaagaagaa ctgaagaaaa aacctagaag atcgtgtact
     5881 cttccaaact atactaaagg cagtggagat gttttgggaa agattgcaca tctagcacaa
     5941 attgaagatg atagagctgc gatggttatt tcttggcatc tggcaagtga catggactgt
     6001 gtagtcaccc taaccactga cgctgcacgt cgtatctatg atgaaaccca aggtcgtcag
     6061 caggtgttgc cccttgattc tatttacaag aagactcttc cagattggaa aagatctcta
     6121 cctcatttcc gaaatggaaa attgtatttt aaacccattg gagatccagt ctttgctcga
     6181 gacttgttaa catttccaga taatgtagaa cattgtgaaa cagtatttgg tatgctgtta
     6241 ggagacacca ttattttgga taatctggat gcggccaatc attatagaaa agaggttgtt
     6301 aaaattacac actgtcctac actgctgacc agagatggag atcgaattcg aagtaatgga
     6361 aagtttgggg gccttcagaa taaagctcct ccaatggata aacttcgggg aatggtattt
     6421 ggagctccag ttccaaaaca gtgtctgatc ttaggggaac aaatagatct tcttcagcag
     6481 tatcgttctg ctgtgtgcaa actagacagt gtgaataagg atcttaacag tcaattagag
     6541 taccttcgca ctccggatat gaggaagaaa aagcaagaac ttgatgaaca tgagaaaaat
     6601 ctcaaactaa tagaggaaaa actaggtatg actcccatac gtaagtgtaa tgactcattg
     6661 cgtcattcac caaaggttga gacgacagat tgtccagttc ctcctcgaga agctacaaga
     6721 caaaatagga ttataaccaa aacagatgta gactacaaag accatgacgg tgattataaa
     6781 gatcatgaca tcgattacaa ggatgacgat gacaagtgag tcgacaatca acctctggat
     6841 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     6901 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     6961 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     7021 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     7081 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     7141 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     7201 tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc
     7261 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     7321 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     7381 acgagtcgga tctccctttg ggccgcctcc ccgcctggaa ttctaccggg taggggaggc
     7441 gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta
     7501 cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc
     7561 gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc
     7621 cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct
     7681 cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca
     7741 atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg
     7801 cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc
     7861 attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg
     7921 cctttcgacc tgcagcccaa gcttaccatg accgagtaca agcccacggt gcgcctcgcc
     7981 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc
     8041 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa
     8101 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc
     8161 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc
     8221 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc
     8281 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg
     8341 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc
     8401 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac
     8461 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg
     8521 tgcatgaccc gcaagcccgg tgcctgacgc ccgccccacg acccgcagcg cccgaccgaa
     8581 aggagcgcac gaccccatgc atctcgaggg cccggtacct ttaagaccaa tgacttacaa
     8641 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctagctca
     8701 ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     8761 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     8821 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     8881 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat
     8941 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat
     9001 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     9061 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     9121 gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     9181 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     9241 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca
     9301 attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg
     9361 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     9421 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     9481 atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     9541 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     9601 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     9661 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     9721 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     9781 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     9841 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     9901 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt
     9961 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
    10021 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
    10081 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
    10141 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
    10201 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
    10261 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
    10321 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
    10381 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
    10441 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
    10501 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
    10561 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
    10621 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
    10681 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
    10741 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
    10801 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
    10861 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
    10921 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
    10981 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
    11041 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
    11101 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
    11161 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
    11221 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
    11281 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
    11341 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
    11401 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
    11461 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
    11521 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
    11581 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
    11641 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
    11701 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
    11761 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
    11821 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
    11881 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
    11941 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
    12001 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
    12061 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc
    12121 aattaaccct cactaaaggg aacaaaagct ggagctgcaa gcttaatgta gtcttatgca
    12181 atactcttgt agtcttgcaa catggtaacg atgagttagc aacatgcctt acaaggagag
    12241 aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc cttattagga
    12301 aggcaacaga cgggtctgac atggattgga cgaaccactg aattgccgca ttgcagagat
    12361 attgtattta agtgcctagc tcgatacata aacgggtctc tctggttaga ccagatctga
    12421 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
    12481 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
    12541 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
    12601 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
    12661 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
    12721 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
    12781 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
    12841 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
    12901 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
    12961 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
    13021 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
    13081 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
    13141 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
    13201 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
    13261 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
    13321 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
    13381 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
    13441 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
    13501 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
    13561 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
    13621 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
    13681 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
    13741 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
    13801 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
    13861 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
    13921 gacagatcca ttcgattagt gaacggatc
//
