LOCUS       sequence-300588-       12647 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human SMCHD1 Exon1-36.47M-48 with a C-terminal 3xFLAG tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12647)
  AUTHORS   Hiramuki Y, Tapscott SJ
  TITLE     Identification of SMCHD1 domains for nuclear localization, 
            homo-dimerization, and protein cleavage.
  JOURNAL   Skelet Muscle. 2018 Aug 2;8(1):24. doi: 10.1186/s13395-018-0172-z.
  PUBMED    30071896
REFERENCE   2  (bases 1 to 12647)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Skelet 
            Muscle."; date: "2018-08-2"; volume: "8(1):24. doi"; pages: " 
            10.1186/s13395-018-0172-z"
COMMENT     Sequence Label: pLenti-CMV-SMCHD1-Exon1-36.47M-48-3xFLAG
FEATURES             Location/Qualifiers
     source          1..12647
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    19..135
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        158..461
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        462..665
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     616..636
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     662..686
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             5449..5514
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    5524..6112
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5577..5597)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5995..6006)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        6126..6625
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6161..6184)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6558..6577
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6646..7245
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6646..6665)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7142..7162
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             7383..7616
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7688..7809
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7725..7744)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7779..7798
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7849..7984
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8004..8023)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8005..8023)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8033..8050)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8033..8049)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8042..8064)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8191..8646
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8278..8297)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8488..8509
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8672..8776
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8777..8845,8846..9637)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8995..9014)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9808..10396
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10297..10316
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10550..10567
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10684..10705
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10720..10725,10726..10743,10744..10750)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10758..10774
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10763..10785
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10782..10798
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10782..10798
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10817..10837
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10819..10837
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        10865..11091
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             11092..11272
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    11319..11444
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11937..12170
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             12355..12399
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 tcgacggtat cggttaactt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
       61 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
      121 aaaattcaaa attttatcga taagcttggg agttccgcgt tacataactt acggtaaatg
      181 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      241 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      301 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      361 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      421 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      481 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      541 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      601 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      661 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
      721 atagaagaca ccgactctag aggatccatg gcagcggcgg acggcggcgg gcctggtggg
      781 gcctctgtgg ggactgagga ggatggcgga ggcgtcggcc acaggacggt gtacttgttt
      841 gatcggcgcg aaaaggagtc cgagctcggg gaccggcctc tgcaggtcgg ggagcgctcg
      901 gactacgcgg gatttcgcgc gtgtgtgtgt cagacacttg gcatttcacc tgaagaaaaa
      961 tttgttatta caacaacaag taggaaagaa attacctgtg ataattttga tgaaactgtt
     1021 aaagatggag tcaccttata cctgctacag tcggtcaatc agttactact gacagctacg
     1081 aaagaacgaa ttgacttctt acctcactat gacacactgg ttaaaagtgg catgtatgaa
     1141 tattatgcca gtgaaggaca aaatcctttg ccatttgctc ttgcggaatt aattgacaat
     1201 tcattgtctg ctacttctcg taacattggg gttagaagaa tacagatcaa attgcttttt
     1261 gatgaaacac aaggaaaacc tgctgttgca gtgatagata atggaagagg aatgacctct
     1321 aaacagctta acaactgggc cgtgtatagg ttgtcaaaat tcacaaggca aggtgacttt
     1381 gaaagtgatc attcaggata tgttcgtcca gtaccagtgc cacgcagttt aaatagtgat
     1441 atttcctatt ttggtgttgg gggcaagcaa gctgtcttct ttgttggaca atcagccaga
     1501 atgataagca aacctgcaga ttcccaagat gttcacgagc ttgtgctttc taaagaagat
     1561 tttgagaaga aggagaaaaa taaagaggca atatatagtg gatatattag aaacagaaag
     1621 ccctctgatt ctgttcacat tacaaatgat gatgaaagat ttctacatca tcttatcata
     1681 gaggagaagg aaaaagatag ctttactgct gtggttatca caggggtaca accagaacac
     1741 atacagtact tgaaaaatta tttccacctt tggacacgac agttagcgca tatttatcac
     1801 tactatattc atggcccaaa aggaaatgaa atacgaacat caaaagaagt tgaacctttc
     1861 aacaatattg atattgaaat ttctatgttt gaaaaaggga aggtacctaa gattgtcaac
     1921 ctaagggaaa tacaagacga catgcagacg ttgtatgtaa acacagcagc tgatagtttt
     1981 gaattcaaag ctcatgttga aggagatggt gtagtggaag ggattatccg ttatcatcca
     2041 ttcttatatg atagagaaac ttaccctgat gatccatgct ttccatcaaa attaaaagat
     2101 gaagatgatg aagatgattg tttcatactt gagaaagcag ctagagggaa aaggcctatt
     2161 tttgaatgtt tttggaatgg acgattaata ccatatacat cagttgaaga ctttgactgg
     2221 tgtactcctc ctaagaagag agggcttgca ccaattgaat gctacaatag gatatctggt
     2281 gcattattca ctaatgacaa attccaggtc agcacaaata aattgacgtt tatggatctt
     2341 gagctaaaat tgaaagataa gaacaccctt tttacaagga ttttaaatgg acaggaacag
     2401 cgaatgaaaa ttgacagaga atttgctttg tggctgaagg actgtcatga gaagtatgat
     2461 aaacaaataa aatttacact ttttaaggga gtaattacac gtcctgatct tccttctaaa
     2521 aagcaaggtc cctgggcaac atatgcagca atagaatggg atggaaagat atacaaagca
     2581 ggacagctgg tcaagacaat caagacactt cccctctttt atggaagcat agtaagattt
     2641 tttctttatg gcgatcatga tggagaagta tatgctacag gaggagaggt tcaaattgca
     2701 atggaacctc aggcactata tgatgaagta agaactgtgc caattgcaaa gctggatagg
     2761 acagttgctg agaaagctgt taaaaaatat gtagaagatg aaatggcaag gctccctgat
     2821 agattgtcag taacttggcc tgaaggagat gaattattgc ctaatgaggt taggcctgct
     2881 ggaaccccta ttggtgcgtt aagaattgaa atactgaata aaaaagggga agcaatgcaa
     2941 aagcttccag gaacaagcca tggagggtca aagaaactcc tggttgagct caaagttatt
     3001 ttacattctt caagtggaaa taaagagatt atttcgcata ttagtcaaca tggaggaaaa
     3061 tggccttact ggtttaaaaa aatggaaaat attcagaagt tggggaatta taccttgaaa
     3121 ttacaagttg tgttgaatga aagtaatgca gacacttatg caggaagacc actaccatct
     3181 aaagcaatta agttttctgt taaagagggt aagccagaga aattttcatt tggtcttctg
     3241 gatcttcctt ttcgtgttgg agttccattt aatatccctc tggagtttca ggatgaattt
     3301 ggtcatacca gtcaactagt aactgatatt cagccagttc ttgaagcaag tggtttatct
     3361 ttacattatg aagaaataac caaaggacca aattgtgtaa ttcgaggtgt tacagccaag
     3421 ggccctgtaa actcttgtca aggcaagaat tataatctga aggttactct gcctggctta
     3481 aaagaagact cacagatttt gaaaattaga ttactacctg gtcaccctcg tcgactgaaa
     3541 gtgaaacctg attctgaaat tttagttata gaaaatggaa cagctttccc atttcaggtg
     3601 gaagttttag atgaatcaga caacataaca gcacaaccaa aattgattgt tcattgtaag
     3661 ttttcaggtg ctccaaacct tccagtctat gttgtagatt gcagtagttc tggaaccagt
     3721 attttaacag gatctgcaat tcaagttcag aatattaaaa aagaccagac gcttaaagca
     3781 agaattgaaa tacctagttg taaagatgtg gcacctgtgg agaagactat taagttgctt
     3841 cccagtagcc atgttgcaag actacaaata ttcagtgtag aaggacaaaa ggcaattcag
     3901 atcaaacatc aggatgaggt taattggata gcgggtgata ttatgcataa tcttattttt
     3961 caaatgtatg atgaaggaga aagagaaatc aatataacat cagctttagc agaaaaaatt
     4021 aaagttaatt ggactcctga gattaacaaa gaacacttgc tacagggtct gcttcctgat
     4081 gtgcaagtac caacatctgt aaaagatatg cgctattgcc aggtttcatt ccaagatgat
     4141 catgtgtctt tggaaagtgc gtttacagta agaccacttc ctgatgaacc taaacattta
     4201 aaatgtgaaa tgaaaggagg aaaaacagta cagatgggcc aagagcttca aggagaagta
     4261 gttataataa ttacagatca gtacggaaat cagattcaag cattttcacc aagttcttta
     4321 tcttctttgt caattgctgg ggttggactt gatagctcaa atttgaaaac aacctttcag
     4381 gaaaacacac agagtataag tgtaagaggc atcaaattta ttccaggtcc tcctggaaat
     4441 aaggatcttt gttttacttg gcgtgagttt tctgacttta ttcgagtgca actaatttct
     4501 ggacctcctg ctaaacttct ccttatagac tggccagaac taaaggagtc cattccagtg
     4561 attaatggaa gagatttaca gaaccctatt attgttcaac tttgtgatca gtgggataat
     4621 ccagcaccgg tacaacatgt taaaataagt cttacaaaag ctagcaattt aaagctcatg
     4681 ccttcaaacc aacagcataa aacagatgag aaaggcaggg ctaatttggg agtattcagt
     4741 gtttttgccc ctaggggaga gcatactctt caggttaaag ccatctataa caaaagtatc
     4801 atagaaggac ctataattaa gttaatgatt cttccagacc cagaaaaacc cgttcgtctc
     4861 aatgttaaat atgacaaaga tgcatccttc ttagcagggg gtcttttcac tgattttatg
     4921 attagtgtta tttctgaaga tgacagtatc attaaaaaca ttaatccagc acgtatttcc
     4981 atgaaaatgt ggaagctgtc taccagtggg aaccgacccc cagcaaatgc agaaacattt
     5041 agttgtaata aaataaaaga taatgacaaa gaagatggct gcttctattt cagggataaa
     5101 gtaattccta ataaagtggg gacatattgt atccagtttg gttttatgat ggataaaaca
     5161 aatattctca acagtgaaca ggttatagtt gaagtcctgc ctaatcaacc tgtgaagtta
     5221 gtacctaaaa ttaaaccacc tacaccagct gtttcaaatg ttcgctcagt tgccagtagg
     5281 accttggtca gagatctaca tcttagtatc acgatgactc ccatacgtaa gtgtaatgac
     5341 tcattgcgtc attcaccaaa ggttgagacg acagattgtc cagttcctcc taaaagaatg
     5401 agacgagaag ctacaagaca aaataggatt ataaccaaaa cagatgtaga ctacaaagac
     5461 catgacggtg attataaaga tcatgacatc gattacaagg atgacgatga caagtgagtc
     5521 gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     5581 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     5641 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     5701 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     5761 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     5821 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     5881 ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg
     5941 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     6001 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     6061 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggaatt
     6121 ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc
     6181 gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg
     6241 taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct
     6301 agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt
     6361 agcacgtctc actagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg
     6421 cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg
     6481 aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag
     6541 gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc
     6601 ctcttcctca tctccgggcc tttcgacctg cagcccaagc ttaccatgac cgagtacaag
     6661 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc
     6721 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg
     6781 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
     6841 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg
     6901 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
     6961 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
     7021 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
     7081 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc
     7141 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
     7201 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgacgccc gccccacgac
     7261 ccgcagcgcc cgaccgaaag gagcgcacga ccccatgcat ctcgagggcc cggtaccttt
     7321 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     7381 actggaaggg ctagctcact cccaacgaag acaagatctg ctttttgctt gtactgggtc
     7441 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     7501 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     7561 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag
     7621 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag
     7681 tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     7741 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     7801 tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta
     7861 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     7921 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     7981 ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg
     8041 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
     8101 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     8161 aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg
     8221 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     8281 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     8341 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     8401 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     8461 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     8521 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     8581 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
     8641 caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     8701 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     8761 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     8821 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     8881 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     8941 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     9001 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     9061 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     9121 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     9181 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     9241 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     9301 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     9361 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     9421 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     9481 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     9541 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     9601 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     9661 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     9721 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     9781 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     9841 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     9901 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     9961 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
    10021 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
    10081 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
    10141 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
    10201 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
    10261 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
    10321 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
    10381 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
    10441 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
    10501 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
    10561 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
    10621 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
    10681 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
    10741 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca
    10801 tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc
    10861 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
    10921 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
    10981 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
    11041 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
    11101 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
    11161 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
    11221 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
    11281 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
    11341 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
    11401 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
    11461 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
    11521 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
    11581 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
    11641 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
    11701 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
    11761 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
    11821 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
    11881 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
    11941 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
    12001 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
    12061 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
    12121 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
    12181 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
    12241 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
    12301 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
    12361 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
    12421 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
    12481 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
    12541 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
    12601 ggtggagaga gagacagaga cagatccatt cgattagtga acggatc
//
