LOCUS       sequence-300597-        4350 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for SARS-CoV-2 ORF10 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4350)
  TITLE     SARS-CoV-2 protein Mammalian expression constructs, tagged, for 
            protein expression and/or purification
REFERENCE   2  (bases 1 to 4350)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4134487
FEATURES             Location/Qualifiers
     source          1..4350
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    62..183
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(99..118)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     153..172
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(236..262)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(263..283)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     regulatory      394..403
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(472..674)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(501..521)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        676..979
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     polyA_signal    1060..1108
                     /note="synthetic polyadenylation signal"
     terminator      1126..1172
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     misc_feature    1177..1248
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(1400..1536)
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     CDS             complement(join(1549..2340,2341..2409))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2172..2191
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3155..3743
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3644..3663
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    3969..4040
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(4085..4221)
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     terminator      complement(4251..4297)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
       61 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
      121 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
      181 ttatcatgtc tgtgagctga aggtacgctg tatctcagtc agtcaagcta gcttacgcat
      241 aatctggtac gtcatagggg tacgactgaa agtaaaggtt ttcagttagg ttaaagttca
      301 cgacgtctac ctgagcaatg taattgcgag agttcatcct acataaaagg agtgaataga
      361 tcgtgaatgg aaaggcgaaa acatttatgt accccatggt ggcggccgct ggcgggtgtg
      421 tcgagtggat ggtaggatcg acaaagatct gatctgacgg ttcactaaac cagctctgct
      481 tatatagacc tcccaccgta cacgcctacc gcccatttgc gtcaatgggg cggagttgtt
      541 acgacatttt ggaaagtccc gttgattttg gtgccaaaac aaactcccat tgacgtcaat
      601 ggggtggaga cttggaaatc cccgtgagtc aaaccgctat ccacgcccat tgatgtactg
      661 ccaaaaccgc atcagcatgg taatagcgat gactaatacg tagatgtact gccaagtagg
      721 aaagtcccat aaggtcatgt actgggcata atgccaggcg ggccatttac cgtcattgac
      781 gtcaataggg ggcgtacttg gcatatgata cacttgatgt actgccaagt gggcagttta
      841 ccgtaaatac tccacccatt gacgtcaatg gaaagtccct attggcgtta ctatgggaac
      901 atacgtcatt attgacgtca atgggcgggg gtcgttgggc ggtcagccag gcgggccatt
      961 taccgtaagt tatgtaacgc ggaactccat atatgggcta tgaactaatg accccgtaat
     1021 tgattactag atctggctac actcgatcag cagttagata ataaaatcgc tatccatcga
     1081 agatggatgt gtgttggttt tttgtgtgtg taacgcaacg attgatagca taaccccttg
     1141 gggcctctaa acgggtcttg aggggttttt tgaattgaga ggtagctgaa gctgctgtta
     1201 tgaccactag agggaagaag atacctgtgg agctaatggt ccaagatgtc gggagccgcg
     1261 atcgctgcca aaaaggtaag ctgacgatag ctgacgagat tcgatgcccg acgagcaaat
     1321 gagcgccacg gctgttcgcg taagctcgaa acgacggaga ttcgatagag ccagagtcga
     1381 gtttaaacga ttcgggatct taaggataaa tttctggtaa ggaggacacg tatggaagtg
     1441 ggcaagttgg ggaagccgta tccgttgctg aatctggcat atgtgggagt ataagacgcg
     1501 cagcgtcgca tcaggcattt ttttctgcgc caatgcacct agaatcggtt accaatgctt
     1561 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     1621 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     1681 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     1741 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctatcaactg
     1801 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttacgcaagg ttgttgccat
     1861 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     1921 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     1981 cggtcctcca atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     2041 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     2101 atactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     2161 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     2221 gcgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     2281 acccactcgc gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     2341 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     2401 aatactcatg cgaaagcctc ctgctgatcc tgtctcttga tagatgtttg caaaagctag
     2461 gactcgatta tacgttgacc gatcagacta tcaatatcta ggtttaaaca gatcggtacg
     2521 ctgccgtgac caggcttgag tacaatatct gacgattgga cttcctgggt cccaagctgg
     2581 attccgactg gctgactgcc aagcaacgat atggatcgtc gagactcgca ctgagaggat
     2641 cgttctcggt acaccaggcg cgccccgatt tcgatattcc tcggatctcg gatagatcgg
     2701 gattgaggat gtaggttgaa cttctctcgg gtgatcccga tctcggatat tcgggattgg
     2761 agtaatacct ggcgataggc gctgccaagc aatagccaag gcgcaaggcc ggccaagaga
     2821 cttgcgatag ttcgttcgag aggtagcctg caagatggat cggacgaaag gtttgaagat
     2881 tttccgacga gggccttgga gagttcggaa atttaaatgc aactatggat gaacgaaata
     2941 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     3001 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     3061 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     3121 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     3181 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     3241 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     3301 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     3361 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     3421 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     3481 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     3541 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     3601 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     3661 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     3721 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     3781 tttgctggcc ttttgctcac atgatttaaa tgaaactcgt aatcggagaa cgggtgcaat
     3841 agcaaggacc agatcgaact tgactgacgc aattctgagc gtagtcgaaa cccagacaag
     3901 tcgatctcgc caagtagctg acttgactgg ttaattaagt ttcgatagcc caaggtaacc
     3961 aatgagatta gggagggaag aaagtttgat gaactacttc tgacccctag tggtgtccag
     4021 aaaagaccat taaaggaatg tcttgatcga aatcgagaga tcgcctgcag gatagctgac
     4081 gaccttaagg ataaatttct ggtaaggagg acacgtatgg aagtgggcaa gttggggaag
     4141 ccgtatccgt tgctgaatct ggcatatgtg ggagtataag acgcgcagcg tcgcatcagg
     4201 catttttttc tgcgccaatg ccggtagata gcaatccggc ccgaggggca caaaaaaccc
     4261 ctcaagaccc gtttagaggc cccaaggggt tatgctacga taccgataga gatgggccaa
     4321 taccacattt gtagaggttt tacttgcttt
//
