LOCUS       sequence-300615-        4356 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for SARS-CoV-2 ORF7b.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4356)
  TITLE     SARS-CoV-2 protein Mammalian expression constructs, tagged, for 
            protein expression and/or purification
REFERENCE   2  (bases 1 to 4356)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4133204
FEATURES             Location/Qualifiers
     source          1..4356
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    64..112
                     /note="synthetic polyadenylation signal"
     terminator      130..176
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     misc_feature    181..252
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(404..540)
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     CDS             complement(join(553..1344,1345..1413))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1176..1195
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2159..2747
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2648..2667
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    2973..3044
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(3089..3225)
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     terminator      complement(3255..3301)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    3416..3537
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3453..3472)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3507..3526
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(3590..3607)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(3608..3628)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     promoter        complement(3832..4034)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(3861..3881)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        4036..4339
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gctatgaact aatgaccccg taattgatta ctagatctgg ctacactcga tcagcagtta
       61 gataataaaa tcgctatcca tcgaagatgg atgtgtgttg gttttttgtg tgtgtaacgc
      121 aacgattgat agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgaatt
      181 gagaggtagc tgaagctgct gttatgacca ctagagggaa gaagatacct gtggagctaa
      241 tggtccaaga tgtcgggagc cgcgatcgct gccaaaaagg taagctgacg atagctgacg
      301 agattcgatg cccgacgagc aaatgagcgc cacggctgtt cgcgtaagct cgaaacgacg
      361 gagattcgat agagccagag tcgagtttaa acgattcggg atcttaagga taaatttctg
      421 gtaaggagga cacgtatgga agtgggcaag ttggggaagc cgtatccgtt gctgaatctg
      481 gcatatgtgg gagtataaga cgcgcagcgt cgcatcaggc atttttttct gcgccaatgc
      541 acctagaatc ggttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
      601 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
      661 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
      721 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
      781 cgcctccatc cagtctatca actgttgccg ggaagctaga gtaagtagtt cgccagttaa
      841 tagtttacgc aaggttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
      901 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
      961 gtgcaaaaaa gcggttagct ccttcggtcc tccaatcgtt gtcagaagta agttggccgc
     1021 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     1081 aagatgcttt tctgtgactg gtgaatactc aaccaagtca ttctgagaat agtgtatgcg
     1141 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     1201 tttaaaagtg ctcatcattg gaaagcgttc ttcggggcga aaactctcaa ggatcttacc
     1261 gctgttgaga tccagttcga tgtaacccac tcgcgcaccc aactgatctt cagcatcttt
     1321 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     1381 aataagggcg acacggaaat gttgaatact catgcgaaag cctcctgctg atcctgtctc
     1441 ttgatagatg tttgcaaaag ctaggactcg attatacgtt gaccgatcag actatcaata
     1501 tctaggttta aacagatcgg tacgctgccg tgaccaggct tgagtacaat atctgacgat
     1561 tggacttcct gggtcccaag ctggattccg actggctgac tgccaagcaa cgatatggat
     1621 cgtcgagact cgcactgaga ggatcgttct cggtacacca ggcgcgcccc gatttcgata
     1681 ttcctcggat ctcggataga tcgggattga ggatgtaggt tgaacttctc tcgggtgatc
     1741 ccgatctcgg atattcggga ttggagtaat acctggcgat aggcgctgcc aagcaatagc
     1801 caaggcgcaa ggccggccaa gagacttgcg atagttcgtt cgagaggtag cctgcaagat
     1861 ggatcggacg aaaggtttga agattttccg acgagggcct tggagagttc ggaaatttaa
     1921 atgcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     1981 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     2041 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     2101 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     2161 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     2221 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     2281 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     2341 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     2401 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     2461 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     2521 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     2581 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     2641 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     2701 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     2761 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgatt taaatgaaac
     2821 tcgtaatcgg agaacgggtg caatagcaag gaccagatcg aacttgactg acgcaattct
     2881 gagcgtagtc gaaacccaga caagtcgatc tcgccaagta gctgacttga ctggttaatt
     2941 aagtttcgat agcccaaggt aaccaatgag attagggagg gaagaaagtt tgatgaacta
     3001 cttctgaccc ctagtggtgt ccagaaaaga ccattaaagg aatgtcttga tcgaaatcga
     3061 gagatcgcct gcaggatagc tgacgacctt aaggataaat ttctggtaag gaggacacgt
     3121 atggaagtgg gcaagttggg gaagccgtat ccgttgctga atctggcata tgtgggagta
     3181 taagacgcgc agcgtcgcat caggcatttt tttctgcgcc aatgccggta gatagcaatc
     3241 cggcccgagg ggcacaaaaa acccctcaag acccgtttag aggccccaag gggttatgct
     3301 acgataccga tagagatggg ccaataccac atttgtagag gttttacttg ctttaaaaaa
     3361 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
     3421 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     3481 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     3541 tgtctgtgag ctgaaggtac gctgtatctc agtcagtcaa gctagcttaa tggtgatggt
     3601 gatggtgcga ctgaaagtaa aggttttcgg cgtgacatgt ctcattatga tcttgtaact
     3661 ccagactgaa ccaaaaaatg attagcataa tgagcaccag aaaaagcagg aaagccaaga
     3721 agcacagata aaagtcgatg agcgatagtt ctatcatggt ggcggccgct ggcgggtgtg
     3781 tcgagtggat ggtaggatcg acaaagatct gatctgacgg ttcactaaac cagctctgct
     3841 tatatagacc tcccaccgta cacgcctacc gcccatttgc gtcaatgggg cggagttgtt
     3901 acgacatttt ggaaagtccc gttgattttg gtgccaaaac aaactcccat tgacgtcaat
     3961 ggggtggaga cttggaaatc cccgtgagtc aaaccgctat ccacgcccat tgatgtactg
     4021 ccaaaaccgc atcagcatgg taatagcgat gactaatacg tagatgtact gccaagtagg
     4081 aaagtcccat aaggtcatgt actgggcata atgccaggcg ggccatttac cgtcattgac
     4141 gtcaataggg ggcgtacttg gcatatgata cacttgatgt actgccaagt gggcagttta
     4201 ccgtaaatac tccacccatt gacgtcaatg gaaagtccct attggcgtta ctatgggaac
     4261 atacgtcatt attgacgtca atgggcgggg gtcgttgggc ggtcagccag gcgggccatt
     4321 taccgtaagt tatgtaacgc ggaactccat atatgg
//
