LOCUS       sequence-300671-        3204 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentivector to express human YTHDF1(264-559) with D401N mutation and
            CRY2olig-BFP at the N-terminal.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3204)
  AUTHORS   Fu Y, Zhuang X
  TITLE     m(6)A-binding YTHDF proteins promote stress granule formation.
  JOURNAL   Nat Chem Biol. 2020 May 25. pii: 10.1038/s41589-020-0524-y. doi: 
            10.1038/s41589-020-0524-y.
  PUBMED    32451507
REFERENCE   2  (bases 1 to 3204)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2020 May 25. pii: 10.1038/s41589-020-0524-y. doi: 
            10.1038/s41589-020-0524-y."
COMMENT     Sequence Label: CRY2olig-BFP-YTHDF1(D401N)-C
FEATURES             Location/Qualifiers
     source          1..3204
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..1494
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTRGAQIMIGAA"
     CDS             1567..2262
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
                     VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
                     LPSKLGHKLN"
ORIGIN
        1 atgaagatgg acaaaaagac tatagtttgg tttagaagag acctaaggat tgaggataat
       61 cctgcattag cagcagctgc tcacgaagga tctgtttttc ctgtcttcat ttggtgtcct
      121 gaagaagaag gacagtttta tcctggaaga gcttcaagat ggtggatgaa acaatcactt
      181 gctcacttat ctcaatcctt gaaggctctt ggatctgacc tcactttaat caaaacccac
      241 aacacgattt cagcgatctt ggattgtatc cgcgttaccg gtgctacaaa agtcgtcttt
      301 aaccacctct atgatcctgt ttcgttagtt cgggaccata ccgtaaagga gaagctggtg
      361 gaacgtggga tctctgtgca aagctacaat ggagatctat tgtatgaacc gtgggagata
      421 tactgcgaaa agggcaaacc ttttacgagt ttcaattctt actggaagaa atgcttagat
      481 atgtcgattg aatccgttat gcttcctcct ccttggcggt tgatgccaat aactgcagcg
      541 gctgaagcga tttgggcgtg ttcgattgaa gaactagggc tggagaatga ggccgagaaa
      601 ccgagcaatg cgttgttaac tagagcttgg tctccaggat ggagcaatgc tgataagtta
      661 ctaaatgagt tcatcgagaa gcagttgata gattatgcaa agaacagcaa gaaagttgtt
      721 gggaattcta cttcactact ttctccgtat ctccatttcg gggaaataag cgtcagacac
      781 gttttccagt gtgcccggat gaaacaaatt atatgggcaa gagataagaa cagtgaagga
      841 gaagaaagtg cagatctttt tcttagggga atcggtttaa gagagtattc tcggtatata
      901 tgtttcaact tcccgtttac tcacgagcaa tcgttgttga gtcatcttcg gtttttccct
      961 tgggatgctg atgttgataa gttcaaggcc tggagacaag gcaggaccgg ttatccgttg
     1021 gtggatgccg gaatgagaga gctttgggct accggatgga tgcataacag aataagagtg
     1081 attgtttcaa gctttgctgt gaagtttctt ctccttccat ggaaatgggg aatgaagtat
     1141 ttctgggata cacttttgga tgctgatttg gaatgtgaca tccttggctg gcagtatatc
     1201 tctgggagta tccccgatgg ccacgagctt gatcgcttgg acaatcccgc gttacaaggc
     1261 gccaaatatg acccagaagg tgagtacata aggcaatggc ttcccgagct tgcgagattg
     1321 ccaactgaat ggatccatca tccatgggac gctcctttaa ccgtactcaa agcttctggt
     1381 gtggaactcg gaacaaacta tgcgaaaccc attgtagaca tcgacacagc tcgtgagcta
     1441 ctagctaaag ctatttcaag aacccgtgga gcacagatca tgatcggagc agcagcccgg
     1501 gatccaccgg tcgccaccga tccaggtggt ggttcaggag gcggcggagg tggaggtgga
     1561 agcggtagcg agctgattaa ggagaacatg cacatgaagc tgtacatgga gggcaccgtg
     1621 gacaaccatc acttcaagtg cacatccgag ggcgaaggca agccctacga gggcacccag
     1681 accatgagaa tcaaggtggt cgagggcggc cctctcccct tcgccttcga catcctggct
     1741 actagcttcc tctacggcag caagaccttc atcaaccaca cccagggcat ccccgacttc
     1801 ttcaagcagt ccttccctga gggcttcaca tgggagagag tcaccacata cgaagacggg
     1861 ggcgtgctga ccgctaccca ggacaccagc ctccaggacg gctgcctcat ctacaacgtc
     1921 aagatcagag gggtgaactt cacatccaac ggccctgtga tgcagaagaa aacactcggc
     1981 tgggaggcct tcaccgagac gctgtacccc gctgacggcg gcctggaagg cagaaacgac
     2041 atggccctga agctcgtggg cgggagccat ctgatcgcaa acatcaagac cacatataga
     2101 tccaagaaac ccgctaagaa cctcaagatg cctggcgtct actatgtgga ctacagactg
     2161 gaaagaatca aggaggccaa caacgagacc tacgtcgagc agcacgaggt ggcagtggcc
     2221 agatactgcg acctccctag caaactgggg cacaagctta atggtggagg tagtggaagt
     2281 ggcggaagcg gaagcctagg cgcgcccata aagcataaca tggacattgg cacctgggat
     2341 aacaaggggc ctgtgccgaa ggccccagtc ccccagcagg caccctctcc acaggctgcc
     2401 ccacagcccc agcaggtggc tcagcctctc ccagcacagc ccccagcttt ggctcaaccg
     2461 cagtatcaga gccctcagca gccaccccag acccgctggg ttgccccacg caacagaaac
     2521 gcggcgtttg ggcagagcgg aggggctggc agcgatagca actctcctgg aaacgtccag
     2581 cctaattctg cccccagcgt cgaatcccac cccgtccttg aaaaactgaa ggctgctcac
     2641 agctacaacc cgaaagagtt tgagtggaat ctgaaaagcg ggcgtgtgtt catcatcaag
     2701 agctactctg aggacaacat ccaccgctcc attaagtact ccatctggtg tagcacagag
     2761 cacggcaaca agcgcctgga cagcgccttc cgctgcatga gcagcaaggg gcccgtctac
     2821 ctgctcttca gcgtcaatgg gagtgggcat ttttgtgggg tggccgagat gaagtccccc
     2881 gtggactacg gcaccagtgc cggggtctgg tctcaggaca agtggaaggg gaagtttgat
     2941 gtccagtgga tttttgttaa ggatgtaccc aataaccagc tccggcacat caggctggag
     3001 aataacgaca acaaaccggt cacaaactcc cgggacaccc aggaggtgcc cttagaaaaa
     3061 gccaagcaag tgctgaaaat tatcagttcc tacaagcaca caacctccat cttcgacgac
     3121 tttgctcact acgagaagcg ccaggaggag gaggaggtgg tgcgcaagga acggcagagt
     3181 cgaaacaaac aacctgcagg ttga
//
