LOCUS       sequence-300678-        8569 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducibly expresses Bcl-xL in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8569)
  AUTHORS   Inde Z, Forcina GC, Denton K, Dixon SJ
  TITLE     Kinetic Heterogeneity of Cancer Cell Fractional Killing.
  JOURNAL   Cell Rep. 2020 Jul 7;32(1):107845. doi: 
            10.1016/j.celrep.2020.107845.
  PUBMED    32640215
REFERENCE   2  (bases 1 to 8569)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2020-07-7"; volume: "32(1):107845. doi"; pages: " 
            10.1016/j.celrep.2020.107845"
COMMENT     Sequence Label: pLenti CMV TRE3G BCL2L1 Neo
FEATURES             Location/Qualifiers
     source          1..8569
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..227
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             228..408
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1073..1306
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1491..1535
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1802..1919
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1946..2324
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     protein_bind    1954..1972
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1990..2008
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2026..2044
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2062..2080
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2098..2116
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2134..2152
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2170..2188
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2233..2257
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    3097..3685
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3150..3170)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3568..3579)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3763..3786)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4113..4132
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4194..4997
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4257..4276)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4867..4886
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             5088..5321
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5393..5514
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5430..5449)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5484..5503
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5554..5689
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5709..5728)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5710..5728)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5738..5755)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5738..5754)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5747..5769)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5896..6351
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5983..6002)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6193..6214
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6377..6481
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6482..6550,6551..7342)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6700..6719)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7513..8101
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8002..8021
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8255..8272
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8389..8410
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8425..8430,8431..8448,8449..8455)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8463..8479
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8468..8490
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8487..8503
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8487..8503
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8522..8542
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8524..8542
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     1741 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcggttaa
     1801 cttttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
     1861 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaatttta
     1921 tcgatcacga gactagcctc gagttggctt tactccctat cagtgataga gaacgtatga
     1981 agagtttact ccctatcagt gatagagaac gtatgcagac tttactccct atcagtgata
     2041 gagaacgtat aaggagttta ctccctatca gtgatagaga acgtatgacc agtttactcc
     2101 ctatcagtga tagagaacgt atctacagtt tactccctat cagtgataga gaacgtatat
     2161 ccagtttact ccctatcagt gatagagaac gtataagctt taggcgtgta cggtgggcgc
     2221 ctataaaagc agagctcgtt tagtgaaccg tcagatcgcc tggagcaatt ccacaacact
     2281 tttgtcttat accaactttc cgtaccactt cctaccctcg taaaccggtt cgtcgactag
     2341 tccagtgtgg tggaattctg cagataccat gtctcagagc aaccgggagc tggtggttga
     2401 ctttctctcc tacaagcttt cccagaaagg atacagctgg agtcagttta gtgatgtgga
     2461 agagaacagg actgaggccc cagaagggac tgaatcggag atggagaccc ccagtgccat
     2521 caatggcaac ccatcctggc acctggcaga cagccccgcg gtgaatggag ccactggcca
     2581 cagcagcagt ttggatgccc gggaggtgat ccccatggca gcagtaaagc aagcgctgag
     2641 ggaggcaggc gacgagtttg aactgcggta ccggcgggca ttcagtgacc tgacatccca
     2701 gctccacatc accccaggga cagcatatca gagctttgaa caggtagtga atgaactctt
     2761 ccgggatggg gtaaactggg gtcgcattgt ggcctttttc tccttcggcg gggcactgtg
     2821 cgtggaaagc gtagacaagg agatgcaggt attggtgagt cggatcgcag cttggatggc
     2881 cacttacctg aatgaccacc tagagccttg gatccaggag aacggcggct gggatacttt
     2941 tgtggaactc tatgggaaca atgcagcagc cgagagccga aagggccagg aacgcttcaa
     3001 ccgctggttc ctgacgggca tgactgtggc cggcgtggtt ctgctgggct cactcttcag
     3061 tcggaaatga atccagcaca gtggcggccg ctcgacaatc aacctctgga ttacaaaatt
     3121 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     3181 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     3241 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     3301 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     3361 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     3421 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     3481 ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg
     3541 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     3601 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     3661 atctcccttt gggccgcctc cccgcctgga attctgcaga tatacggact agtgatctaa
     3721 ttctaccggg taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc
     3781 ccgctgggca cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc
     3841 ggtaggcgcc aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc
     3901 ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa
     3961 gtagcacgtc tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta
     4021 ggcctttggg gcagcggcca atagcagctt tgctccttcg ctttctgggc tcaggggcgg
     4081 ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc
     4141 tgccgcgctg ttctcctctt cctcatctcc gggcctttcg acctgcagcc aatatgggat
     4201 cggccattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
     4261 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
     4321 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
     4381 tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
     4441 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
     4501 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
     4561 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
     4621 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
     4681 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg
     4741 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
     4801 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
     4861 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
     4921 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
     4981 ttgacgagtt cttctgaggg gatccgtcga gggcccggta cctttaagac caatgactta
     5041 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctagc
     5101 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca
     5161 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     5221 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     5281 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct
     5341 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt
     5401 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     5461 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     5521 ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     5581 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     5641 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct agggacgtac
     5701 ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc
     5761 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
     5821 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
     5881 tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     5941 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     6001 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     6061 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     6121 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     6181 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     6241 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     6301 tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac
     6361 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     6421 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     6481 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     6541 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     6601 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     6661 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     6721 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     6781 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     6841 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     6901 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     6961 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     7021 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     7081 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     7141 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     7201 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     7261 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     7321 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     7381 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     7441 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     7501 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     7561 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     7621 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     7681 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     7741 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     7801 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     7861 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     7921 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     7981 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     8041 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     8101 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     8161 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     8221 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     8281 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     8341 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
     8401 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
     8461 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg
     8521 cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaa
//
