LOCUS       sequence-30068-e         691 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Fuerer C, Nusse R
  TITLE     Lentiviral vectors to probe and manipulate the wnt signaling 
            pathway.
  JOURNAL   PLoS One. 2010  . 5(2):e9370.
  PUBMED    20186325
REFERENCE   2  (bases 1 to 691)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2010  . 5(2):e9370."
COMMENT     Sequence Label: E[beta]C
FEATURES             Location/Qualifiers
     source          1..691
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     18..37
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        18..36
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             162..197
                     /codon_start=1
                     /product="nanobody epitope tag corresponding to amino acids
                     16-27 of human beta-catenin (Traenkle et al., 2015)"
                     /label=BC2 tag
                     /translation="PDRKAAVSHWQQ"
ORIGIN
        1 gaggattagc ttggtactaa tacgactcac tatagggaga cccaagctgg ctaggtaagc
       61 ttggtaccga gctcggatcc actagtccag tgtggtggaa ttctgcagat aacaccatgg
      121 ctactcaagc tgacctgatg gagttggaca tggccatgga gccggacaga aaagctgctg
      181 tcagccactg gcagcagcag tcttacttgg atgctggaat ccatgctggt gccaccgcca
      241 cagctcctgc cctgagtggc aagggcaacc ctgaggaaga agatgttgac acctcccaag
      301 tcctttatga atgggagcaa ggcttttccc agtccttcac gcaagagcaa gtagctgata
      361 ttgacgggca gtatgcaatg actagggctc agagggtccg agctgccatg ttccctgaga
      421 cgctagatga gggcatgcag atcccatcca cgcagtttga cgctgctcat cccactaatg
      481 tccagcgctt ggctgaacca tcacagatgt tgaaacatgc agttgtcaat ttgattaact
      541 atcaggatga cgcggaactt gccacacgtg caattcctga gctgacaaaa ctgctaaacg
      601 atgaggacca ggtggtagtt aataaagctg ctgttatggt ccatcagctt tccaaaaagg
      661 aagcttccag acatgccatc atgcgctccc c
//
