LOCUS       sequence-300682-       15732 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Integrating the full-length tetO array (from pLAU44) near the his-3 
            gene in N. crassa.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15732)
  AUTHORS   Nguyen TS, Gladyshev E
  TITLE     Developing a tetO/TetR system in Neurospora crassa.
  JOURNAL   Fungal Genet Biol. 2020 Mar;136:103316. doi: 
            10.1016/j.fgb.2019.103316. Epub 2019 Dec 9.
  PUBMED    31821884
REFERENCE   2  (bases 1 to 15732)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet Biol."; date: "2020-03"; volume: "136:103316. doi"; pages: " 
            10.1016/j.fgb.2019.103316. Epub 2019 Dec 9"
COMMENT     Sequence Label: pTSN6
FEATURES             Location/Qualifiers
     source          1..15732
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(98..117)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     217..239
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(277..295)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        363..467
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(468..536,537..1328)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(686..705)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1499..2087
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1988..2007
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2241..2258
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        2345..2363
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2638..2649
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4007..4025
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    4100..4118
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4129..4147
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4191..4209
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4267..4285
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4296..4314
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4325..4343
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4372..4390
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4400..4418
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4429..4447
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4476..4494
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4533..4551
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4580..4598
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4609..4627
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4638..4656
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4707..4725
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4736..4754
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4783..4801
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4812..4830
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4841..4859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4888..4906
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4917..4935
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4946..4964
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5049..5067
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5096..5114
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5152..5170
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5199..5217
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5228..5246
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5257..5275
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5304..5322
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5333..5351
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5362..5380
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5409..5427
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5438..5456
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5467..5485
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5514..5532
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5543..5561
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5573..5591
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5677..5695
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5724..5742
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5753..5771
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5782..5800
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5829..5847
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5887..5905
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5934..5952
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5963..5981
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5992..6010
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6038..6056
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6067..6085
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6096..6114
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6143..6161
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6201..6219
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6249..6267
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6306..6324
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6353..6371
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6395..6413
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6471..6489
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6547..6565
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6576..6594
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6605..6623
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6652..6670
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6681..6699
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6710..6728
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6757..6775
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6786..6804
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6815..6833
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6862..6880
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6891..6909
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6994..7012
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7023..7041
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7097..7115
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7126..7144
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7173..7191
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7278..7296
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7307..7325
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7336..7354
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7383..7401
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7412..7430
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7441..7459
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7488..7506
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7517..7535
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7546..7564
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7593..7611
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7621..7639
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7650..7668
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7726..7744
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7755..7773
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7802..7820
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7831..7849
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7860..7878
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7906..7924
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7935..7953
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7982..8000
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8011..8029
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        join(8081..8086,8087..8103,8104..8109)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             8298..8831
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     protein_bind    8849..8867
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8878..8896
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8984..9002
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9013..9031
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9060..9078
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9089..9107
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9118..9136
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9194..9212
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9223..9241
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9270..9288
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9299..9317
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9328..9346
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9375..9393
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9404..9422
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9433..9451
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9480..9498
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9537..9555
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9584..9602
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9613..9631
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9642..9660
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9689..9707
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9821..9839
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9888..9906
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9917..9935
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9946..9964
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9993..10011
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10022..10040
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10051..10069
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10098..10116
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10127..10145
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10156..10174
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10259..10277
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10306..10324
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10335..10353
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10410..10428
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10439..10457
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10468..10486
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10515..10533
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10544..10562
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10638..10656
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10667..10685
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10714..10732
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10743..10761
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10772..10790
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10819..10837
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10848..10866
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10877..10895
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10924..10942
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10953..10971
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10982..11000
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11058..11076
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11133..11151
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11162..11180
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11191..11209
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11238..11256
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11267..11285
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11296..11314
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11371..11389
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11400..11418
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11447..11465
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11476..11494
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11505..11523
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11552..11570
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11581..11599
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11610..11628
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11657..11675
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11686..11704
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11715..11733
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11762..11780
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11818..11836
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11865..11883
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11894..11912
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11923..11941
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11970..11988
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11999..12017
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    12028..12046
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    12075..12093
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    12104..12122
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    12133..12151
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    12180..12198
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    12209..12227
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    12238..12256
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    12285..12303
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    12400..12418
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    12476..12494
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    12505..12523
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    12534..12552
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    12581..12599
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    12610..12628
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    12639..12657
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    12686..12704
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    12715..12733
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    12791..12809
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    12820..12838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    12849..12867
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    12896..12914
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    12925..12943
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    12954..12972
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(13011..13027)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13012..13028)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(join(15731..15732,1..17))
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(join(15732,1..17))
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 tatagtgtca cctaaatcgt atgtgtatga tacataaggt tatgtattaa ttgtagccgc
       61 gttctaacga caatatgtcc atatggtgca ctctcagtac aatctgctct gatgccgcat
      121 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
      181 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
      241 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
      301 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
      361 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
      421 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
      481 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
      541 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
      601 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
      661 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
      721 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
      781 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
      841 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
      901 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
      961 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     1021 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     1081 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     1141 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     1201 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     1261 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     1321 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     1381 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     1441 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     1501 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     1561 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     1621 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     1681 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     1741 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     1801 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     1861 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     1921 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     1981 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     2041 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     2101 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     2161 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     2221 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     2281 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg caggttaacc tggcttatcg
     2341 aaattaatac gactcactat agggagaccg gcagatccaa tgcggatgga ttcgctcact
     2401 cggtgccgcg gcggtgcgaa gttcctgccg tggattgggg gattgcacag acggtggagt
     2461 gcttgctaca gctaggtacc cagttgcaca ccaaacggtg cccctgtgcc cctgcgtcta
     2521 ggtgtgtcct gtgagcaact gcgcccgcgc gcggcaccgg gaagctgcgg gaacctcaat
     2581 ggatgattgg cgccagatcc ccggcatcac ctcagcacat gggcagcccg agaaggaatc
     2641 gagggtagaa gatattccag ggtttccgct agcacaactt ccaagcgcct ctaccgctac
     2701 tccacacacc cacgctgaat ggcacaacca ctgaggccta gcggtcccag ctccccgtaa
     2761 ccacgcagcc ccagcgaccg aggtcatcat gacactatga cgacaatctg gcgggagact
     2821 tgggaaaatc gatgacgggc acctaccttg ggaacaatag agcaagtcca gtcaacgccc
     2881 tagggaaggg ggaaaaaaaa aaaagagccc attctcacaa ggagcaacac tgtgctgcta
     2941 tcatagtaca ctacaagcaa agtcgggatg ttgctagaat ccgtcggcta cgtcggtaga
     3001 tacctcccat aggcagtaag tctaggtatc caatttatag ttccgcgtat ttcttcgtcg
     3061 aacggggcac agcaaagcct aacagccgca gtgcctcgca accaacacca agcagtaatc
     3121 tcatccatcc atacagatcc tcccacaata tctttacatg cgcacttgcg ctgtgttagc
     3181 agtttaggtt ccactaccaa ggctgacacg agcagcactg acagcaaact gctgcttggg
     3241 gccaagaaat tggcagtttg ctgagcacct acatatcagt acacatcaac cctggtttaa
     3301 ttgttagcct ggtactgtta accgtcatga acgaggacgt ggaggaagaa ccacgctaac
     3361 aattgaacca gggttgatgt atacctctct taccatattt gcttgcttcc acgtccctat
     3421 cctcaagcga ttttgttctt gactttttgt atttcaacgc aaaggccatt caggtttctc
     3481 atgttcaacc ctttggatgg cgatgccgtt cgtcccagga cctcgtgacc tttgaaagac
     3541 gagaaatata tcccaaagca acttctgctt atggacaagc aactgcatgg cggcccacaa
     3601 ctcatatctc gcgtaatcat ccaaacgcta ggcccgagta ctctactgtt ctcgagacac
     3661 ctaccataat atgtacccaa tcagacatga caacagtcaa ccaaacatac cctgatccgg
     3721 ccaaaccctg acatccctag atgatggtta ggcacacaaa tggaaaacgg gactcgttgg
     3781 ttaggtggga acgcttgtat tgcagccagc caaccgactc tcctcgtccc tctcgagtcc
     3841 cgttattgcc gtttgaccca gccgcccagc cgccaaacag ctgagggagc caatggtcta
     3901 atacaccgtc caacggcccc agtgatgttt caaacttgat tgacagcgaa cgaaacccct
     3961 gaaacccgtt atagatatgt atagatttga cggggtagct tggccctaat acgactcact
     4021 atagggcgaa ttggagctcc accgcggtgg cggccgcctc tagcagggag gtcctatcag
     4081 tgatagagaa gacgaaccgt ccctatcagt gatagagagg ggaaaggatc cctatcagtg
     4141 atagagacga agtgggactc tagcacggag gcgggtccct atcttgcgcg tccctatcag
     4201 tgatagagaa gtggggaggt ccctatcagt gataaagaca gtgtgtggct ctagcagcgg
     4261 gcagggtccc tatcagtgat agagagaaca ggagatccct atcagtgata gagacgacgg
     4321 aaggtcccta tcagtgatag agaaaggtaa tcgctctagc aagcgagggg gtccctatca
     4381 gtgatagaga gacaggtggt ccctatcagt gatagagaaa ggagacggtc cctatcagtg
     4441 atagagaatg gggccagctc tagcagaagg gaaggtccct atcagtgata gagagaccta
     4501 tagatcctat cagtgataga gaacaggatg gatccctatc agtgatagag aagggacgaa
     4561 gctctagcag gggagacggt ccctatcagt gatagagagg cgcgcttgtc cctatcagtg
     4621 atagagagac gccggagtcc ctatcagtga tagagagaag ggacaactct agcaatggtc
     4681 cctacagtga tagagaacag aacgcatccc tatcagtgat agagactgcg gactatccct
     4741 atcagtgata gagacggggt gcatctctag cagggccgag aatccctatc agtgatagag
     4801 aatgcggggc ctccctatca gtgatagaga gccgaaatgt tccctatcag tgatagagaa
     4861 gcgagctggc tctagcagag ggcacaatcc ctatcagtga tagagagacg ggaccgtccc
     4921 tatcagtgat agagataaag ccgagtccct atcagtgata gagatggaga gagactctag
     4981 cagaaagagt tgtccctatc agtgacagag aaagtcagag gtccctcagt gatagagagg
     5041 gggagatgtc cctatcagtg atagagacag atcagcactc tagcatgaag tgtagtccct
     5101 atcagtgata gagacactca ggaatccctc agtgatagag aatctcgggt gtccctatca
     5161 gtgatagaga gggggctact ctctagcagg gtctctagtc cctatcagtg atagagagag
     5221 cgcagactcc ctatcagtga tagagacgaa gagccatccc tatcagtgat agagacaagg
     5281 gataactcta gcaatgggga ccatccctat cagtgataga gatggcggcg gttccctatc
     5341 agtgatagag acgcaggcgg atccctatca gtgatagaga agtaaggagg ctctagcatg
     5401 gagtctgatc cctatcagtg atagagaccg ggagacatcc ctatcagtga tagagagcgg
     5461 tttaaatccc tatcagtgat agagaaccgc ggggactcta gcacggagcc gtgtccctat
     5521 cagtgataga gaagagagga ggtccctatc agtgatagag acgtggagaa ggtccctatc
     5581 agtgatagag agaatggtag gctctagcag aggccgctat ccctatagtg atagagaatt
     5641 atacacgtcc ctatcagtga tagggaggaa gatgtatccc tatcagtgat agagagtgga
     5701 aggtactcta gcagagagag gcgtccctat cagtgataga gaggaagtaa gctccctatc
     5761 agtgatagag agagtgctaa gtccctatca gtgatagaga aagagacgga ctctagcatg
     5821 agagcgactc cctatcagtg atagagacgc ggagtgatcc ctattagtga tagagacgag
     5881 gggggatccc tatcagtgat agagagcctt ataatctcta gcaggggagc taatccctat
     5941 cagtgataga gagtcatata aatccctatc agtgatagag agcgagccga ctccctatca
     6001 gtgatagaga ccgttaaaaa ctctagcagg tgaggtatcc ctatcagtga tagagaacaa
     6061 cgaaaatccc tatcagtgat agagagggaa aaaagtccct atcagtgata gagaggcggg
     6121 gagactctag cattgcaact agtccctatc agtgatagag agagacaaac gtccctacca
     6181 gtgatagaga ttccggagag tccctatcag tgatagagac gaaaaagagt ctctagcacg
     6241 gggggatgtc cctatcagtg atagagagag gcctgagtcc tatcagtgat agagaaagca
     6301 cagggtccct atcagtgata gagagggtag agcgctctag caggtgccaa cgtccctatc
     6361 agtgatagag agcgcggcag gtccctatca ggaatcccta tcagtgatag agagggcggg
     6421 gagctctagc agggccaaac gtccctctca gtgatagaga agggccaaaa tccctatcag
     6481 tgatagagat ataaaagcgt ccctatcagt ggtagagagg aagcagcact ctagcagcat
     6541 aaatgatccc tatcagtgat agagagagag gcggctccct atcagtgata gagaaaaggg
     6601 ccggtcccta tcagtgatag agaatgcagg gagctctagc agaatgacag gtccctatca
     6661 gtgatagaga gggcgatgcc tccctatcag tgatagagag cgccgggggt ccctatcagt
     6721 gatagagaag aatagtagct ctagcagaag gaataatccc tatcagtgat agagaaggca
     6781 atggctccct atcagtgata gagaagcacg catatcccta tcagtgatag agaggcgcgg
     6841 gcactctagc aacggcggag atccctatca gtgatagaga ggagaccaca tccctatcag
     6901 tgatagagaa tgagggggat ccctatagtg atagagagga gggcagcctc tagcagtatg
     6961 gacgatccct atcaggatag agaaaggggg aactccctat cagtgataga gagtaaggtc
     7021 gttccctatc agtgatagag agggcctggg actctagcag tggtagctgt ccctaagtga
     7081 tagagaaacc agaatatccc tatcagtgat agagaaagag atggttccct atcagtgata
     7141 gagaaagggc cacactctag caaagtagca gttccctatc agtgatagag aaagttgttg
     7201 atccctatct gtggtagaga gagcagacca cccctatcag tgatagagag ggaagggccc
     7261 tctagcagac gggggaatcc ctatcagtga tagagataaa cgctcttccc tatcagtgat
     7321 agagacctag cagggtccct atcagtgata gagaccccct agtgctctag caacgaaaca
     7381 aatccctatc agtgatagag aaggagtggt atccctatca gtgatagaga gcaggggagg
     7441 tccctatcag tgatagagaa aacgcatcac tctagcacgg ccgcgtgtcc ctatcagtga
     7501 tagagaagcg gcggaatccc tatcagtgat agagacgacg tgcagtccct atcagtgata
     7561 gagaaaagaa gccactctag caggcatggg ggtccctatc agtgatagag agagatagcg
     7621 tccctatcag tgatagagac ggcgttccgt ccctatcagt gatagagaaa accgggcact
     7681 ctagcaagag agcgagaccc tatcagtgat agagatcggg acgtatccct atcagtgata
     7741 gagatgaggg ggactcccta tcagtgatag agataaatat agactctagc aaggcatgac
     7801 atccctatca gtgatagaga tggaaaaact tccctatcag tgatagagaa agggacaatt
     7861 ccctatcagt gatagagagg tactggcact ctagcacgcg ggtgctccct atcagtgata
     7921 gagaacgcag caggtcccta tcagtgatag agaagatggg ggcctctagc aaaaaatgcg
     7981 gtccctatca gtgatagaga gacaagaagt tccctatcag tgatagagac aggggcactt
     8041 ccctatcgtg atagagatga gagactactc tagcacccag ttgacataag cctgttcggt
     8101 tcgtaaactg taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga
     8161 acgcagcggt ggtaacggcg cagtggcggt tttcatggct tgttatgact gtttttttgt
     8221 acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc gatgtttgat
     8281 gttatggagc agcaacgatg ttacgcagca gcaacgatgt tacgcagcag ggcagtcgcc
     8341 ctaaaacaaa gttaggtggc tcaagtatgg gcatcattcg cacatgtagg ctcggccctg
     8401 accaagtcaa atccatgcgg gctgctcttg atcttttcgg tcgtgagttc ggagacgtag
     8461 ccacctactc ccaacatcag ccggactccg attacctcgg gaacttgctc cgtagtaaga
     8521 cattcatcgc gcttgctgcc ttcgaccaag aagcggttgt tggcgctctc gcggcttacg
     8581 ttctgcccaa gtttgagcag ccgcgtagtg agatctatat ctatgatctc gcagtctccg
     8641 gcgagcaccg gaggcagggc attgccaccg cgctcatcaa tctcctcaag catgaggcca
     8701 acgcgcttgg tgcttatgtg atctacgtgc aagcagatta cggtgacgat cccgcagtgg
     8761 ctctctatac aaagttgggc atacgggaag aagtgatgca ctttgatatc gacccaagta
     8821 ccgccaccta atctagcact ggcggcggtc cctatcagtg atagagatgg agttggatcc
     8881 ctatcagtga tagagactaa agggggtccc tatcagtgat ggagaaggta atacgctcta
     8941 gcaaagacga gagtccctat cagtgataga gggaggtgtg gaatccctat cagtgataga
     9001 gagtaaaacg gatccctatc agtgatagag aacgcagtag actctagcag gctttgaagt
     9061 ccctatcagt gatagagaaa tccagtggtc cctatcagtg atagagaagg aagtagttcc
     9121 ctatcagtga tagagaatgg cagaggctct agcagagacg gcaatcccca tcagtgatag
     9181 agaggatcag aattccctat cagtgataga gaaatatggc aatccctatc agtgatagag
     9241 atcatagcgg cctctagcag agttgggggt ccctatcagt gatagagagt atgtagtatc
     9301 cctatcagtg atagagatag agaggaatcc ctatcagtga tagagaggga gaagagctct
     9361 agcaaggggg tacgtcccta tcagtgatag agataggggg aggtccctat cagtgataga
     9421 gagcgagggg aatccctatc agtgatagag aaggggactc actctagcaa cgatacaggt
     9481 ccctatcagt gatagagagg atagccagtc cctatcagta tagagaactg ggcaaatccc
     9541 tatcagtgat agagaaaatg gcatgctcta gcagaaaatc aagtccctat cagtgataga
     9601 gaggtgagga gctccctatc agtgatagag agggttatca gtccctatca gtgatagaga
     9661 aaaatagagt ctctagcagg gtaagtcgtc cctatcagtg atagagaaga ataagtatcc
     9721 ctatcagtga tagagccaaa gcatgatccc tatagtgata gagatggatt caactctagc
     9781 accggaaaag ttccctatca gtggtagaga agctgaaaag tccctatcag tgatagagag
     9841 gcgaatgact ccctatcagt gaggagcggc tctagcaaag agaggagtcc ctatcagtga
     9901 tagagatcag aggcactccc tatcagtgat agagaggggg atacatccct atcagtgata
     9961 gagaaagcgt tggactctag cagcgaagcg attccctatc agtgatagag aaaggagagg
    10021 atccctatca gtgatagaga gtggggcgca tccctatcag tgatagagac gaggtccagc
    10081 tctagcaagc aggtccatcc ctatcagtga tagagagaag gggaggtccc tatcagtgat
    10141 agagaaaagg atggatccct atcagtgata gagagcgatc ggaactctag cagactaaga
    10201 gttcctatca gtgatagaga caagaaagca tccctaacag tgatagagag agcgcaagtc
    10261 cctatcagtg atagagagac agggcagctc tagcacgcgt aaggatccct atcagtgata
    10321 gagatcggtg aatatcccta tcagtgatag agacgggagg gcctcctatc agtgatagag
    10381 atacatagag gctctagcaa aagcgaacct ccctatcagt gatagagagg tgcgtgaatc
    10441 cctatcagtg atagagaaag ccagcgttcc ctatcagtga tagagaggca aacatgctct
    10501 agcagaaagt ttactcccta tcagtgatag agaagaaagg acttccctat cagtgataga
    10561 gagaaaagcg actcagtgat agagaaggac gaaatctcta gcagtcaatg ggctcccttg
    10621 atagagaaaa tacgggttcc ctatcagtga tagagaggag aggggctccc tatcagtgat
    10681 agagagcacg caggactcta gcacgtaaag tgctccctat cagtgataga gaagcgggaa
    10741 aatccctatc agtgatagag agctacagag ctccctatca gtgatagaga tctcagaggg
    10801 ctctagcaaa cgggagagtc cctatcagtg atagagagag ttaagactcc ctatcagtga
    10861 tagagacgca tcacggtccc tatcagtgat agagacacgt ttgaactcta gcagaagtgc
    10921 ggatccctat cagtgataga gactaaagaa tttccctatc agtgatagag aacattcacg
    10981 ttccctatca gtgatagaga gtgtgggcgc ctctagcatc gaggggaatc cctatgagtg
    11041 atagagacgg agataagtcc ctatcagtga tagagacgat atgagtccct atcagtggta
    11101 gagaggggac atcgctctag cagtgggatg gatccctatc agtgatagag aacacaggcc
    11161 atccctatca gtgatagaga ggcgtaacac tccctatcag tgatagagat acagacaatc
    11221 tctagcaagc atgaggctcc ctatcagtga tagagagcgg acgggctccc tatcagtgat
    11281 agagagtagg ggccatccct atcagtgata gagagaaaaa ggaactctag cactcgggat
    11341 ggtccttatc agtgatagag agaatatagg tccctatcag tgatagagag ccgtcacgct
    11401 ccctatcagt gatagagaga gggggtgcct ctagcactcc gcgggctccc tatcagtgat
    11461 agagaggcaa ggaagtccct atcagtgata gagaaatcga tcgttcccta tcagtgatag
    11521 agatgggtct aaactctagc atagcagaag ctccctatca gtgatagaga ggggggagat
    11581 tccctatcag tgatagagag acaggaaaat ccctatcagt gatagagacg ctacagcact
    11641 ctagcacaaa ccctggtccc tatcagtgat agagagggat cgtaatccct atcagtgata
    11701 gagaagagca tggatcccta tcagtgatag agagcgcgca gggctctagc acgtcatggt
    11761 atccctatca gtgatagaga gcacgcagac cctatcagtg atagagagag gcaggggtcc
    11821 ctatcagtga tagagacagg tcccgactct agcatcgtga caagtcccta tcagtgatag
    11881 agaaccccga gcctccctat cagtgataga gagaatcaga ggtccctatc agtgatagag
    11941 aaacacgggt cctctagcaa gaagatcaat ccctatcagt gatagagaaa aggtgagatc
    12001 cctatcagtg atagagactc agcgctgtcc ctatcagtga tagagaatgc agggagctct
    12061 agcagaatga caggtcccta tcagtgatag agacccaagg tcgtccctat cagtgataga
    12121 gagctgtgca tctccctatc agtgatagag accgcaagaa gctctagcag aagcagttgt
    12181 ccctatcagt gatagagaag cggaaccatc cctatcagtg atagagaaga atagaagtcc
    12241 ctatcagtga tagagagata taccaactct agcaaaggcc gggatcccta tcagtgatag
    12301 agacagcacg gagaatgcag cgcctcccta tcagtgaaag gagaagaagt ggctctagca
    12361 aggcccgggt tccctatcag ttatagagaa ggtgagcggt ccctatcagt gatagagaga
    12421 acacgaaatc cctatctgtg atagagagca agggggtctc tagcagcgga ggcagtccct
    12481 atcagtgata gagactgggg acgatcccta tcagtgatag agagagtcgc ggttccctat
    12541 cagtgataga gaaacgcagg tgctctagca atgcatatta tccctatcag tgatagagaa
    12601 aggcgaggct ccctatcagt gatagagatg acgaaagatc cctatcagtg atagagacgg
    12661 agccaaactc tagcagtggg tggggtccct atcagtgata gagagaggga agggtcccta
    12721 tcagtgatag agaggaagag aggtccctgt cagtgataga gaggtgagac cactctagca
    12781 agagtgggga tccctatcag tgatagagaa cgttgatcat ccctatcagt gatagagaag
    12841 agtgaaagtc cctatcagtg atagagaagc accgcccctc tagcacggcc ggccatccct
    12901 atcagtgata gagagggcga cgtgtcccta tcagtgatag agaaatctag caatccctat
    12961 cagtgataga gagacggacg tactctagac gaattcgata tcaagcttat cgataccgtc
    13021 gacctcgagg gggggcccgg taccagcttt tgttcccttt agtgagggtt aatagggcgt
    13081 gcacggctat ggggtctgaa tgctaaaaga caccatttcc cacactccct ctgatttttt
    13141 ttatggtttt tttttatctt catttttcgc cgtcttttat tcggttgttt gggaggttca
    13201 catgcaacgt gcaagactgc aagtatattg agatgggcgc ttcatgcaat gcagcatcac
    13261 tgcagcgaca ttgtgacatc cacgtttcga tcgtctgtgc ttccccgttc atgtacgcat
    13321 gatctgaaaa aaaaaaaaaa aaaaaaaaag ttgatgtaat aatgaaaata aacacccagg
    13381 gacgagcacc ccttctggaa tagggagaaa aactaaaggg acgtttgggt ctctacactt
    13441 accaacagat tcatgtactg atcttcagcc tgtacagaac agtacaatgt ccaaaactcg
    13501 ttatcattac tctatgtgcc caaaatgacc cagatataca tcaacccact acatcctttt
    13561 gttcttgttt tttttttttt tttttgctac ttcgaaaaga atttccgttt agttgctctt
    13621 gctcatgtgc tcaagacgga tgctgaccgc ccttctgtga gcctcgagct cctcaacctt
    13681 agccaactgc atgacagcct ggccgacgtt tttgagaccc tcggcagtca agttggagct
    13741 ggtaatgtgc ttgacgaacg aggcgagatt gacgccagag tactgcttgg caaagccata
    13801 ggtagctatg tgtcttgacg ttagcagaag aatctgtgaa gcggggctat ttatgatatg
    13861 taacttacgc agcgagtggt taacaccagc agagtaatcg ccaacggact caggagtcca
    13921 ggcgccaatg aagacactac cggcgttcat gacaagatcg acagccttct cggcctcctt
    13981 gatctggagg atcaagtgct caggagcgta cttgttgctg agctccatgg cctcctcgac
    14041 ggtcttgacc tgcacggtga tcgagtgggc gatggagcca cggacaatct ggacgcgagg
    14101 aagctccgta gcctgacggt gaacctcgtc ctcaatagcc tgaagatgct cctcgtcgag
    14161 gtcaatagcg atcaggatga cctgactgtc aacgccgtgc tcagcctggg acaggagatc
    14221 cgaggcaacg aacgcggggt tggcgtcctt gtcagcgatg accagcacct cggacgggcc
    14281 agcgggcatg tcaataccaa cggcagcgtt ggtgtcgttg ctgacgaaca tcttggcagc
    14341 agtgacgaac tggttaccgg ggccgagaat cttgtcgacc ttggtgatgc tctcggtgcc
    14401 gtaggccatg gcagctacgg cctgggcacc gccggcaagc acgatggact cggcgccaac
    14461 cttgtgagcg acgtagacaa tctcgggagt gatggttccg tcggcgcggg gaggagaggc
    14521 gaacacaatc ttgttgcagc cggcgaccat ggcgggaaca cccagcataa gggcagtgct
    14581 ggggagaacg gcggtaccgc cggggatgta gcagccgacg gcctcgatgg gacgagagaa
    14641 acggctgcag acaacaccgg gcatggtctc gacctggagg ggcttctcct ccttctgggc
    14701 ggcgtggaac ttgcggatgt tctcgaagga cacgtcgatg gcagcaatgg tctcctcagg
    14761 gagctgcata agctccttgg ggaagggcgc cttcaggacg gggctagtaa gagaggtagc
    14821 cttctcgaac ttgtgagtgt acgacagaac agccttgtcg ccgttcttgc ggacgtcctc
    14881 gatgatgggg acaatgatct tgtagatggc atcggacgac ttttgcgcag gacgcttgag
    14941 agcagcatcg agctcctcgg tagagacctt ggaggcgtcg aaacgtctca tggtgatctt
    15001 ctcaggggtg gtctcctggg cggcctcctt ggtgacaggg gccgaagtgg cagcgagagc
    15061 ggacgccgca ggcttgatgc cctccttctc agcccactta cccttagcat ctccagtcct
    15121 gcgcttgacc ttccagctct tggcgtcaag gctcctttcg atatcggcaa gagtaacgcc
    15181 ggcagcaacg gccctggtaa gagcaaagta gaagagatcg gcagcctcaa aggcgatttc
    15241 ctggggggtc tgagcggtgc agagctcctc agcctcctcc atgatcttgg cccggactag
    15301 cttctcatcg gagaagagac gggcagtgta ggagccctcg ggggcagact gtttcctcga
    15361 aatcaaagtc tgctcgagct tgggaaggcc tttgagctga ccaaagcagc cggactgatc
    15421 gaggtggcag aaacgaccct tctgcttcac gacaaacttg agagcatcgt tatcgcagtc
    15481 aagcgagatg cggacgagct cctgagtgtc tccggaagta gcacccttgt accagagacc
    15541 gcgcttccgg ctctgataga caccagtctg tgtcctgagg gcctcgttca cactctcggc
    15601 actgctgtag accagaccca gagcagtgtc gtgctcatca acgacaacgg tggggagaag
    15661 accatcggga cggtcagact tccagacgct cgagaggatg gtggagatgg caagcttcag
    15721 ctgctcgagt tc
//
