LOCUS       sequence-30070-e         779 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Brugmann SA, Goodnough LH, Gregorieff A, Leucht P, ten Berge D, 
            Fuerer C, Clevers H, Nusse R, Helms JA
  TITLE     Wnt signaling mediates regional specification in the vertebrate 
            face.
  JOURNAL   Development. 2007 Sep . 134(18):3283-95.
  PUBMED    17699607
REFERENCE   2  (bases 1 to 779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development. 2007 Sep . 134(18):3283-95."
COMMENT     Sequence Label: 7TG
FEATURES             Location/Qualifiers
     source          1..779
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(84..115)
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     misc_feature    337..454
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(721..765)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gctcctcgcc cttgctcacc atggtggctt taccaacagt accggaatgc caagcttttt
       61 accaacagta ccggaatgcc aagctggaat tcgagcttcc attatatacc ctctagagtc
      121 tagatctcga gcccgggcta gcacgcgggc gcgccccttt gatcttaccc cctttgatct
      181 tacccccttt gatcttaccc cctttgatct cgcgcccctt tgatcttacc ccctttgatc
      241 ttaccccctt tgatctcgcg taagagctcg gtaccctgca ggcatgcaag cttcagctgc
      301 tcgagcccgg gctaggaggc tagtctcgtg atcgataaaa ttttgaattt ttgtaatttg
      361 tttttgtaat tctttagttt gtatgtctgt tgctattatg tctactattc tttcccctgc
      421 actgtacccc ccaatccccc cttttctttt aaaagttaac cgataccgtc gagatccgtt
      481 cactaatcga atggatctgt ctctgtctct ctctccacct tcttcttcta ttccttcggg
      541 cctgtcgggt cccctcgggg ttgggaggtg ggtctgaaac gataatggtg aatatccctg
      601 cctaactcta ttcactatag aaagtacagc aaaaactatt cttaaaccta ccaagcctcc
      661 tactatcatt atgaataatt ttatatacca cagccaattt gttatgttaa accaattcca
      721 caaacttgcc catttatcta attccaataa ttcttgttca ttcttttctt gctggtttt
//
