LOCUS       sequence-300714-        4434 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for SARS-CoV-2 ORF6 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4434)
  TITLE     SARS-CoV-2 protein Mammalian expression constructs, tagged, for 
            protein expression and/or purification
REFERENCE   2  (bases 1 to 4434)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4133931
FEATURES             Location/Qualifiers
     source          1..4434
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    23..71
                     /note="synthetic polyadenylation signal"
     terminator      89..135
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     misc_feature    140..211
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(363..499)
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     CDS             complement(join(512..1303,1304..1372))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1135..1154
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2118..2706
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2607..2626
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    2932..3003
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(3048..3184)
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     terminator      complement(3214..3260)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    3375..3496
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3412..3431)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3466..3485
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(3549..3590)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             complement(3591..3611)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     promoter        complement(3869..4071)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(3898..3918)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        4073..4376
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tacactcgat cagcagttag ataataaaat cgctatccat cgaagatgga tgtgtgttgg
       61 ttttttgtgt gtgtaacgca acgattgata gcataacccc ttggggcctc taaacgggtc
      121 ttgaggggtt ttttgaattg agaggtagct gaagctgctg ttatgaccac tagagggaag
      181 aagatacctg tggagctaat ggtccaagat gtcgggagcc gcgatcgctg ccaaaaaggt
      241 aagctgacga tagctgacga gattcgatgc ccgacgagca aatgagcgcc acggctgttc
      301 gcgtaagctc gaaacgacgg agattcgata gagccagagt cgagtttaaa cgattcggga
      361 tcttaaggat aaatttctgg taaggaggac acgtatggaa gtgggcaagt tggggaagcc
      421 gtatccgttg ctgaatctgg catatgtggg agtataagac gcgcagcgtc gcatcaggca
      481 tttttttctg cgccaatgca cctagaatcg gttaccaatg cttaatcagt gaggcaccta
      541 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
      601 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
      661 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
      721 gtggtcctgc aactttatcc gcctccatcc agtctatcaa ctgttgccgg gaagctagag
      781 taagtagttc gccagttaat agtttacgca aggttgttgc cattgctaca ggcatcgtgg
      841 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
      901 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccaatcgttg
      961 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     1021 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgaatactca accaagtcat
     1081 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     1141 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaagcgttct tcggggcgaa
     1201 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgcgcaccca
     1261 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     1321 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atgcgaaagc
     1381 ctcctgctga tcctgtctct tgatagatgt ttgcaaaagc taggactcga ttatacgttg
     1441 accgatcaga ctatcaatat ctaggtttaa acagatcggt acgctgccgt gaccaggctt
     1501 gagtacaata tctgacgatt ggacttcctg ggtcccaagc tggattccga ctggctgact
     1561 gccaagcaac gatatggatc gtcgagactc gcactgagag gatcgttctc ggtacaccag
     1621 gcgcgccccg atttcgatat tcctcggatc tcggatagat cgggattgag gatgtaggtt
     1681 gaacttctct cgggtgatcc cgatctcgga tattcgggat tggagtaata cctggcgata
     1741 ggcgctgcca agcaatagcc aaggcgcaag gccggccaag agacttgcga tagttcgttc
     1801 gagaggtagc ctgcaagatg gatcggacga aaggtttgaa gattttccga cgagggcctt
     1861 ggagagttcg gaaatttaaa tgcaactatg gatgaacgaa atagacagat cgctgagata
     1921 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     1981 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     2041 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     2101 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     2161 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     2221 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     2281 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     2341 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     2401 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     2461 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     2521 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     2581 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     2641 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     2701 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     2761 cacatgattt aaatgaaact cgtaatcgga gaacgggtgc aatagcaagg accagatcga
     2821 acttgactga cgcaattctg agcgtagtcg aaacccagac aagtcgatct cgccaagtag
     2881 ctgacttgac tggttaatta agtttcgata gcccaaggta accaatgaga ttagggaggg
     2941 aagaaagttt gatgaactac ttctgacccc tagtggtgtc cagaaaagac cattaaagga
     3001 atgtcttgat cgaaatcgag agatcgcctg caggatagct gacgacctta aggataaatt
     3061 tctggtaagg aggacacgta tggaagtggg caagttgggg aagccgtatc cgttgctgaa
     3121 tctggcatat gtgggagtat aagacgcgca gcgtcgcatc aggcattttt ttctgcgcca
     3181 atgccggtag atagcaatcc ggcccgaggg gcacaaaaaa cccctcaaga cccgtttaga
     3241 ggccccaagg ggttatgcta cgataccgat agagatgggc caataccaca tttgtagagg
     3301 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
     3361 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     3421 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     3481 tcatcaatgt atcttatcat gtctgtgagc tgaaggtacg ctgtatctca gtcagtcaag
     3541 ctagcttagg tagaatctaa gccgagaagt ggattgggta taggtttccc cgactgaaag
     3601 taaaggtttt cgtcaatctc cattggctgt tcctcatcca gttgggaata tttattctca
     3661 gttaaagact tactaaggtt ttttatgatg agattaatga tgtagtccag gttccaaatg
     3721 ctaaccttga aggttcgcat gataattagc agtatttctg cgatcgtgac ctgaaaatcc
     3781 accaagtgga acatggtggc ggccgctggc gggtgtgtcg agtggatggt aggatcgaca
     3841 aagatctgat ctgacggttc actaaaccag ctctgcttat atagacctcc caccgtacac
     3901 gcctaccgcc catttgcgtc aatggggcgg agttgttacg acattttgga aagtcccgtt
     3961 gattttggtg ccaaaacaaa ctcccattga cgtcaatggg gtggagactt ggaaatcccc
     4021 gtgagtcaaa ccgctatcca cgcccattga tgtactgcca aaaccgcatc agcatggtaa
     4081 tagcgatgac taatacgtag atgtactgcc aagtaggaaa gtcccataag gtcatgtact
     4141 gggcataatg ccaggcgggc catttaccgt cattgacgtc aatagggggc gtacttggca
     4201 tatgatacac ttgatgtact gccaagtggg cagtttaccg taaatactcc acccattgac
     4261 gtcaatggaa agtccctatt ggcgttacta tgggaacata cgtcattatt gacgtcaatg
     4321 ggcgggggtc gttgggcggt cagccaggcg ggccatttac cgtaagttat gtaacgcgga
     4381 actccatata tgggctatga actaatgacc ccgtaattga ttactagatc tggc
//
