LOCUS       sequence-300779-        1985 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial Expression plasmid for SARS-CoV-2 nsp7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1985)
  TITLE     SARS-CoV-2 protein E. coli expression constructs, tagged, for 
            protein expression and/or purification
REFERENCE   2  (bases 1 to 1985)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4046919
FEATURES             Location/Qualifiers
     source          1..1985
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     20..39
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        20..38
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    39..63
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             78..100
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             354..374
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             375..398
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     rep_origin      519..1107
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1008..1027
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             1213..1872
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1279..1298)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1943..1977
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 gatctcgatc ccgcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat
       61 tcccctctag aaataatttt gtttaacttt aagaaggaga ctcttctatg aaaatgtcag
      121 atgtaaagtg cacatcagta gtcttactct cagttttgca acaactcaga gtagaatcat
      181 catctaaatt gtgggctcaa tgtgtccagt tacacaatga cattctctta gctaaagata
      241 ctactgaagc ctttgaaaaa atggtttcac tactttctgt tttgctttcc atgcagggtg
      301 ctgtagacat aaacaagctt tgtgaagaaa tgctggacaa cagggcaacc ttagagaacc
      361 tgtactttca aagctggagc catccgcagt ttgaaaaata accggcttat cggtcagttt
      421 cacctgattt acgtaaaaac ccgcttcggc gggtttttgc ttttggaggg gcagaaagat
      481 gaatgactgt ccacgacgct atacccaaaa gaaaaagctt gagatccttt ttttctgcgc
      541 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
      601 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
      661 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
      721 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
      781 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
      841 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
      901 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
      961 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     1021 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     1081 tcgtcagggg ggcggagcct atggaaattg atcgggcacg taagaggttc caactttcac
     1141 cataatgaaa taagatcact accgggcgta ttttttgagt tatcgagatt ttcaggagct
     1201 aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg
     1261 catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc
     1321 gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat
     1381 ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt tcgtatggca
     1441 atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac cgttttccat
     1501 gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt ccggcagttt
     1561 ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa
     1621 gggtttattg agaatatgtt tttcgtttca gccaatccct gggtgagttt caccagtttt
     1681 gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat gggcaaatat
     1741 tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtttgt
     1801 gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga tgagtggcag
     1861 ggcggggcgt aatttgatat cgagctcgct tggactcctg ttgatagatc cagtaatgac
     1921 ctcagaactc catctggatt tgttcagaac gctcggttgc cgccgggcgt tttttattgg
     1981 tcatc
//
