LOCUS       sequence-300787-        2108 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Single-plasmid multi-gene expression system. Each genes under the 
            control of a T7 and the lacO promoter sequences..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Kufs JE, Hoefgen S, Rautschek J, Bissell AU, Graf C, Fiedler J, 
            Braga D, Regestein L, Rosenbaum MA, Thiele J, Valiante V
  TITLE     Rational design of flavonoid production routes using combinatorial 
            and precursor-directed biosynthesis.
  JOURNAL   ACS Synth Biol. 2020 Jun 11. doi: 10.1021/acssynbio.0c00172.
  PUBMED    32525654
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2020 Jun 11. doi: 10.1021/acssynbio.0c00172."
COMMENT     Sequence Label: pMGE-T7
FEATURES             Location/Qualifiers
     source          1..2108
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    24..40
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      129..175
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        229..247
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    252..268
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(421..440)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             432..1247
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPHLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(505..524)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      complement(1435..2023)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1515..1534)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 taatacgact cactataggg gaattgtgag cggataacaa tttgcgcaag aaggagatag
       61 atatctgcac tgagtgaagc aaccccgcgg ggcctcttcg ggggtctcgc ggggtttttt
      121 gctgaaagca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatcctcg
      181 cgtaacccct tggggcctct aaacgggtct tgaggggttt tttgaaacta atacgactca
      241 ctatagggga attgtgagcg gataacaatt taaattcctt aattaaggat cccaactcag
      301 caaaagttcg atttattcaa caaagccacg ttgtgtctca aaatctctga tgttacattg
      361 cacaagataa aaatatatca tcatgaacaa taaaactgtc tgcttacata aacagtaata
      421 caaggggtgt tatgagccat attcaacggg aaacgtcttg ctcgaggccg catttaaatt
      481 ccaacatgga tgctgattta tatgggtata aatgggctcg cgataatgtc gggcaatcag
      541 gtgcgacaat ctatcgattg tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg
      601 gcaaaggtag cgttgccaat gatgttacag atgagatggt ccgtttaaac tggctgacgg
      661 aatttatgcc tcttccgacc atcaagcatt ttatccgtac tcctgatgat gcatggttac
      721 tcaccactgc gatccccggg aaaacagcat tccaggtatt agaagaatat cctgattcag
      781 gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg attcctgttt
      841 gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga
      901 ataacggttt ggttgatgcg agtgattttg atgacgagcg taatggctgg cctgttgaac
      961 aagtctggaa agaaatgcat aagcttttgc cattctcacc ggattcagtc gtcactcatg
     1021 gtgatttctc acttgataac cttatttttg acgaggggaa attaataggt tgtattgatg
     1081 ttggacgagt cggaatcgca gaccgatacc aggatcttgc catcctatgg aactgcctcg
     1141 gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt gataatcctg
     1201 atatgaataa attgcagttt catttgatgc tcgatgagtt tttctaatca gaattggtta
     1261 attggttgta acactggcag agcattacgc tgacttgacg cggcgagcgg tatcagctca
     1321 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     1381 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     1441 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     1501 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     1561 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     1621 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     1681 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     1741 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     1801 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     1861 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     1921 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     1981 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     2041 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggttctaga
     2101 gtttaaac
//
