LOCUS       sequence-300790-        4066 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Template to synthesize riboprobes for in situ hybridization (ISH) 
            for Olfr1338.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4066)
  AUTHORS   Zapiec B, Mombaerts P
  TITLE     The Zonal Organization of Odorant Receptor Gene Choice in the Main 
            Olfactory Epithelium of the Mouse.
  JOURNAL   Cell Rep. 2020 Mar 24;30(12):4220-4234.e5. doi: 
            10.1016/j.celrep.2020.02.110.
  PUBMED    32209480
REFERENCE   2  (bases 1 to 4066)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2020.02"; journalName: "Cell Rep"; date: 
            "2020-03-24- 24"; volume: "30"; issue: "12"; pages: "4220-4234.e5"
COMMENT     Sequence Label: pISH-Olfr1338
FEATURES             Location/Qualifiers
     source          1..4066
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     124..143
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     377..394
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    511..532
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(547..552,553..570,571..577)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    585..601
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     590..612
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     609..625
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     609..625
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        643..661
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     643..660
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      1806..1815
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1810..1819
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(1849..1868)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1850..1868)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1875..1892)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1875..1891)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1884..1906)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2032..2487
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2119..2138)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2329..2350
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2565..2669
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2670..2738,2739..3530)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2888..2907)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(3701..4066,1..223)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
       61 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
      121 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
      181 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
      241 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
      301 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
      361 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
      421 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
      481 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
      541 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
      601 atttcacaca ggaaacagct atgaccatga ttacgccaag ctatttaggt gacactatag
      661 aatactcaag ctatgcatcc aacgcgttgg gagctctccc atatggtcga cctgcaggcg
      721 gccgcgaatt cactagtgat tttccctttc tgcctctatc gatgtcatgt gtcagatgat
      781 acatagaata atggattctc ttacaggctc caccatgtgg atgtttccaa ggcagaacca
      841 aagttgggtt tcagagttca tcctgattgg cttctccagt gatcccacaa ccaacagcat
      901 cctcttcatt gtcttccttc tcatctacct gaactcagtc ctgggcaacg gactcatcat
      961 catgctggtc tgcctggaca cacagctgca cactcctatg tactttttcc tctgtaccct
     1021 ctccctgttg gatatgagct atgtcactac caccatgccc cagatgttgg tgcatcttct
     1081 tgctcactct cagaccatct cctttgctgg ctgctggctg cagatgtata tgttcagtgc
     1141 cctgggtata gctgagagca tcctctttgt tgtgatggct tatgaccggt atgtggcaat
     1201 ttgctatcca ctgcgctata ctgtcatcct caactggggc ctgtgcatac gattggcagc
     1261 agggacttgg atctgtggtt ccttttcctc tttgttgcat actttcttca ccatgagtct
     1321 gccatattgt gggcccaaaa gggttgacca ctatttctgt gaaggtcctt cagtgcgtag
     1381 tctggcttgc atggataccc acctcattga gatggtggac ttggtgttga gtgtttttgt
     1441 ggttgttact ccaatttccc tcattgtggc ttcctacatt cacattacca aggcaattct
     1501 caagatcaag tccacccagg cccgctgtaa agctttctct acctgtgcct cccacctgac
     1561 tgtggtcaca tttttctata ttccagccat ttacatctac attaggccaa actccagcta
     1621 ctcccctgag agagacaagc agatctcact cttttacaat gttttcacag ccttgctcaa
     1681 ccctgtggtc tatagtctga gaaacaagga cattaagagg gcattctcaa ggtaatggga
     1741 cttggtagga tggactggtg aaccagcatc ctgggaagct ggagtattca aatcgaattc
     1801 ccgcggccgc catggcggcc gggagcatgc gacgtcgggc ccaattcgcc ctatagtgag
     1861 tcgtattaca attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     1921 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     1981 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gacgcgccct
     2041 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     2101 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     2161 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     2221 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     2281 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     2341 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     2401 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     2461 ttaacaaaat attaacgctt acaatttcct gatgcggtat tttctcctta cgcatctgtg
     2521 cggtatttca caccgcatca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     2581 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     2641 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     2701 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     2761 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     2821 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     2881 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     2941 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     3001 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     3061 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     3121 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     3181 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     3241 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     3301 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     3361 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     3421 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     3481 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     3541 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     3601 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     3661 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     3721 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     3781 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     3841 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     3901 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     3961 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     4021 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacac
//
