LOCUS       sequence-30091-e         647 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 647)
  AUTHORS   Potter CJ, Tasic B, Russler EV, Liang L, Luo L
  TITLE     The Q system: a repressible binary system for transgene expression, 
            lineage tracing, and mosaic analysis.
  JOURNAL   Cell. 2010 Apr 30. 141(3):536-48.
  PUBMED    20434990
REFERENCE   2  (bases 1 to 647)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2010 
            Apr 30. 141(3):536-48."
COMMENT     Sequence Label: pCMV-GAL4
FEATURES             Location/Qualifiers
     source          1..647
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(30..59)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(88..432)
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
ORIGIN
        1 tgatggtgat gatgaccggt atgcatattc agatcctctt ctgagatgag tttttgttcg
       61 aagggccctc tagactcgag cggccgctta ctcttttttt gggtttggtg gggtatcttc
      121 atcatcgaat agatagttat atacatcatc cattgtagtg gtattaaaca tccctgtagt
      181 gattccaaac gcgttatacg cagtttggtc cgtccaacca ggtgacagtg gttttgaatt
      241 attaccatca tcaattttac tagccgtgat ttcattattc atgaagttat catgaacgtt
      301 agaggaggca attggttgtg aaagcgcttg agaatttgtt tgagttgtta tgaggttcgg
      361 accgttgcta ctgttagtga aagtgaagga caatgagcta tcagcaatat tcccactttg
      421 attaaaattg gcgccaccaa acaaagcaga cggggtcagt ggcactaatg attgcagctg
      481 ttgctgttgc cctagaaaag gcgtgactga gcgatgcgaa ggtgtgcttc ttggtattgt
      541 cactggagag ttacgagagg gtggacggtt agataacagc ttgactagat cactgaaact
      601 tgctcctgat ttcaatggca caggtgaagg ccctactgag ccaggag
//
