LOCUS       sequence-301022-        2082 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For use in RBP tethering screen.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Luo EC, Nathanson JL, Tan FE, Schwartz JL, Schmok JC, Shankar A, 
            Markmiller S, Yee BA, Sathe S, Pratt GA, Scaletta DB, Ha Y, Hill DE,
            Aigner S, Yeo GW
  TITLE     Large-scale tethered function assays identify factors that regulate 
            mRNA stability and translation.
  JOURNAL   Nat Struct Mol Biol. 2020 Oct;27(10):989-1000. doi: 
            10.1038/s41594-020-0477-6. Epub 2020 Aug 17.
  PUBMED    32807991
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41594-020-0477-6"; journalName: "Nat Struct Mol Biol"; 
            date: "2020-10"; volume: "27"; issue: "10"; pages: "989-1000"
COMMENT     Sequence Label: FZD8
FEATURES             Location/Qualifiers
     source          1..2082
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             924..941
                     /codon_start=1
                     /product="recognition and cleavage site for Genenase(TM) I,
                     a subtilisin BPN' variant engineered for increased 
                     substrate specificity (Carter and Wells, 1987)"
                     /label=Genenase(TM) I site
                     /translation="PGAAHY"
ORIGIN
        1 atggagtggg gttacctgtt ggaagtgacc tcgctgctgg ccgccttggc gctgctgcag
       61 cgctctagcg gcgctgcggc cgcctcggcc aaggagctgg catgccaaga gatcaccgtg
      121 ccgctgtgta agggcatcgg ctacaactac acctacatgc ccaatcagtt caaccacgac
      181 acgcaagacg aggcgggcct ggaggtgcac cagttctggc cgctggtgga gatccagtgc
      241 tcgcccgatc tcaagttctt cctgtgcagc atgtacacgc ccatctgcct agaggactac
      301 aagaagccgc tgccgccctg ccgctcggtg tgcgagcgcg ccaaggccgg ctgcgcgccg
      361 ctcatgcgcc agtacggctt cgcctggccc gaccgcatgc gctgcgaccg gctgcccgag
      421 caaggcaacc ctgacacgct gtgcatggac tacaaccgca ccgacctaac caccgccgcg
      481 cccagcccgc cgcgccgcct gccgccgccg ccgcccggcg agcagccgcc ttcgggcagc
      541 ggccacggcc gcccgccggg ggccaggccc ccgcaccgcg gcggcggcag gggcggtggc
      601 ggcggggacg cggcggcgcc cccagctcgc ggcggcggcg gtggcgggaa ggcgcggccc
      661 cctggcggcg gcgcggctcc ctgcgagccc gggtgccagt gccgcgcgcc tatggtgagc
      721 gtgtccagcg agcgccaccc gctctacaac cgcgtcaaga caggccagat cgctaactgc
      781 gcgctgccct gccacaaccc ctttttcagc caggacgagc gcgccttcac cgtcttctgg
      841 atcggcctgt ggtcggtgct ctgcttcgtg tccaccttcg ccaccgtctc caccttcctt
      901 atcgacatgg agcgcttcaa gtacccggag cggcccatta tcttcctctc ggcctgctac
      961 ctcttcgtgt cggtgggcta cctagtgcgc ctggtggcgg gccacgagaa ggtggcgtgc
     1021 agcggtggcg cgccgggcgc ggggggcgct gggggcgcgg gcggcgcggc ggcgggcgcg
     1081 ggcgcggcgg gcgcgggcgc gggcggcccg ggcgggcgcg gcgagtacga ggagctgggc
     1141 gcggtggagc agcacgtgcg ctacgagacc accggccccg cgctgtgcac cgtggtcttc
     1201 ttgctggtct acttcttcgg catggccagc tccatctggt gggtgatctt gtcgctcaca
     1261 tggttcctgg cggccggtat gaagtggggc aacgaagcca tcgccggcta ctcgcagtac
     1321 ttccacctgg ccgcgtggct tgtgcccagc gtcaagtcca tcgcggtgct ggcgctcagc
     1381 tcggtggacg gcgacccggt ggcgggcatc tgctacgtgg gcaaccagag cctggacaac
     1441 ctgcgcggct tcgtgctggc gccgctggtc atctacctct tcatcggcac catgttcctg
     1501 ctggccggct tcgtgtccct cttccgcatc cgctcggtca tcaagcaaca ggacggcccc
     1561 accaagacgc acaagctgga gaagctgatg atccgcctgg gcctgttcac cgtgctctac
     1621 accgtgcccg ccgcggtggt ggtcgcctgc ctcttctacg agcagcacaa ccgcccgcgc
     1681 tgggaggcca cgcacaactg cccgtgcctg cgggacctgc agcccgacca ggcacgcagg
     1741 cccgactacg ccgtcttcat gctcaagtac ttcatgtgcc tagtggtggg catcacctcg
     1801 ggcgtgtggg tctggtccgg caagacgctg gagtcctggc gctccctgtg cacccgctgc
     1861 tgctgggcca gcaagggcgc cgcggtgggc gggggcgcgg gcgccacggc cgcggggggt
     1921 ggcggcgggc cggggggcgg cggcggcggg ggacccggcg gcggcggggg gccgggcggc
     1981 ggcgggggct ccctctacag cgacgtcagc actggcctga cgtggcggtc gggcacggcg
     2041 agctccgtgt cttatccaaa gcagatgcca ttgtcccagg tc
//
