LOCUS       sequence-30109-e         671 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Potter CJ, Tasic B, Russler EV, Liang L, Luo L
  TITLE     The Q system: a repressible binary system for transgene expression, 
            lineage tracing, and mosaic analysis.
  JOURNAL   Cell. 2010 Apr 30. 141(3):536-48.
  PUBMED    20434990
REFERENCE   2  (bases 1 to 671)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2010 
            Apr 30. 141(3):536-48."
COMMENT     Sequence Label: pQUAST-mtdT-3XHA
FEATURES             Location/Qualifiers
     source          1..671
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    4..138
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(28..47)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     82..101
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(410..430)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          560..625
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
ORIGIN
        1 gtggatccag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag
       61 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
      121 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
      181 gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat
      241 gatctctagt caaggcacta tacatcaaat atttttccat aattttcttg tatagcagtg
      301 cagctttttc ctttgtggtg taaatagcaa agcaagcaag agttctatta ctaaacacag
      361 catgactcaa aaaacttagc aattctgaag gaaagtcctt ggggtcttct acctttctct
      421 tcttttttgg aggagtagaa tgttgagagt cagcagtagc ctcatcatca ctagatggca
      481 tttcttctga gcaaaacagg ttttcctcat taaaggcatt ccaccactgc tcccattcat
      541 cagttccata ggttggaatc taaaatacac aaacaattag aatcagtagt ttaacacatt
      601 atacacttaa aaattttata tttaccttag agctttaaat ctctgtaggt agtttgtcca
      661 attatgtcac a
//
