LOCUS       sequence-30112-e         660 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Potter CJ, Tasic B, Russler EV, Liang L, Luo L
  TITLE     The Q system: a repressible binary system for transgene expression, 
            lineage tracing, and mosaic analysis.
  JOURNAL   Cell. 2010 Apr 30. 141(3):536-48.
  PUBMED    20434990
REFERENCE   2  (bases 1 to 660)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2010 
            Apr 30. 141(3):536-48."
COMMENT     Sequence Label: pUASTattB-mtdT-3XHA
FEATURES             Location/Qualifiers
     source          1..660
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(275..295)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          425..490
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     primer_bind     581..597
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     582..598
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(622..648)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt
       61 ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatct ctagtcaagg
      121 cactatacat caaatatttt tccataattt tcttgtatag cagtgcagct ttttcctttg
      181 tggtgtaaat agcaaagcaa gcaagagttc tattactaaa cacagcatga ctcaaaaaac
      241 ttagcaattc tgaaggaaag tccttggggt cttctacctt tctcttcttt tttggaggag
      301 tagaatgttg agagtcagca gtagcctcat catcactaga tggcatttct tctgagcaaa
      361 acaggttttc ctcattaaag gcattccacc actgctccca ttcatcagtt ccataggttg
      421 gaatctaaaa tacacaaaca attagaatca gtagtttaac acattataca cttaaaaatt
      481 ttatatttac cttagagctt taaatctctg taggtagttt gtccaattat gtcacaccac
      541 agaagtaagg ttccttcaca aagatcctct agaggtaccc tcgaggtcga cggtatcgat
      601 aagcttgata tcgaattctt aagcgtaatc tggaacgtca tatggatagg atcctgcata
//
