LOCUS       sequence-30114-e         602 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 602)
  AUTHORS   Potter CJ, Tasic B, Russler EV, Liang L, Luo L
  TITLE     The Q system: a repressible binary system for transgene expression, 
            lineage tracing, and mosaic analysis.
  JOURNAL   Cell. 2010 Apr 30. 141(3):536-48.
  PUBMED    20434990
REFERENCE   2  (bases 1 to 602)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2010 
            Apr 30. 141(3):536-48."
COMMENT     Sequence Label: pQUAST-nucLacZ
FEATURES             Location/Qualifiers
     source          1..602
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     154..176
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             complement(191..211)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        complement(316..554)
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     complement(348..369)
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
ORIGIN
        1 gcttctggtg ccggaaccag gcaaagcgcc attcgccatt caggctgcgc aactgttggg
       61 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg
      121 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg
      181 gatcgtcctc caccttgcgc ttcttcttgg gtggggtggg atctcccatg gtggtctagg
      241 taccctcgag ccgcggccgc agatctgtta acgaattgat cccccgatct gttaacgaat
      301 tcccaattcc ctattcagag ttctcttctt gtattcaata attacttctt ggcagatttc
      361 agtagttgca gttgatttac ttggttgctg gttactttta attgattcac tttaacttgc
      421 actttactgc agattgttta gcttgttcag ctgcgcttgt ttatttgctt agctttcgct
      481 tagcgacgtg ttcactttgc ttgtttgaat tgaattgtcg ctccgtagac gaagcgcctc
      541 tatttatact ccggcgctcg ctagagtctc cgctcgggct gcaggaattc gatatcaagc
      601 tt
//
