LOCUS       sequence-30127-e         576 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 576)
  AUTHORS   Potter CJ, Tasic B, Russler EV, Liang L, Luo L
  TITLE     The Q system: a repressible binary system for transgene expression, 
            lineage tracing, and mosaic analysis.
  JOURNAL   Cell. 2010 Apr 30. 141(3):536-48.
  PUBMED    20434990
REFERENCE   2  (bases 1 to 576)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2010 
            Apr 30. 141(3):536-48."
COMMENT     Sequence Label: pQUAST-shibire (ts1)
FEATURES             Location/Qualifiers
     source          1..576
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        206..444
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     391..412
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     primer_bind     508..524
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     508..524
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     regulatory      534..543
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cgagtacgca aagcttggct gcatccnacg cgttgggagc tctccggatc caagcttgga
       61 tccgggtaat cgcttatcct cggataaaca attatcctca cgggtaatcg cttatccgct
      121 cgggtaatcg cttatcctcg ggtaatcgct tatccttaag cttgatatcg aattcctgca
      181 gcccgagcgg agactctagc gagcgccgga gtataaatag aggcgcttcg tctacggagc
      241 gacaattcaa ttcaaacaag caaagtgaac acgtcgctaa gcgaaagcta agcaaataaa
      301 caagcgcagc tgaacaagct aaacaatctg cagtaaagtg caagttaaag tgaatcaatt
      361 aaaagtaacc agcaaccaag taaatcaact gcaactactg aaatctgcca agaagtaatt
      421 attgaataca agaagagaac tctgaatagg gaattgggaa ttcgttaaca gatcggggga
      481 tcaattcgtt aacagatctg cggccgctct agaactagtg gatccaagga gccgccgcca
      541 tggatagttt aattacaatt gttaacaagc tgcaag
//
