LOCUS       sequence-30128-e         712 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Potter CJ, Tasic B, Russler EV, Liang L, Luo L
  TITLE     The Q system: a repressible binary system for transgene expression, 
            lineage tracing, and mosaic analysis.
  JOURNAL   Cell. 2010 Apr 30. 141(3):536-48.
  PUBMED    20434990
REFERENCE   2  (bases 1 to 712)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2010 
            Apr 30. 141(3):536-48."
COMMENT     Sequence Label: pQUAST-shibire (ts1)
FEATURES             Location/Qualifiers
     source          1..712
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    8..129
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(19..38)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     73..92
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(401..421)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          551..616
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
ORIGIN
        1 gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa
       61 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
      121 aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg
      181 gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta tgatctctag
      241 tcaaggcact atacatcaaa tatttttcca taattttctt gtatagcagt gcagcttttt
      301 cctttgtggt gtaaatagca aagcaagcaa gagttctatt actaaacaca gcatgactca
      361 aaaaacttag caattctgaa ggaaagtcct tggggtcttc tacctttctc ttcttttttg
      421 gaggagtaga atgttgagag tcagcagtag cctcatcatc actagatggc atttcttctg
      481 agcaaaacag gttttcctca ttaaaggcat tccaccactg ctcccattca tcagttccat
      541 aggttggaat ctaaaataca caaacaatta gaatcagtag tttaacacat tatacactta
      601 aaaattttat atttacctta gagctttaaa tctctgtagg tagtttgtcc aattatgtca
      661 caccacagaa gtaaggttcc ttcacaaaga tcctctagag gtaccctcga ag
//
