LOCUS       sequence-30129-e         683 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Potter CJ, Tasic B, Russler EV, Liang L, Luo L
  TITLE     The Q system: a repressible binary system for transgene expression, 
            lineage tracing, and mosaic analysis.
  JOURNAL   Cell. 2010 Apr 30. 141(3):536-48.
  PUBMED    20434990
REFERENCE   2  (bases 1 to 683)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2010 
            Apr 30. 141(3):536-48."
COMMENT     Sequence Label: pQUAST>stop>shibire (ts1)
FEATURES             Location/Qualifiers
     source          1..683
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        226..464
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     411..432
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     protein_bind    539..586
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
ORIGIN
        1 gttttatttt taataatttg cgagtacgca aagcttggct gcatccaacg cgttgggagc
       61 tctccggatc caagcttgga tccgggtaat cgcttatcct cggataaaca attatcctca
      121 cgggtaatcg cttatccgct cgggtaatcg cttatcctcg ggtaatcgct tatccttaag
      181 cttgatatcg aattcctgca gcccgagcgg agactctagc gagcgccgga gtataaatag
      241 aggcgcttcg tctacggagc gacaattcaa ttcaaacaag caaagtgaac acgtcgctaa
      301 gcgaaagcta agcaaataaa caagcgcagc tgaacaagct aaacaatctg cagtaaagtg
      361 caagttaaag tgaatcaatt aaaagtaacc agcaaccaag taaatcaact gcaactactg
      421 aaatctgcca agaagtaatt attgaataca agaagagaac tctgaatagg gaattgggaa
      481 ttcgttaaca gatcggggga tcaattcgtt aacagatctg agggtacccg gggatcttga
      541 agttcctatt ccgaagttcc tattctctag aaagtatagg aacttcagag cgcttttgaa
      601 gctagagaat tcgcgcacta gtgctagagg atctttgtga aggaacctta cttctgtggt
      661 gtgacataat tggacaaact aca
//
