LOCUS       sequence-30130-e         642 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Potter CJ, Tasic B, Russler EV, Liang L, Luo L
  TITLE     The Q system: a repressible binary system for transgene expression, 
            lineage tracing, and mosaic analysis.
  JOURNAL   Cell. 2010 Apr 30. 141(3):536-48.
  PUBMED    20434990
REFERENCE   2  (bases 1 to 642)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2010 
            Apr 30. 141(3):536-48."
COMMENT     Sequence Label: pQUAST>stop>shibire (ts1)
FEATURES             Location/Qualifiers
     source          1..642
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    7..128
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(18..37)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     72..91
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(400..420)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          550..615
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
ORIGIN
        1 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat
       61 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
      121 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg
      181 aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat gatctctagt
      241 caaggcacta tacatcaaat atttttccat aattttcttg tatagcagtg cagctttttc
      301 ctttgtggtg taaatagcaa agcaagcaag agttctatta ctaaacacag catgactcaa
      361 aaaacttagc aattctgaag gaaagtcctt ggggtcttct acctttctct tcttttttgg
      421 aggagtagaa tgttgagagt cagcagtagc ctcatcatca ctagatggca tttcttctga
      481 gcaaaacagg ttttcctcat taaaggcatt ccaccactgc tcccattcat cagttccata
      541 ggttggaatc taaaatacac aaacaattag aatcagtagt ttaacacatt atacacttaa
      601 aaattttata tttaccttag agctttaaat ctctgtaggt ag
//
