LOCUS       sequence-30136-e         915 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Potter CJ, Tasic B, Russler EV, Liang L, Luo L
  TITLE     The Q system: a repressible binary system for transgene expression, 
            lineage tracing, and mosaic analysis.
  JOURNAL   Cell. 2010 Apr 30. 141(3):536-48.
  PUBMED    20434990
REFERENCE   2  (bases 1 to 915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2010 
            Apr 30. 141(3):536-48."
COMMENT     Sequence Label: pUASTattB-QS
FEATURES             Location/Qualifiers
     source          1..915
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(122..360)
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     complement(154..175)
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     protein_bind    379..473
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    complement(506..539)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gcgacggagg agatatgtgc ggtagaggta gaggatcgcg ggcggcgaca tccccgacat
       61 ggcgtgccgg gatggtgttc atgttgggta cgatctgtta acgaattccc aattccctat
      121 tcagagttct cttcttgtat tcaataatta cttcttggca gatttcagta gttgcagttg
      181 atttacttgg ttgctggtta cttttaattg attcacttta acttgcactt tactgcagat
      241 tgtttagctt gttcagctgc gcttgtttat ttgcttagct ttcgcttagc gacgtgttca
      301 ctttgcttgt ttgaattgaa ttgtcgctcc gtagacgaag cgcctctatt tatactccgg
      361 cgctcgctag agtctccgct cggaggacag tactccgctc ggaggacagt actccgctcg
      421 gaggacagta ctccgctcgg aggacagtac tccgctcgga ggacagtact ccgacctgca
      481 ggcatgcaag cttggatccg ctagcataac ttcgtatagc atacattata cgaagttatg
      541 ctagcctagt tccagtgaaa tccaagcatt ttctaaatta aatgtattct tattattata
      601 gttgttattt ttgatatata taaacaacac tattatgccc accatttttt tgagatgcat
      661 ctacacaagg aacaaacact ggatgtcact ttcagttcaa attgtaacgc taatcactcc
      721 gaacaggtca caaaaaatta ccttaaaaag tcataatatt aaattagaat aaatatagct
      781 gtgagggaaa tatatacaaa tatattggag caaataaatt gtacatacaa atatttatta
      841 ctaatttcta ttgagacgaa atgaaccact cggaaccatt tgagcgaacc gaatcgcgcg
      901 gaactaacga cagtc
//
