LOCUS       sequence-30137-e         577 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Potter CJ, Tasic B, Russler EV, Liang L, Luo L
  TITLE     The Q system: a repressible binary system for transgene expression, 
            lineage tracing, and mosaic analysis.
  JOURNAL   Cell. 2010 Apr 30. 141(3):536-48.
  PUBMED    20434990
REFERENCE   2  (bases 1 to 577)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2010 
            Apr 30. 141(3):536-48."
COMMENT     Sequence Label: pUASTattB-QS
FEATURES             Location/Qualifiers
     source          1..577
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(4..23)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5..23)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(30..47)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(30..46)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(39..61)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      188..568
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(275..294)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     485..506
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 tcgccctata gtgagtcgta ttacaattca ctggccgtcg ttttacaacg tcgtgactgg
       61 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
      121 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
      181 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
      241 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
      301 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
      361 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
      421 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
      481 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
      541 gatttataag ggattttgcc gatttcggcc tattggt
//
