LOCUS       sequence-30144-e         788 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Potter CJ, Tasic B, Russler EV, Liang L, Luo L
  TITLE     The Q system: a repressible binary system for transgene expression, 
            lineage tracing, and mosaic analysis.
  JOURNAL   Cell. 2010 Apr 30. 141(3):536-48.
  PUBMED    20434990
REFERENCE   2  (bases 1 to 788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2010 
            Apr 30. 141(3):536-48."
COMMENT     Sequence Label: pUAST>stop>mCD8-GFP
FEATURES             Location/Qualifiers
     source          1..788
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          97..162
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             292..312
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    584..718
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(621..640)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     675..694
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(745..763)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
ORIGIN
        1 agactatcaa taatctagag gatctttgtg aaggaacctt acttctgtgg tgtgacataa
       61 ttggacaaac tacctacaga gatttaaagc tctaaggtaa atataaaatt tttaagtgta
      121 taatgtgtta aactactgat tctaattgtt tgtgtatttt agattccaac ctatggaact
      181 gatgaatggg agcagtggtg gaatgccttt aatgaggaaa acctgttttg ctcagaagaa
      241 atgccatcta gtgatgatga ggctactgct gactctcaac attctactcc tccaaaaaag
      301 aagagaaagg tagaagaccc caaggacttt ccttcagaat tgctaagttt tttgagtcat
      361 gctgtgttta gtaatagaac tcttgcttgc tttgctattt acaccacaaa ggaaaaagct
      421 gcactgctat acaagaaaat tatggaaaaa tatttgatgt atagtgcctt gactagagat
      481 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct
      541 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc
      601 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
      661 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcca
      721 ctagaaggcc taattctagt atgtatgtaa gttaataaaa cccatttttg cggaaagtag
      781 ataaaaaa
//
