LOCUS       sequence-30145-e         668 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Potter CJ, Tasic B, Russler EV, Liang L, Luo L
  TITLE     The Q system: a repressible binary system for transgene expression, 
            lineage tracing, and mosaic analysis.
  JOURNAL   Cell. 2010 Apr 30. 141(3):536-48.
  PUBMED    20434990
REFERENCE   2  (bases 1 to 668)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2010 
            Apr 30. 141(3):536-48."
COMMENT     Sequence Label: pUAST>stop>mCD8-GFP
FEATURES             Location/Qualifiers
     source          1..668
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    108..202
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        221..459
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     406..427
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     protein_bind    510..557
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
ORIGIN
        1 gacgaattat ttttaagttt tatttttaat aatttgcgag tacgcaaagc ttggctgcat
       61 ccaacgcgtt gggagctctc cggatccaag cttgcatgcc tgcaggtcgg agtactgtcc
      121 tccgagcgga gtactgtcct ccgagcggag tactgtcctc cgagcggagt actgtcctcc
      181 gagcggagta ctgtcctccg agcggagact ctagcgagcg ccggagtata aatagaggcg
      241 cttcgtctac ggagcgacaa ttcaattcaa acaagcaaag tgaacacgtc gctaagcgaa
      301 agctaagcaa ataaacaagc gcagctgaac aagctaaaca atctgcagta aagtgcaagt
      361 taaagtgaat caattaaaag taaccagcaa ccaagtaaat caactgcaac tactgaaatc
      421 tgccaagaag taattattga atacaagaag agaactctga atagggaatt gggaattcgt
      481 taacagatct gagggtaccc ggggatcttg aagttcctat tccgaagttc ctattctcta
      541 gaaagtatag gaacttcaga gcgcttttga agctagagaa ttcgcgcact agtgctagag
      601 gatctttgtg aaggaacctt acttctgtgg tgtgacataa ttggacaaac tacctacaga
      661 gatttaaa
//
