LOCUS       sequence-30148-e         631 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 631)
  AUTHORS   Lim HN, van Oudenaarden A
  TITLE     A multistep epigenetic switch enables the stable inheritance of DNA 
            methylation states.
  JOURNAL   Nat Genet. 2007 Feb . 39(2):269-75.
  PUBMED    17220888
REFERENCE   2  (bases 1 to 631)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2007 Feb . 39(2):269-75."
COMMENT     Sequence Label: pHL32 (in cHNL135)
FEATURES             Location/Qualifiers
     source          1..631
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             32..64
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     primer_bind     complement(104..132)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatataca tatggctagc atgactggtg gacagcaaat
       61 gggtcgcgga tccatgcgta aaggagaaga acttttcact ggagttgtcc caattcttgt
      121 tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga
      181 tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc
      241 atggccaaca cttgtcacta ctttcggtta tggtgttcaa tgctttgcga gatacccaga
      301 tcatatgaaa cagcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaaag
      361 aactatattt ttcaaagatg acgggaacta caagacacgt gctgaagtca agtttgaagg
      421 tgataccctt gttaatagaa tcgagttaaa aggtattgat tttaaagaag atggaaacat
      481 tcttggacac aaattggaat acaactataa ctcacacaat gtatacatca tggcagacaa
      541 acaaaagaat ggaatcaaag ttaacttcaa aattagacac aacattgaag atggaagcgt
      601 tcaactagca gaccattatc aacaaaatac t
//
