LOCUS       sequence-30151-e         776 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Sun Q, Fan W, Chen K, Ding X, Chen S, Zhong Q
  TITLE     Identification of Barkor as a mammalian autophagy-specific factor 
            for Beclin 1 and class III phosphatidylinositol 3-kinase.
  JOURNAL   Proc Natl Acad Sci U S A. 2008 Dec 9. 105(49):19211-6.
  PUBMED    19050071
REFERENCE   2  (bases 1 to 776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2008 Dec 9. 105(49):19211-6."
COMMENT     Sequence Label: pcDNA4-Beclin1 1-150
FEATURES             Location/Qualifiers
     source          1..776
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(13..78)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     complement(646..670)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    680..698
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    701..719
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        complement(721..772)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(750..770)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 agactcgagt cacttgtcat cgtcatcctt gtagtcgatg tcatgatctt tataatcacc
       61 gtcatggtct ttgtagtcgc ggccgccagt gtccagctgg tctaaaagag tatctgtgca
      121 ttcctcacag agtgggtgat ccacatctgt ctggcccgac atgatgtcaa aaaggtcccc
      181 agtgaccttc agtcttcggc tgaggttctc catggtgccg ccatcagatg cctccccaat
      241 cagagtgaag ctgttggcac tttctgtgga catcatcctg gctgggggga tgaatctgcg
      301 agagacacca tcctggcgag gagtttcaat aaatggctcc tctcctgagt tagtctcttc
      361 ctcctgggtc tctcctggtt tcgcctgggc tgtggtaagt aatggagctg tgagttcctg
      421 gatggtgaca cggtccagga tcttgaaact cgtgtccagt ttcaggggct ggctgcagcg
      481 ctggcacacg aagctcacct gcatggtgct gttgttggac gtcttagacc cttccatgga
      541 tccgagctcg gtaccaagct taagtttaaa cgctagagtc cggaggctgg atcggtcccg
      601 gtgtcttcta tggaggtcaa aacagcgtgg atggcgtctc caggcgatct gacggttcac
      661 taaacgagct cgtcgacgat ctctatcact gatagggaga tctctatcac tgatagggag
      721 agctctgctt atatagacct cccaccgtac acgcctaccg cccatttgcg tcaatg
//
