LOCUS       sequence-30152-e         777 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Sun Q, Fan W, Chen K, Ding X, Chen S, Zhong Q
  TITLE     Identification of Barkor as a mammalian autophagy-specific factor 
            for Beclin 1 and class III phosphatidylinositol 3-kinase.
  JOURNAL   Proc Natl Acad Sci U S A. 2008 Dec 9. 105(49):19211-6.
  PUBMED    19050071
REFERENCE   2  (bases 1 to 777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2008 Dec 9. 105(49):19211-6."
COMMENT     Sequence Label: pcDNA4-Beclin1 151-241
FEATURES             Location/Qualifiers
     source          1..777
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(14..79)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     complement(476..500)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    510..528
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    531..549
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        complement(551..754)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(580..600)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 tagactcgag tcacttgtca tcgtcatcct tgtagtcgat gtcatgatct ttataatcac
       61 cgtcatggtc tttgtagtcg cggccgccct ccagctgctg tcgtttaaat tcactgtatt
      121 ctctctgata ctgagcttcc tcctgatcca gtctctcagc ctcagcctgg accttctcga
      181 gattttctgc cactatcttg cggttctttt ccacgtcttc cagctcctgg atcagcctct
      241 cctcctctag tgccagctcc tttagctcca tctgtaactg ttcactgtca tcctcattca
      301 tttgctctaa gatctccaaa cagcgtttgt agttctgaca ctcattttca gtgacgttga
      361 gctgcatgga tccgagctcg gtaccaagct taagtttaaa cgctagagtc cggaggctgg
      421 atcggtcccg gtgtcttcta tggaggtcaa aacagcgtgg atggcgtctc caggcgatct
      481 gacggttcac taaacgagct cgtcgacgat ctctatcact gatagggaga tctctatcac
      541 tgatagggag agctctgctt atatagacct cccaccgtac acgcctaccg cccatttgcg
      601 tcaatggggc ggagttgtta cgacattttg gaaagtcccg ttgattttgg tgccaaaaca
      661 aactcccatt gacgtcaatg gggtggagac ttggaaatcc ccgtgagtca aaccgctatc
      721 cacgcccatt gatgtactgc caaaaccgca tcaccatggt aatagcgatg actaana
//
