LOCUS       sequence-301623-       11244 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express NSP14 gene along with HA and BASU.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11244)
  AUTHORS   Meyers JM, Ramanathan M, Shanderson RL, Beck A, Donohue L, Ferguson 
            I, Guo MG, Rao DS, Miao W, Reynolds D, Yang X, Zhao Y, Yang YY, 
            Blish C, Wang Y, Khavari PA
  TITLE     The proximal proteome of 17 SARS-CoV-2 proteins links to disrupted 
            antiviral signaling and host translation.
  JOURNAL   PLoS Pathog. 2021 Oct 1;17(10):e1009412. doi: 
            10.1371/journal.ppat.1009412.
  PUBMED    34597346
REFERENCE   2  (bases 1 to 11244)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Pathog."; date: "2021-10-1"; volume: "17(10):e1009412. doi"; pages: 
            " 10.1371/journal.ppat.1009412"
COMMENT     Sequence Label: SARS_COV2_nsp14_HA_BASU
FEATURES             Location/Qualifiers
     source          1..11244
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2337..3519
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2568..3510
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3467..3487
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3553..3579
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4366..4389
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     misc_feature    join(5994..6568,6569..6571,6572..6580)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6161..6178)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6388..6407
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6600..7199
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6600..6619)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7096..7116
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7213..7801
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7266..7286)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7684..7695)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8008..8641
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8769..8785)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8769..8785)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8782..8804)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8793..8809
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8817..8823,8824..8841,8842..8847))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8862..8883
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9000..9017)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9171..9759)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9251..9270)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9930..10721,10722..10790))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10553..10572
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10791..10895)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    10943..11077
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10980..10999)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11034..11053
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgagtaa ttcatacaaa aggactcgcc cctgccttgg
     2221 ggaatcccag ggaccgtcgt taaactccca ctaacgtaga acccagagat cgctgcgttc
     2281 ccgccccctc acccgcccgc tctcgtcatc actgaggtgg agaagagcat gcgtgaggct
     2341 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag
     2401 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg
     2461 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag
     2521 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt
     2581 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt
     2641 ccacgcccct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
     2701 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
     2761 cttgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
     2821 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
     2881 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
     2941 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
     3001 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
     3061 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
     3121 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
     3181 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
     3241 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
     3301 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     3361 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     3421 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     3481 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gatctatttc cggtgaattc
     3541 gccgccacca tgtacccata cgatgtgcct gactatgctg gcaagttgtc tgaatcagag
     3601 attaggtttg gactgaaaac ggaggtgatg ggccaacatc ttatctatca cgatgttttg
     3661 tccagcacac aaaagacagc ccatgaattg gcaaataaca atgcccccga gggtaccctc
     3721 gtggttgcag ataaacagac agccggtagg ggcgggatga gccgagtgtg gcattctcaa
     3781 gaaggaaacg gagtatggat gagtttgata ttgcgcccgg acattccgct ccaaaagacg
     3841 ccccaactta ccttgctggc agctgtagcg gtggttcagg gaatagaaga ggccgcgggc
     3901 atacagactg atataaagtg gccgaacgac atactcatta acgggaaaaa aacagtaggg
     3961 atacttaccg aaatgcaagc tgaggaggat cgagtgcgct ccgtgattat tggtattggc
     4021 attaatgtaa atcagcaacc caatgatttc cccgatgagc tcaaggacat tgcgacgagt
     4081 ctgtcccagg cagctgggga aaagatcgac agggcaggtg taatccagca tatcttgctt
     4141 tgtttcgaaa agcgataccg agactacatg acccacggtt tcacgcccat caagctcttg
     4201 tgggagtcat acgccctcgg tattggcacg aacatgaggg cgagaactct caacgggaca
     4261 ttttacggta aggcgttggg tattgacgac gaaggggtac ttttgttgga aactaatgaa
     4321 ggcattaaga agatatacag cgcggacatc agccttaggg ggagctggtc tcacccgcaa
     4381 tttgaaaaag gcgctgcggc tgctgaaaat gtaacgggct tgtttaaaga ctgtagtaaa
     4441 gtgatcacag gactccaccc cacacaagca cctacccacc tttccgtaga tacgaagttc
     4501 aaaacggaag gattgtgtgt ggatatacca gggataccaa aggatatgac gtaccgaagg
     4561 ctgatttcca tgatgggttt taagatgaat taccaagtta atggctaccc caacatgttc
     4621 atcaccaggg aggaggcaat tagacacgta agagcctgga taggcttcga tgttgaaggt
     4681 tgccatgcaa cccgggaagc cgtaggcaca aaccttccgt tgcagcttgg cttttccacg
     4741 ggcgtcaacc tcgttgccgt accgactggc tatgttgaca cgccgaacaa caccgatttc
     4801 tctcgcgtaa gtgctaagcc tcctccggga gatcaattca agcatcttat acctctcatg
     4861 tacaagggac tgccgtggaa tgtagttcgc attaagatcg tgcagatgct ctccgatact
     4921 ttgaaaaatc tgagcgacag ggtggtcttc gttctgtggg cacatggctt cgaacttact
     4981 tctatgaaat actttgtaaa aatcgggccc gagcggacat gttgtttgtg cgaccgacga
     5041 gctacttgtt ttagcacagc atctgacacc tacgcatgct ggcaccacag tataggcttc
     5101 gattacgtct acaatccctt tatgatagac gtacaacaat ggggctttac gggtaacttg
     5161 cagagtaatc acgatcttta ctgccaagtt catgggaacg cacacgtggc ctcctgcgat
     5221 gcgataatga cgaggtgctt ggccgtgcac gagtgctttg ttaagcgggt cgattggact
     5281 atagagtatc ccataatcgg tgacgaactt aaaattaatg ctgcatgcag gaaagtgcag
     5341 cacatggtag taaaagcagc gctcctcgcg gataagtttc ctgtgctcca cgatatcggc
     5401 aaccctaagg ccattaaatg cgtcccccaa gctgatgtag agtggaaatt ctatgacgct
     5461 cagccatgta gtgacaaagc atacaagatt gaagaattgt tttattcata tgcgacacac
     5521 tccgataagt tcactgacgg agtttgtctc ttttggaact gtaatgttga taggtatccg
     5581 gcgaatagca tagtgtgtcg atttgacacg cgcgttctct ctaatttgaa cctccctggc
     5641 tgcgacgggg ggtctttgta cgtaaacaag catgcatttc ataccccagc ttttgacaaa
     5701 tccgctttcg taaatctgaa acaacttccc ttcttctatt attcagatag cccttgcgaa
     5761 tctcacggta agcaagtggt tagtgacatt gactatgtac cacttaaaag tgcaacgtgc
     5821 attacacgat gcaatttggg gggcgcggtt tgccgacacc atgccaacga atacagattg
     5881 tacctggatg catataacat gatgataagt gcagggttta gtctttgggt gtataagcag
     5941 ttcgatacgt ataatctctg gaacacattt acccgattgc agtagggatc ccgcccctct
     6001 ccctcccccc cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt
     6061 gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct
     6121 ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa
     6181 ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg
     6241 tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc
     6301 caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg
     6361 agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg
     6421 aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc
     6481 tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg
     6541 gttttccttt gaaaaacacg atgataagct tgccacaacc cacaaggaga cgaccttcca
     6601 tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc cgggccgtac
     6661 gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gacccggacc
     6721 gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca
     6781 tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga
     6841 gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt
     6901 cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc
     6961 ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca
     7021 gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg
     7081 agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg
     7141 acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctaga
     7201 cgcgtctgga acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     7261 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     7321 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     7381 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     7441 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     7501 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     7561 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt
     7621 ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     7681 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     7741 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     7801 cctggaatta attctgcagt cgagacctag aaaaacatgg agcaatcaca agtagcaata
     7861 cagcagctac caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt
     7921 ttccagtcac acctcaggta cctttaagac caatgactta caaggcagct gtagatctta
     7981 gccacttttt aaaagaaaag aggggactgg aagggctaat tcactcccaa cgaagacaag
     8041 atatccttga tctgtggatc taccacacac aaggctactt ccctgattag cagaactaca
     8101 caccagggcc aggggtcaga tatccactga cctttggatg gtgctacaag ctagtaccag
     8161 ttgagccaga taaggtagaa gaggccaata aaggagagaa caccagcttg ttacaccctg
     8221 tgagcctgca tgggatggat gacccggaga gagaagtgtt agagtggagg tttgacagcc
     8281 gcctagcatt tcatcacgtg gcccgagagc tgcatccgga gtacttcaag aactgctgat
     8341 atcgagcttg ctacaaggga ctttccgctg gggactttcc agggaggcgt ggcctgggcg
     8401 ggactgggga gtggcgagcc ctcagatcct gcatataagc agctgctttt tgcctgtact
     8461 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     8521 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     8581 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     8641 agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca
     8701 gagagtgaga ggccttgaca ttgctagcgt ttaccgtcga cctctagcta gagcttggcg
     8761 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     8821 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     8881 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     8941 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     9001 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     9061 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     9121 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     9181 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     9241 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     9301 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     9361 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     9421 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     9481 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     9541 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     9601 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     9661 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     9721 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     9781 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     9841 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     9901 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     9961 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
    10021 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
    10081 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
    10141 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
    10201 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
    10261 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
    10321 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
    10381 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
    10441 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
    10501 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
    10561 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
    10621 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
    10681 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
    10741 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
    10801 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
    10861 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
    10921 gacgtcgacg gatcgggaga tcaacttgtt tattgcagct tataatggtt acaaataaag
    10981 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
    11041 gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca
    11101 aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca
    11161 tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta
    11221 aatatgtact acaaacttag tagt
//
