LOCUS       sequence-301626-       11598 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express NSP2 gene along with HA and BASU.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11598)
  AUTHORS   Meyers JM, Ramanathan M, Shanderson RL, Beck A, Donohue L, Ferguson 
            I, Guo MG, Rao DS, Miao W, Reynolds D, Yang X, Zhao Y, Yang YY, 
            Blish C, Wang Y, Khavari PA
  TITLE     The proximal proteome of 17 SARS-CoV-2 proteins links to disrupted 
            antiviral signaling and host translation.
  JOURNAL   PLoS Pathog. 2021 Oct 1;17(10):e1009412. doi: 
            10.1371/journal.ppat.1009412.
  PUBMED    34597346
REFERENCE   2  (bases 1 to 11598)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Pathog."; date: "2021-10-1"; volume: "17(10):e1009412. doi"; pages: 
            " 10.1371/journal.ppat.1009412"
COMMENT     Sequence Label: SARS_COV2_nsp2_HA_BASU
FEATURES             Location/Qualifiers
     source          1..11598
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2337..3519
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2568..3510
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3467..3487
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3544..3553
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5485..5508
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             5512..5538
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    join(6348..6922,6923..6925,6926..6934)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6515..6532)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6742..6761
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6954..7553
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6954..6973)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7450..7470
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7567..8155
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7620..7640)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8038..8049)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8362..8995
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9123..9139)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9123..9139)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9136..9158)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9147..9163
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9171..9177,9178..9195,9196..9201))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9216..9237
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9354..9371)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9525..10113)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9605..9624)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10284..11075,11076..11144))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10907..10926
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11145..11249)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    11297..11431
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11334..11353)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11388..11407
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgagtaa ttcatacaaa aggactcgcc cctgccttgg
     2221 ggaatcccag ggaccgtcgt taaactccca ctaacgtaga acccagagat cgctgcgttc
     2281 ccgccccctc acccgcccgc tctcgtcatc actgaggtgg agaagagcat gcgtgaggct
     2341 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag
     2401 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg
     2461 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag
     2521 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt
     2581 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt
     2641 ccacgcccct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
     2701 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
     2761 cttgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
     2821 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
     2881 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
     2941 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
     3001 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
     3061 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
     3121 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
     3181 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
     3241 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
     3301 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     3361 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     3421 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     3481 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gatctatttc cggtgaattc
     3541 gccgccacca tggcctacac gcgatatgtt gacaacaact tttgtggacc tgacggctac
     3601 cctcttgaat gtattaagga tcttctggct cgcgctggaa aagccagttg tacactgtct
     3661 gagcagctcg actttataga tacaaagcgg ggggtctatt gttgccgaga gcatgaacat
     3721 gagatcgcat ggtatacgga gcgctctgaa aaaagctatg aattgcagac accctttgaa
     3781 attaagttgg cgaaaaagtt cgatacattc aatggtgagt gccccaactt tgtctttccc
     3841 cttaacagta tcattaaaac aatccagccc cgggtggaaa agaagaaatt ggatggattc
     3901 atgggcagga tcagaagtgt ttacccagtg gcatccccta atgaatgcaa tcagatgtgc
     3961 ctgtccacgc tgatgaaatg cgaccactgc ggtgaaacgt cttggcaaac gggggacttt
     4021 gtaaaagcaa cgtgcgagtt ttgcggaact gagaacttga caaaggaggg agcgactaca
     4081 tgtggatacc tgcctcagaa cgcggtagtg aaaatatact gtcctgcctg tcacaattct
     4141 gaagttgggc ctgaacatag cttggccgaa taccataacg aaagtggatt gaagacaatt
     4201 cttcgcaaag gaggccggac tatcgcgttc gggggctgcg tgtttagcta tgtaggatgt
     4261 cataataaat gcgcatattg ggttccacga gctagcgcaa acataggctg caaccacaca
     4321 ggcgtagttg gtgagggatc agaaggtctt aatgataact tgctcgaaat attgcaaaag
     4381 gaaaaagtca acataaatat tgtaggagat tttaaattga atgaagagat tgcgataatc
     4441 cttgcgtcat tttccgcaag tacgagtgcg ttcgttgaga ctgtaaaggg gctcgactat
     4501 aaagcgttta agcaaatagt agagagctgc ggaaacttta aagttacgaa gggaaaagcc
     4561 aaaaaaggtg catggaacat tggcgagcag aagagtatac tgtcccccct gtacgccttc
     4621 gcgtcagaag cagctcgagt agttagaagt atattctcac gaacgctcga gacggcgcag
     4681 aatagtgtgc gcgtgctcca gaaggctgct attacgatcc tcgacggcat atcccagtat
     4741 tctcttcgac tgattgatgc tatgatgttc acctccgact tggcgacgaa caatctcgta
     4801 gttatggctt atatcaccgg cggcgttgta caattgacgt ctcagtggct gacgaacatc
     4861 ttcggaaccg tgtacgagaa gctcaaacca gttcttgatt ggcttgaaga aaagtttaag
     4921 gaaggcgtcg agtttctccg ggatgggtgg gaaattgtta agtttatctc cacctgcgcg
     4981 tgtgaaattg tgggagggca aattgtcacc tgcgccaagg aaattaaaga gtcagttcag
     5041 acgtttttta aacttgtgaa caaattcctt gctctctgtg cggatagtat aatcatcggt
     5101 ggggcgaagt tgaaagcact caatcttggt gaaactttcg tgacccacag caaaggcctg
     5161 tataggaagt gtgttaaaag cagggaggag accggtcttc ttatgccgct gaaggcgccc
     5221 aaagagataa tttttcttga gggggaaact cttccgaccg aagtgctcac ggaagaagta
     5281 gttctgaaaa caggggacct tcaaccactg gagcaaccga cctctgaggc agttgaagcg
     5341 ccgctcgtcg gaactcctgt atgtattaac ggcctcatgc tgttggaaat taaggatacg
     5401 gagaagtact gtgcactggc ccccaacatg atggtcacga acaacacctt tacccttaag
     5461 ggcggcctcg aaggcggcgg gggatggagc catccacaat tcgagaaagg ctacccatac
     5521 gatgtgcctg actatgctgg caagttgtct gaatcagaga ttaggtttgg actgaaaacg
     5581 gaggtgatgg gccaacatct tatctatcac gatgttttgt ccagcacaca aaagacagcc
     5641 catgaattgg caaataacaa tgcccccgag ggtaccctcg tggttgcaga taaacagaca
     5701 gccggtaggg gcgggatgag ccgagtgtgg cattctcaag aaggaaacgg agtatggatg
     5761 agtttgatat tgcgcccgga cattccgctc caaaagacgc cccaacttac cttgctggca
     5821 gctgtagcgg tggttcaggg aatagaagag gccgcgggca tacagactga tataaagtgg
     5881 ccgaacgaca tactcattaa cgggaaaaaa acagtaggga tacttaccga aatgcaagct
     5941 gaggaggatc gagtgcgctc cgtgattatt ggtattggca ttaatgtaaa tcagcaaccc
     6001 aatgatttcc ccgatgagct caaggacatt gcgacgagtc tgtcccaggc agctggggaa
     6061 aagatcgaca gggcaggtgt aatccagcat atcttgcttt gtttcgaaaa gcgataccga
     6121 gactacatga cccacggttt cacgcccatc aagctcttgt gggagtcata cgccctcggt
     6181 attggcacga acatgagggc gagaactctc aacgggacat tttacggtaa ggcgttgggt
     6241 attgacgacg aaggggtact tttgttggaa actaatgaag gcattaagaa gatatacagc
     6301 gcggacatca gccttaggtg accgcagttt gaaaagtaag gatcccgccc ctctccctcc
     6361 ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat
     6421 atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct
     6481 gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg
     6541 ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta
     6601 gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag
     6661 ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg
     6721 atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat
     6781 gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca
     6841 tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc
     6901 ctttgaaaaa cacgatgata agcttgccac aacccacaag gagacgacct tccatgaccg
     6961 agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt cccccgggcc gtacgcaccc
     7021 tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgacccg gaccgccaca
     7081 tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca
     7141 aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg
     7201 aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc
     7261 tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt
     7321 ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg
     7381 tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct
     7441 ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg
     7501 aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tagacgcgtc
     7561 tggaacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     7621 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     7681 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     7741 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     7801 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     7861 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     7921 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg
     7981 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     8041 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     8101 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgga
     8161 attaattctg cagtcgagac ctagaaaaac atggagcaat cacaagtagc aatacagcag
     8221 ctaccaatgc tgattgtgcc tggctagaag cacaagagga ggaggaggtg ggttttccag
     8281 tcacacctca ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact
     8341 ttttaaaaga aaagagggga ctggaagggc taattcactc ccaacgaaga caagatatcc
     8401 ttgatctgtg gatctaccac acacaaggct acttccctga ttagcagaac tacacaccag
     8461 ggccaggggt cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc
     8521 cagataaggt agaagaggcc aataaaggag agaacaccag cttgttacac cctgtgagcc
     8581 tgcatgggat ggatgacccg gagagagaag tgttagagtg gaggtttgac agccgcctag
     8641 catttcatca cgtggcccga gagctgcatc cggagtactt caagaactgc tgatatcgag
     8701 cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg ggcgggactg
     8761 gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg tactgggtct
     8821 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     8881 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     8941 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt
     9001 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
     9061 gagaggcctt gacattgcta gcgtttaccg tcgacctcta gctagagctt ggcgtaatca
     9121 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     9181 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     9241 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     9301 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     9361 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     9421 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     9481 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     9541 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     9601 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     9661 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     9721 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     9781 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     9841 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     9901 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     9961 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
    10021 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
    10081 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
    10141 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
    10201 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
    10261 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
    10321 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
    10381 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
    10441 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
    10501 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
    10561 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
    10621 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
    10681 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
    10741 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
    10801 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
    10861 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
    10921 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
    10981 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
    11041 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
    11101 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
    11161 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
    11221 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
    11281 gacggatcgg gagatcaact tgtttattgc agcttataat ggttacaaat aaagcaatag
    11341 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
    11401 actcatcaat gtatcttatc atgtctggat caactggata actcaagcta accaaaatca
    11461 tcccaaactt cccaccccat accctattac cactgccaat tacctgtggt ttcatttact
    11521 ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt gttaaatatg
    11581 tactacaaac ttagtagt
//
