LOCUS       sequence-30163-e         649 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Sun Q, Fan W, Chen K, Ding X, Chen S, Zhong Q
  TITLE     Identification of Barkor as a mammalian autophagy-specific factor 
            for Beclin 1 and class III phosphatidylinositol 3-kinase.
  JOURNAL   Proc Natl Acad Sci U S A. 2008 Dec 9. 105(49):19211-6.
  PUBMED    19050071
REFERENCE   2  (bases 1 to 649)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2008 Dec 9. 105(49):19211-6."
COMMENT     Sequence Label: pET28a-UVRAG CC
FEATURES             Location/Qualifiers
     source          1..649
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             60..77
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             81..113
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             351..368
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(421..439)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      435..482
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(606..625)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ctttaagaag gagatatacc atgggcagca gccatcatca tcatnatnac agcagcggcc
       61 tggtgccgcg cggcagccat atggctagca tgactggtgg acagcaaatg ggtcgcggat
      121 ccagagccca gtgtgcaatt aaacagactc aggtaactgt tcagaaaatt ggaaaggaaa
      181 ttgaagaaaa actaagactc acatctacaa gcaatgaact gaaaaaaaaa agtgaatgcc
      241 tgcagttaaa aattttggtg cttcagaatg aactggaacg gcagaagaaa gctttgggac
      301 gggaggtggc attactgcat aagcaacaaa tttgagcggc cgcactcgag caccaccacc
      361 accaccactg agatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca
      421 ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt
      481 tgctgaaagg aggaactata tccggattgg cgaatgggac gcgccctgta gcggcgcatt
      541 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
      601 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggc
//
