LOCUS       sequence-30164-e         614 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Sun Q, Fan W, Chen K, Ding X, Chen S, Zhong Q
  TITLE     Identification of Barkor as a mammalian autophagy-specific factor 
            for Beclin 1 and class III phosphatidylinositol 3-kinase.
  JOURNAL   Proc Natl Acad Sci U S A. 2008 Dec 9. 105(49):19211-6.
  PUBMED    19050071
REFERENCE   2  (bases 1 to 614)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2008 Dec 9. 105(49):19211-6."
COMMENT     Sequence Label: pET28a-Beclin1 (1-242)
FEATURES             Location/Qualifiers
     source          1..614
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(27..44)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cgggctttgt tagcagccgg atctcagtgg tggtggtggt ggtgctcgag tgcggccgct
       61 cactccagct gctgtcgttt aaattcactg tattctctct gatactgagc ttcctcctga
      121 tccagtctct cagcctcagc ctggaccttc tcgagatttt ctgccactat cttgcggttc
      181 ttttccacgt cttccagctc ctggatcagc ctctcctcct ctagtgccag ctcctttagc
      241 tccatctgta actgttcact gtcatcctca ttcatttgct ctaagatctc caaacagcgt
      301 ttgtagttct gacactcatt ttcagtgacg ttgagctgag tgtccagctg gtctaaaaga
      361 gtatctgtgc attcctcaca gagtgggtga tccacatctg tctggcccga catgatgtca
      421 aaaaggtccc cagtgacctt cagtcttcgg ctgaggttct ccatggtgcc gccatcagat
      481 gcctccccaa tcagagtgaa gctgttggca ctttctgtgg acatcatcct ggctgggggg
      541 atgaatctgc gagagacacc atcctggcga ggagtttcaa taaatggctc ctctcctgag
      601 ttagtctctt cctc
//
