LOCUS       sequence-30165-e         663 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 663)
  AUTHORS   Sun Q, Fan W, Chen K, Ding X, Chen S, Zhong Q
  TITLE     Identification of Barkor as a mammalian autophagy-specific factor 
            for Beclin 1 and class III phosphatidylinositol 3-kinase.
  JOURNAL   Proc Natl Acad Sci U S A. 2008 Dec 9. 105(49):19211-6.
  PUBMED    19050071
REFERENCE   2  (bases 1 to 663)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2008 Dec 9. 105(49):19211-6."
COMMENT     Sequence Label: pET28a-Beclin1 (1-242)
FEATURES             Location/Qualifiers
     source          1..663
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             70..87
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             91..123
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatatacc atgggcagca gccatcatca tcntnntcac
       61 agcagcggcc tggtgccgcg cggcagccat atggctagca tgactggtgg acagcaaatg
      121 ggtcgcggat ccatggaagg gtctaagacg tccaacaaca gcaccatgca ggtgagcttc
      181 gtgtgccagc gctgcagcca gcccctgaaa ctggacacga gtttcaagat cctggaccgt
      241 gtcaccatcc aggaactcac agctccatta cttaccacag cccaggcgaa accaggagag
      301 acccaggagg aagagactaa ctcaggagag gagccattta ttgaaactcc tcgccaggat
      361 ggtgtctctc gcagattcat ccccccagcc aggatgatgt ccacagaaag tgccaacagc
      421 ttcactctga ttggggaggc atctgatggc ggcaccatgg agaacctcag ccgaagactg
      481 aaggtcactg gggacctttt tgacatcatg tcgggccaga cagatgtgga tcacccactc
      541 tgtgaggaat gcacagatac tcttttagac cagctggaca ctcagctcaa cgtcactgaa
      601 aatgagtgtc agaactacaa acgctgtttg gagatcttag agcaaatgaa tgaggatgac
      661 agt
//
