LOCUS       sequence-30167-e         763 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Sun Q, Fan W, Chen K, Ding X, Chen S, Zhong Q
  TITLE     Identification of Barkor as a mammalian autophagy-specific factor 
            for Beclin 1 and class III phosphatidylinositol 3-kinase.
  JOURNAL   Proc Natl Acad Sci U S A. 2008 Dec 9. 105(49):19211-6.
  PUBMED    19050071
REFERENCE   2  (bases 1 to 763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2008 Dec 9. 105(49):19211-6."
COMMENT     Sequence Label: pET28a-Beclin1 CC
FEATURES             Location/Qualifiers
     source          1..763
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             5..27
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             46..63
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             73..90
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             94..126
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             430..447
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(500..518)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      514..561
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(685..704)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 taattttgtt taactttaag aaggagatat accatgggca gcagccatca tcatcatcat
       61 cacagcagcg gcctggtgcc gcgcggcagc catatggcta gcatgactgg tggacagcaa
      121 atgggtcgcg gatcccagct caacgtcact gaaaatgagt gtcagaacta caaacgctgt
      181 ttggagatct tagagcaaat gaatgaggat gacagtgaac agttacagat ggagctaaag
      241 gagctggcac tagaggagga gaggctgatc caggagctgg aagacgtgga aaagaaccgc
      301 aagatagtgg cagaaaatct cgagaaggtc caggctgagg ctgagagact ggatcaggag
      361 gaagctcagt atcagagaga atacagtgaa tttaaacgac agcagctgga gtgagcggcc
      421 gcactcgagc accaccacca ccaccactga gatccggctg ctaacaaagc ccgaaaggaa
      481 gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa
      541 cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggattggc gaatgggacg
      601 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
      661 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
      721 tcgccggctt tccccgtcaa gctctaaatc gggggctccc ttt
//
