LOCUS       sequence-301670-       10325 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for SARS-CoV-2 S (surface 
            glycoprotein).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10325)
  TITLE     SARS-CoV-2 protein Mammalian expression constructs, tagged, for 
            protein expression and/or purification
REFERENCE   2  (bases 1 to 10325)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4252844
FEATURES             Location/Qualifiers
     source          1..10325
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(2824..3026)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2853..2873)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        3028..3331
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     polyA_signal    3412..3460
                     /note="synthetic polyadenylation signal"
     terminator      3478..3524
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     misc_feature    3529..3600
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(3752..3888)
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     CDS             complement(join(3901..4692,4693..4761))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4524..4543
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     polyA_signal    5027..5075
                     /note="synthetic polyadenylation signal"
     CDS             complement(5102..5701)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5185..5205)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     5682..5701
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(5705..6914)
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     complement(5849..5868)
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     rep_origin      7414..8002
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7903..7922
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    8228..8299
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(8344..8480)
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     terminator      complement(8510..8556)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    8671..8792
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8708..8727)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8762..8781
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(8869..8886)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(9235..9255)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 agagaacatt ctgtgtaact ccaataccat taaacctata agccatttgc atagcaaatg
       61 gtatttgtaa tgcagcacct gcaccaaagg tccaaccaga agtgattgta cccgctaaca
      121 gtgcagaagt gtattgagca atcatttcat ctgtgagcaa aggtggcaaa acagtaaggc
      181 cgttaaactt ttgtgcacaa atgaggtctc tagcagcaat atcaccaagg caatcaccat
      241 attgtttgat gaagccagca tctgcaagtg tcactttgtt gaaaagtaga tcttcaataa
      301 atgacctctt gcttggtttt gatggatctg gtaatatttg tgaaaaatta aaaccaccaa
      361 aatctttaat tggtggtgtt ttgtaaattt gtttgacttg tgcaaaaact tcttgggtgt
      421 ttttgtcttg ttcaacagct attccagtta aagcacggtt taattgtgta caaaaactgc
      481 catattgcaa caaaagattg ctgcattcag ttgaatcacc acaaatgtac attgtacaat
      541 ctactgatgt cttggtcata gacactggta gaatttctgt ggtaacacta atagtaaaat
      601 ttgtgggtat ggcaatagag ttattagagt aagcaactga attttctgca ccaagtgaca
      661 tagtgtaggc aatgatggat tgactagcta cactacgtgc ccgccgagga gaattagtct
      721 gagtctgata actagcgcat atacctgcac caatgggtat gtcacactca tatgagttgt
      781 tgacatgttc agcccctatt aaacagcctg cacgtgtttg aaaaacatta gaacctgtag
      841 aataaacacg ccaagtagga gtaagttgat ctgcatgaat agcaacaggg acttctgtgc
      901 agttaacatc ctgataaaga acagcaacct ggttagaagt atttgttcct ggtgttataa
      961 cactgacacc accaaaagaa catggtgtaa tgtcaagaat ctcaagtgtc tgtggatcac
     1021 ggacagcatc agtagtgtca gcaatgtctc tgccaaattg ttggaaaggc agaaactttt
     1081 tgttagactc agtaagaaca cctgtgcctg ttaaaccatt gaagttgaaa ttgacacatt
     1141 tgtttttaac caaattagta gactttttag gtccacaaac agttgctggt gcatgtagaa
     1201 gttcaaaaga aagtactact actctgtatg gttggtaacc aacaccatta gtgggttgga
     1261 aaccatatga ttgtaaagga aagtaacaat taaaaccttc aacaccatta caaggtgtgc
     1321 taccggcctg atagatttca gttgaaatat ctctctcaaa aggtttgaga ttagacttcc
     1381 taaacaatct atacaggtaa ttataattac caccaacctt agaatcaaga ttgttagaat
     1441 tccaagctat aacgcagcct gtaaaatcat ctggtaattt ataattataa tcagcaatct
     1501 ttccagtttg ccctggagcg atttgtctga cttcatcacc tctaattaca aatgaatctg
     1561 catagacatt agtaaagcag agatcattta atttagtagg agacactcca taacacttaa
     1621 aagtggaaaa tgatgcggaa ttatatagga cagaataatc agcaacacag ttgctgattc
     1681 tcttcctgtt ccaagcataa acagatgcaa atctggtggc gttaaaaact tcaccaaaag
     1741 ggcacaagtt tgtaatatta ggaaatctaa caatagattc tgttggttgg actctaaagt
     1801 tagaagtttg atagattcct ttttctacag tgaaggattt caacgtacac tttgtttctg
     1861 agagagggtc aagtgcacag tctacagcat ctgtaatggt tccattttca ttatatttta
     1921 atagaaaagt cctaggttga agataaccca cataataagc tgcagcacca gctgtccaac
     1981 ctgaagaaga atcaccagga gtcaaataac ttctatgtaa agcaagtaaa gtttgaaacc
     2041 tagtgatgtt aatacctatt ggcaaatcta ccaatggttc taaagccgaa aaaccctgag
     2101 ggagatcacg cactaaatta ataggcgtgt gcttagaata tattttaaaa taaccatcaa
     2161 tattcttaaa cacaaattcc ctaagatttt tgaaattacc ctgttttcct tcaaggtcca
     2221 taagaaaagg ctgagagaca tattcaaaag tgcaattatt cgcactagaa taaactctga
     2281 actcactttc catccaactt ttgttgtttt tgtggtaata aacacccaaa aatggatcat
     2341 tacaaaattg aaattcacag actttaataa caacattagt agcgttatta acaataagta
     2401 gggactgggt cttcgaatct aaagtagtac caaaaatcca gcctcttatt atgttagact
     2461 tctcagtgga agcaaaataa acaccatcat taaatggtag gacagggtta tcaaacctct
     2521 tagtaccatt ggtcccagag acatgtatag catggaacca agtaacattg gaaaagaaag
     2581 gtaagaacaa gtcctgagtt gaatgtaaaa ctgaggatct gaaaactttg tcagggtaat
     2641 aaacaccacg tgtgaaagaa ttagtgtatg cagggggtaa ttgagttctg gttgtaagat
     2701 taacacactg actagagact agtggcaata aaacaagaaa aacaaacatg gtggcggccg
     2761 ctggcgggtg tgtcgagtgg atggtaggat cgacaaagat ctgatctgac ggttcactaa
     2821 accagctctg cttatataga cctcccaccg tacacgccta ccgcccattt gcgtcaatgg
     2881 ggcggagttg ttacgacatt ttggaaagtc ccgttgattt tggtgccaaa acaaactccc
     2941 attgacgtca atggggtgga gacttggaaa tccccgtgag tcaaaccgct atccacgccc
     3001 attgatgtac tgccaaaacc gcatcagcat ggtaatagcg atgactaata cgtagatgta
     3061 ctgccaagta ggaaagtccc ataaggtcat gtactgggca taatgccagg cgggccattt
     3121 accgtcattg acgtcaatag ggggcgtact tggcatatga tacacttgat gtactgccaa
     3181 gtgggcagtt taccgtaaat actccaccca ttgacgtcaa tggaaagtcc ctattggcgt
     3241 tactatggga acatacgtca ttattgacgt caatgggcgg gggtcgttgg gcggtcagcc
     3301 aggcgggcca tttaccgtaa gttatgtaac gcggaactcc atatatgggc tatgaactaa
     3361 tgaccccgta attgattact agatctggct acactcgatc agcagttaga taataaaatc
     3421 gctatccatc gaagatggat gtgtgttggt tttttgtgtg tgtaacgcaa cgattgatag
     3481 cataacccct tggggcctct aaacgggtct tgaggggttt tttgaattga gaggtagctg
     3541 aagctgctgt tatgaccact agagggaaga agatacctgt ggagctaatg gtccaagatg
     3601 tcgggagccg cgatcgctgc caaaaaggta agctgacgat agctgacgag attcgatgcc
     3661 cgacgagcaa atgagcgcca cggctgttcg cgtaagctcg aaacgacgga gattcgatag
     3721 agccagagtc gagtttaaac gattcgggat cttaaggata aatttctggt aaggaggaca
     3781 cgtatggaag tgggcaagtt ggggaagccg tatccgttgc tgaatctggc atatgtggga
     3841 gtataagacg cgcagcgtcg catcaggcat ttttttctgc gccaatgcac ctagaatcgg
     3901 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     3961 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     4021 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     4081 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     4141 gtctatcaac tgttgccggg aagctagagt aagtagttcg ccagttaata gtttacgcaa
     4201 ggttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     4261 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     4321 ggttagctcc ttcggtcctc caatcgttgt cagaagtaag ttggccgcag tgttatcact
     4381 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     4441 tgtgactggt gaatactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     4501 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     4561 catcattgga aagcgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     4621 cagttcgatg taacccactc gcgcacccaa ctgatcttca gcatctttta ctttcaccag
     4681 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     4741 acggaaatgt tgaatactca tgcgaaagcc tcctgctgat cctgtctctt gatagatgtt
     4801 tgcaaaagct aggactcgat tatacgttga ccgatcagac tatcaatatc taggtttaaa
     4861 cagatcggta cgctgccgtg accaggcttg agtacaatat ctgacgattg gacttcctgg
     4921 gtcccaagct ggattccgac tggctgactg ccaagcaacg atatggatcg tcgagactcg
     4981 cactgagagg atcgttctcg gtacaccagg cgcgcctagc gttacacaca caaaaaacca
     5041 acacacatcc atcttcgatg gatagcgatt ttattatcta agactaggct tacatactag
     5101 cctaggcacc gggcttgcgg gtcatgcacc aggtgcgcgg tccttcgggc acctcgacgt
     5161 cggcggtgac ggtgaagccg agccgctcgt agaaggggag gttgcggggc gcggaggtct
     5221 ccaggaaggc gggcaccccg gcgcgctcgg cggcctccac tccggggagc acgacggcgc
     5281 tgcccagacc cttgccctgg tggtcgggcg agacgccgac ggtggccagg aaccacgcgg
     5341 gctccttggg ccggtgcggc gccaggaggc cctccatctg ttgctgcgcg gccagccggg
     5401 aaccgctcaa ctcggccatg cgcgggccga tctcggcgaa caccgccccc gcttcgacgc
     5461 tctccggcgt ggtccagacc gccaccgcgg cgccgtcgtc cgcgacccac accttgccga
     5521 tgtcgagccc gacgcgcgtg aggaagagtt cttgcagctc ggtgacccgc tcgatgtggc
     5581 ggtccggatc gacggtgtgg cgcgtggcgg ggtagtcggc gaacgcggcg gcgagggtgc
     5641 gtacggccct ggggacgtcg tcgcgggtgg cgaggcgcac cgtgggcttg tactcggtca
     5701 tgttgtctaa caaaaaagcc aaaaacggcc agaatttagc ggacaattta caagtctaac
     5761 actgaaaatt acatattgac ccaaatgatt acatttcaaa aggtgcctaa aaaacttcac
     5821 aaaacacact cgccaacccc gagcgcatag ttcaaaaccg gagcttcagc tacttaagaa
     5881 gataggtaca taaaaccgac caaagaaact gacgcctcac ttatccctcc cctcaccaga
     5941 ggtccggcgc ctgtcgattc aggagagcct accctaggcc cgaaccctgc gtcctgcgac
     6001 ggagaaaagc ctaccgcaca cctaccggct ggtggcccca ccctgcatta taagccaaca
     6061 gaacgggtga cgtcacgaca cgacgagggc gcgcgctccc aaaggtacgg gtgcactgcc
     6121 caacggcacc gccataactg ccgcccccgc aacagacgac aaaccgagtt ctccagtcag
     6181 tgacaaactt cacgtcaggg tccccagatg gtgcccagcc catctcaccc gaataagagc
     6241 tttcccgcat tagcgaaggg ctcaagacct tgggttcttg ccgcccacca tgccccccac
     6301 cttgtttcaa cgacctcaca gcccgcctca caagcgtctt ccattcaaga ctcgggaaca
     6361 gccgccattt tgctgcgctc cccccaaccc ccagttcagg gcaaccttgc tcgcggaccc
     6421 agactacagc ccttggcggt ctctccacac gcttccgtcc caccgagcgg cccggcggcc
     6481 acgaaagccc cggccagccc agcagcccgc tactcaccaa gtgacgatca cagcgatcca
     6541 caaacaagaa ccgcgaccca aatcccggct gcgacggaac tagctgtgcc acacccggcg
     6601 cgtccttata taatcatcgg cgttcaccgc cccacggaga tccctccgca gaatcgccga
     6661 gaagggacta cttttcctcg cctgttccgc tctctggaaa gaaaaccagt gccctagagt
     6721 cacccaagtc ccgtcctaaa atgtccttct gctgatactg gggttctaag gccgagtctt
     6781 atgagcagcg ggccgctgtc ctgagcgtcc gggcggaagg atcaggacgc tcctgcgccc
     6841 ttcgtctgac gtggcagcgc tcgccgtgac gaggggggcg cccgcgggag gcgccaaaac
     6901 ccggcgcgga ggccaggcgc gccccgattt cgatattcct cggatctcgg atagatcggg
     6961 attgaggatg taggttgaac ttctctcggg tgatcccgat ctcggatatt cgggattgga
     7021 gtaatacctg gcgataggcg ctgccaagca atagccaagg cgcaaggccg gccaagagac
     7081 ttgcgatagt tcgttcgaga ggtagcctgc aagatggatc ggacgaaagg tttgaagatt
     7141 ttccgacgag ggccttggag agttcggaaa tttaaatgca actatggatg aacgaaatag
     7201 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     7261 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     7321 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     7381 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     7441 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     7501 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     7561 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     7621 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     7681 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     7741 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     7801 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     7861 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     7921 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     7981 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     8041 ttgctggcct tttgctcaca tgatttaaat gaaactcgta atcggagaac gggtgcaata
     8101 gcaaggacca gatcgaactt gactgacgca attctgagcg tagtcgaaac ccagacaagt
     8161 cgatctcgcc aagtagctga cttgactggt taattaagtt tcgatagccc aaggtaacca
     8221 atgagattag ggagggaaga aagtttgatg aactacttct gacccctagt ggtgtccaga
     8281 aaagaccatt aaaggaatgt cttgatcgaa atcgagagat cgcctgcagg atagctgacg
     8341 accttaagga taaatttctg gtaaggagga cacgtatgga agtgggcaag ttggggaagc
     8401 cgtatccgtt gctgaatctg gcatatgtgg gagtataaga cgcgcagcgt cgcatcaggc
     8461 atttttttct gcgccaatgc cggtagatag caatccggcc cgaggggcac aaaaaacccc
     8521 tcaagacccg tttagaggcc ccaaggggtt atgctacgat accgatagag atgggccaat
     8581 accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg
     8641 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac
     8701 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     8761 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtgagctgaa ggtacgctgt
     8821 atctcagtca gtcaagctag cttagccgcg tggcacgagc ccgctgctat ggtgatggtg
     8881 atggtgcatg tgccccgagc ctgaacctgc caggttagcg tccaagaact cttttagctg
     8941 gcccttagac agggctccca cttttgtcgc ggcaacttcc ccattcttaa agagcaggag
     9001 ggtaggtatt cccctgatgc cgtatttagg tgccgtccct gggttctgat ctatattaag
     9061 cttagcgaca gttaattttc cttgatactc gtcggcgatc tcgtccagaa tgggtgcgat
     9121 catcttgcac gggccacacc attcagccca gaaatctacc agtatcgcac catcggcctt
     9181 caacacgtct gtatcaaagg agtcatcggt tagatgaatg attttgtcac tcatcgactg
     9241 aaagtaaagg ttttctgtgt aatgtaattt gactcctttg agcactggct cagagtcgtc
     9301 ttcatcaaat ttgcagcagg atccacaaga acaacagccc ttgagacaac tacagcaact
     9361 ggtcatacag caaagcataa ttgtcaccat tactatggca atcaagccag ctataaaacc
     9421 tagccaaatg taccatggcc attttatata ctgctcatac tttccaagtt cttggagatc
     9481 gatgagagat tcatttaaat tcttggcaac ctcattgagg cggtcaattt ctttttgaat
     9541 gtttacaact gaagcattaa tgccagagat gtcacctaaa tcaacatctg gtgatgtatg
     9601 attcttaaaa tatttatcta actcctcctt gaatgagtct aattcaggtt gcaaaggatc
     9661 ataaactgtg ttgttgacaa ttcctattac aacatcacag ttaccagaca caaatgtgtt
     9721 gtctgtagta atgatttgtg gttcataaaa attcctttgt gttacaaacc agtgtgtgcc
     9781 atttgaaaca aagacacctt cacgaggaaa gtgtgctttt ccatcatgac aaatggcagg
     9841 agcagttgtg aagttctttt cttgtgcagg gacataagtc acatgcaaga agactacacc
     9901 atgaggtgct gactgaggga aggacataag atgatagccc tttccacaaa aatcaactct
     9961 ttttgattgt ccaagtacac actctgacat tttagtagca gcaagattag cagaagctct
    10021 gatttctgca gctctaatta attgttgagt cacatatgtc tgcaaacttt gaagtctgcc
    10081 tgtgatcaac ctatcaattt gcacttcagc ctcaactttg tcaagacgtg aaaggatatc
    10141 atttaaaaca cttgaaattg caccaaaatt ggagctaagt tgtttaacaa gcgtgtttaa
    10201 agcttgtgca ttttggttga ccacatcttg aagttttcca agtgcacttg ctgtggaaga
    10261 aagtgagtct tgaattttgc caatagcact attaaattgg ttggcaatca atttttggtt
    10321 ctcat
//
