LOCUS       sequence-30172-e         576 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 576)
  AUTHORS   Hsiao EC, Nguyen TD, Ng JK, Scott MJ, Chang WC, Zahed H, Conklin BR
  TITLE     Constitutive Gs activation using a single-construct 
            tetracycline-inducible expression system in embryonic stem cells and
            mice.
  JOURNAL   Stem Cell Res Ther. 2011 Mar 4. 2(2):11.
  PUBMED    21375737
REFERENCE   2  (bases 1 to 576)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Res Ther. 2011 Mar 4. 2(2):11."
COMMENT     Sequence Label: pEnt L1L3 tTA-2
FEATURES             Location/Qualifiers
     source          1..576
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    68..92
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(125..158)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gattttattt tgactgatag tgacctgttc gttgcaacaa attgataagc aatgcttttt
       61 tataatgcca actttgtaca aaaaagcagg ctggcgccgg aaccaattca gtcgacgttt
      121 aaacataact tcgtatagca tacattatac gaagttatct cgagcctcag gaaacagggt
      181 accagtgaaa gtctgcaatg aattcgagtt ggcgcgcctg tcattctaaa tctctctttc
      241 agcctaaagc tttttccccg tatcccccca ggtgtctgca ggctcaaaga gcagcgagaa
      301 gcgttcagag gaaagcgatc ccgtgccacc ttccccgtgc ccgggctgtc cccgcacgct
      361 gccggctcgg ggatgcgggg ggagcgccgg accggagcgg agccccgggc ggctcgctgc
      421 tgccccctag cgggggaggg acgtaattac atccctgggg gctttgggag ggggctgtcc
      481 ccgtgagctc ccaggcgcgc ctgtcattct aaatctctct ttcagcctaa agctttttcc
      541 ccgtatcccc ccaggtgtct gcaggctcaa agagca
//
