LOCUS       sequence-30175-e         646 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 646)
  AUTHORS   Hsiao EC, Nguyen TD, Ng JK, Scott MJ, Chang WC, Zahed H, Conklin BR
  TITLE     Constitutive Gs activation using a single-construct 
            tetracycline-inducible expression system in embryonic stem cells and
            mice.
  JOURNAL   Stem Cell Res Ther. 2011 Mar 4. 2(2):11.
  PUBMED    21375737
REFERENCE   2  (bases 1 to 646)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Res Ther. 2011 Mar 4. 2(2):11."
COMMENT     Sequence Label: pEnt R3L2 TetO(fl)-2
FEATURES             Location/Qualifiers
     source          1..646
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(98..118)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     polyA_signal    145..193
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 gactggatat gttgtgtttt acagtattat gtagtctgtt ttttatgcaa aatctaattt
       61 aatatattga tatttatatc attttacgtt tctcgttcaa ctttattata catagttgct
      121 taagatctct gaatgcggcc gcttcacaca aaaaaccaac acacagatgt aatgaaaata
      181 aagatatttt attgcggcca tcgtgatggc aggttgggcg tcgcttggtc ggtcatttcg
      241 aaccccagag tcccgcctgc aggccggcct agtgcagggc gtcgacgaat tctttgccaa
      301 aatgatgaga cagcacaaca accagcacgt tgcccaggag ctgtaggaaa gagaagaagg
      361 catgaacatg gttagcagag gggcccggtt tggactcaga gtattttatc ctcatctcaa
      421 acagtgtata tcattgtaac cataaagaga aaggcaggat gatgaccagg gtgtagttgt
      481 ttctaccaat aagaatattt ccacgccagc cagaatttat atgcagaaat attctacctt
      541 atcatttaat tataacaatt gttctctaaa actgtgctga agtacaatat aatataccct
      601 gattgccttg aaaaaaaagt gattagagaa agtacttaca atctga
//
